1EZ3

CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1ez3a_	All alpha proteins	STAT-like	t-snare proteins	t-snare proteins	Syntaxin 1A N-terminal domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
C	d1ez3c_	All alpha proteins	STAT-like	t-snare proteins	t-snare proteins	Syntaxin 1A N-terminal domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
B	d1ez3b_	All alpha proteins	STAT-like	t-snare proteins	t-snare proteins	Syntaxin 1A N-terminal domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	t-snare proteins	8025203	4000986	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	t-snare proteins	8037582	3000366	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	t-snare proteins	8037582	3000366	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	t-snare proteins	8037582	3000366	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Syntaxin	e1ez3A1	A: alpha bundles	X: STAT-like	H: t-snare proteins (From Topology)	T: t-snare proteins	F: Syntaxin	ECOD (1.6)
C	Syntaxin	e1ez3C1	A: alpha bundles	X: STAT-like	H: t-snare proteins (From Topology)	T: t-snare proteins	F: Syntaxin	ECOD (1.6)
B	Syntaxin	e1ez3B1	A: alpha bundles	X: STAT-like	H: t-snare proteins (From Topology)	T: t-snare proteins	F: Syntaxin	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.58.70	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
C	1.20.58.70	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
B	1.20.58.70	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF00804	Syntaxin (Syntaxin)	Syntaxin	Syntaxins are the prototype family of SNARE proteins. They usually consist of three main regions - a C-terminal transmembrane region, a central SNARE domain which is characteristic of and conserved in all syntaxins (Pfam:PF05739), and an N-terminal d ...	Syntaxins are the prototype family of SNARE proteins. They usually consist of three main regions - a C-terminal transmembrane region, a central SNARE domain which is characteristic of and conserved in all syntaxins (Pfam:PF05739), and an N-terminal domain that is featured in this entry. This domain varies between syntaxin isoforms; in syntaxin 1A (Swiss:O35526) it is found as three alpha-helices with a left-handed twist. It may fold back on the SNARE domain to allow the molecule to adopt a 'closed' configuration that prevents formation of the core fusion complex - it thus has an auto-inhibitory role. The function of syntaxins is determined by their localisation. They are involved in neuronal exocytosis, ER-Golgi transport and Golgi-endosome transport, for example. They also interact with other proteins as well as those involved in SNARE complexes. These include vesicle coat proteins, Rab GTPases, and tethering factors [6].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	SYNTAXIN-1A	molecular function inhibitor activity chloride channel inhibitor activity channel regulator activity transporter inhibitor activity ion channel regulator activity ion channel inhibitor activity molecular function regulator activity transporter regulator activity channel inhibitor activity chloride channel regulator activity SNAP receptor activity protein-macromolecule adaptor activity molecular adaptor activity protein-containing complex binding molecular function inhibitor activity chloride channel inhibitor activity channel regulator activity transporter inhibitor activity ion channel regulator activity ion channel inhibitor activity molecular function regulator activity transporter regulator activity channel inhibitor activity chloride channel regulator activity SNAP receptor activity protein-macromolecule adaptor activity molecular adaptor activity protein-containing complex binding binding calcium channel inhibitor activity calcium channel regulator activity identical protein binding protein binding myosin heavy chain binding cytoskeletal protein binding myosin head/neck binding myosin binding ATP-dependent protein binding kinase binding enzyme binding transmembrane transporter binding SNARE binding calcium-dependent protein binding	localization transport neurotransmitter transport establishment of localization cellular response to stimulus multicellular organismal process regulation of postsynaptic membrane potential system process regulation of cellular process response to stimulus signaling modulation of chemical synaptic transmission regulation of system process regulation of signal transduction localization transport neurotransmitter transport establishment of localization cellular response to stimulus multicellular organismal process regulation of postsynaptic membrane potential system process regulation of cellular process response to stimulus signaling modulation of chemical synaptic transmission regulation of system process regulation of signal transduction chemical synaptic transmission, postsynaptic cell communication synaptic signaling modulation of excitatory postsynaptic potential regulation of multicellular organismal process signal transduction regulation of biological quality trans-synaptic signaling regulation of biological process regulation of signaling cell surface receptor signaling pathway nervous system process biological regulation cell-cell signaling chemical synaptic transmission regulation of nervous system process regulation of trans-synaptic signaling regulation of response to stimulus anterograde trans-synaptic signaling regulation of cell communication cellular process excitatory postsynaptic potential regulation of membrane potential positive regulation of cell communication positive regulation of signal transduction positive regulation of excitatory postsynaptic potential positive regulation of biological process positive regulation of response to stimulus positive regulation of cellular process positive regulation of signaling regulated exocytosis secretion export from cell vesicle-mediated transport secretion by cell exocytosis calcium-ion regulated exocytosis membrane organization protein-containing complex organization membrane fusion organelle membrane fusion cellular component organization cellular component biogenesis vesicle organization organelle fusion organelle organization cellular component assembly protein-containing complex assembly SNARE complex assembly cellular component organization or biogenesis vesicle fusion peptidyl-lysine modification primary metabolic process protein sumoylation macromolecule modification peptidyl-amino acid modification metabolic process protein metabolic process macromolecule metabolic process protein modification process post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal monoamine transport regulation of localization nitrogen compound transport regulation of catecholamine secretion organic hydroxy compound transport positive regulation of secretion by cell positive regulation of amine transport regulation of secretion by cell catecholamine transport positive regulation of secretion regulation of transport positive regulation of catecholamine secretion regulation of secretion catecholamine secretion amine transport positive regulation of transport regulation of amine transport regulation of calcium ion-dependent exocytosis positive regulation of exocytosis regulation of regulated secretory pathway positive regulation of regulated secretory pathway positive regulation of calcium ion-dependent exocytosis regulation of vesicle-mediated transport regulation of exocytosis protein localization cellular localization cellular macromolecule localization macromolecule localization localization within membrane protein localization to membrane regulation of hormone levels hormone secretion signal release hormone transport regulation of cellular component organization synaptic vesicle priming regulation of synaptic vesicle priming synaptic vesicle cycle synaptic vesicle exocytosis vesicle-mediated transport in synapse regulation of protein-containing complex assembly establishment of localization in cell neurotransmitter secretion regulation of cellular component biogenesis signal release from synapse exocytic process response to abiotic stimulus response to gravity establishment of protein localization protein localization to extracellular region peptide secretion amide transport establishment of protein localization to extracellular region protein secretion peptide transport protein transport peptide hormone secretion insulin secretion organelle localization by membrane tethering organelle localization vesicle docking membrane docking vesicle docking involved in exocytosis synaptic vesicle docking synaptic vesicle endocytosis import into cell presynaptic endocytosis synaptic vesicle recycling endocytosis intracellular transport intracellular protein transport positive regulation of neurotransmitter secretion positive regulation of neurotransmitter transport regulation of neurotransmitter transport positive regulation of synaptic transmission regulation of neurotransmitter secretion endomembrane system organization vesicle fusion to plasma membrane synaptic vesicle fusion to presynaptic active zone membrane synaptic vesicle membrane organization norepinephrine transport positive regulation of norepinephrine secretion organic cation transport norepinephrine secretion regulation of norepinephrine secretion	postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane cellular anatomical entity postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse neuron to neuron synapse organelle cell junction postsynaptic specialization membrane postsynaptic specialization synapse membrane plasma membrane cellular anatomical entity postsynaptic density membrane synaptic membrane cell periphery postsynaptic density postsynapse neuron to neuron synapse organelle cell junction postsynaptic membrane asymmetric synapse plasma membrane region membrane-bounded organelle intracellular anatomical structure nuclear membrane nucleus intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope nuclear envelope organelle membrane cytoskeleton intracellular non-membrane-bounded organelle actin cytoskeleton actomyosin non-membrane-bounded organelle presynapse presynaptic membrane intracellular vesicle cytoplasmic vesicle secretory granule secretory vesicle cytoplasm vesicle glutamatergic synapse cell projection neuron projection plasma membrane bounded cell projection SNARE complex protein-containing complex synaptobrevin 2-SNAP-25-syntaxin-1a complex membrane protein complex synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex transport vesicle membrane bounding membrane of organelle vesicle membrane synaptic vesicle synaptic vesicle membrane transport vesicle exocytic vesicle membrane cytoplasmic vesicle membrane exocytic vesicle axon synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex acrosomal vesicle presynaptic active zone membrane presynaptic active zone Schaffer collateral - CA1 synapse

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR000727	Target SNARE coiled-coil homology domain	Domain
A, B, C	IPR006012	Syntaxin/epimorphin, conserved site	Conserved Site
A, B, C	IPR010989	SNARE	Homologous Superfamily
A, B, C	IPR006011	Syntaxin, N-terminal domain	Domain
A, B, C	IPR045242	Syntaxin	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.