Annotations: 1F80

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1f80a_	Alpha and beta proteins (a+b)	4'-phosphopantetheinyl transferase	4'-phosphopantetheinyl transferase	Holo-(acyl carrier protein) synthase ACPS	Holo-(acyl carrier protein) synthase ACPS	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d1f80b_	Alpha and beta proteins (a+b)	4'-phosphopantetheinyl transferase	4'-phosphopantetheinyl transferase	Holo-(acyl carrier protein) synthase ACPS	Holo-(acyl carrier protein) synthase ACPS	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
C	d1f80c_	Alpha and beta proteins (a+b)	4'-phosphopantetheinyl transferase	4'-phosphopantetheinyl transferase	Holo-(acyl carrier protein) synthase ACPS	Holo-(acyl carrier protein) synthase ACPS	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
E	d1f80e_	All alpha proteins	Acyl carrier protein-like	ACP-like	Acyl-carrier protein (ACP)	Acyl carrier protein	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
F	d1f80f_	All alpha proteins	Acyl carrier protein-like	ACP-like	Acyl-carrier protein (ACP)	Acyl carrier protein	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
D	d1f80d1	All alpha proteins	Acyl carrier protein-like	ACP-like	Acyl-carrier protein (ACP)	Acyl carrier protein	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
D	d1f80d2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	4'-phosphopantetheinyl transferase	8032946	3000948	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	4'-phosphopantetheinyl transferase	8032946	3000948	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	4'-phosphopantetheinyl transferase	8032946	3000948	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	ACP-like	8032964	3001805	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	ACP-like	8032964	3001805	SCOP2B (2022-06-29)
D	SCOP2 Family	Acyl-carrier protein (ACP)	8020584	4000857	SCOP2 (2022-06-29)
D	SCOP2 Superfamily	ACP-like	8032964	3001805	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ACPS_1	e1f80A1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: ACPS_1	ECOD (1.6)
B	ACPS_1	e1f80B1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: ACPS_1	ECOD (1.6)
C	ACPS_1	e1f80C1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: ACPS_1	ECOD (1.6)
E	PP-binding	e1f80E1	A: alpha bundles	X: ACP-like	H: Acyl-carrier protein (ACP) (From Topology)	T: Acyl-carrier protein (ACP)	F: PP-binding	ECOD (1.6)
F	PP-binding	e1f80F1	A: alpha bundles	X: ACP-like	H: Acyl-carrier protein (ACP) (From Topology)	T: Acyl-carrier protein (ACP)	F: PP-binding	ECOD (1.6)
D	PP-binding	e1f80D1	A: alpha bundles	X: ACP-like	H: Acyl-carrier protein (ACP) (From Topology)	T: Acyl-carrier protein (ACP)	F: PP-binding	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.470.20	Alpha Beta	Alpha-Beta Complex	Ribosomal Protein L22	Chain A	CATH (4.3.0)
B	3.90.470.20	Alpha Beta	Alpha-Beta Complex	Ribosomal Protein L22	Chain A	CATH (4.3.0)
C	3.90.470.20	Alpha Beta	Alpha-Beta Complex	Ribosomal Protein L22	Chain A	CATH (4.3.0)
E	1.10.1200.10	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)
F	1.10.1200.10	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)
D	1.10.1200.10	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF01648	4'-phosphopantetheinyl transferase superfamily (ACPS)	4'-phosphopantetheinyl transferase superfamily	Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of Pfam:PF00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of ...	Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of Pfam:PF00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP [1]. This superfamily consists of two subtypes: The ACPS type such as Swiss:P24224 and the Sfp type such as Swiss:P39135. The structure of the Sfp type is known [3], which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.	Domain
D, E, F	PF00550	Phosphopantetheine attachment site (PP-binding)	Phosphopantetheine attachment site	A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes memb ...	A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	HOLO-(ACYL CARRIER PROTEIN) SYNTHASE	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding holo-[acyl-carrier-protein] synthase activity transferase activity catalytic activity phosphotransferase activity, for other substituted phosphate groups transferase activity, transferring phosphorus-containing groups	fatty acid metabolic process monocarboxylic acid biosynthetic process small molecule biosynthetic process oxoacid metabolic process carboxylic acid metabolic process lipid biosynthetic process primary metabolic process organic acid biosynthetic process organic acid metabolic process fatty acid biosynthetic process carboxylic acid biosynthetic process biosynthetic process small molecule metabolic process metabolic process fatty acid metabolic process monocarboxylic acid biosynthetic process small molecule biosynthetic process oxoacid metabolic process carboxylic acid metabolic process lipid biosynthetic process primary metabolic process organic acid biosynthetic process organic acid metabolic process fatty acid biosynthetic process carboxylic acid biosynthetic process biosynthetic process small molecule metabolic process metabolic process cellular process monocarboxylic acid metabolic process lipid metabolic process lysine metabolic process lysine biosynthetic process via aminoadipic acid aspartate family amino acid biosynthetic process aspartate family amino acid metabolic process amino acid metabolic process alpha-amino acid metabolic process lysine biosynthetic process L-amino acid biosynthetic process L-amino acid metabolic process proteinogenic amino acid metabolic process amino acid biosynthetic process proteinogenic amino acid biosynthetic process alpha-amino acid biosynthetic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol
D, E, F	ACYL CARRIER PROTEIN	phosphopantetheine-dependent carrier activity prosthetic group binding binding acyl carrier activity molecular carrier activity ACP phosphopantetheine attachment site binding acyl binding small molecule binding	glycolipid metabolic process lipid A biosynthetic process carbohydrate derivative metabolic process lipid biosynthetic process phospholipid metabolic process phosphate-containing compound metabolic process lipooligosaccharide biosynthetic process phosphorus metabolic process carbohydrate derivative biosynthetic process membrane lipid metabolic process lipooligosaccharide metabolic process liposaccharide metabolic process primary metabolic process glycolipid biosynthetic process glycolipid metabolic process lipid A biosynthetic process carbohydrate derivative metabolic process lipid biosynthetic process phospholipid metabolic process phosphate-containing compound metabolic process lipooligosaccharide biosynthetic process phosphorus metabolic process carbohydrate derivative biosynthetic process membrane lipid metabolic process lipooligosaccharide metabolic process liposaccharide metabolic process primary metabolic process glycolipid biosynthetic process phospholipid biosynthetic process lipid A metabolic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process metabolic process cellular process membrane lipid biosynthetic process lipid metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR037143	4'-phosphopantetheinyl transferase domain superfamily	Homologous Superfamily
A, B, C	IPR002582	Holo-[acyl carrier protein] synthase	Family
A, B, C	IPR050559	Phosphopantetheinyl Transferase Superfamily	Family
A, B, C	IPR004568	Phosphopantetheine-protein transferase domain	Domain
A, B, C	IPR008278	4'-phosphopantetheinyl transferase domain	Domain
D, E, F	IPR006162	Phosphopantetheine attachment site	PTM
D, E, F	IPR009081	Phosphopantetheine binding ACP domain	Domain
D, E, F	IPR036736	ACP-like superfamily	Homologous Superfamily
D, E, F	IPR003231	Acyl carrier protein	Family

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A, B, E	holo-[acyl-carrier-protein] synthase M-CSA #152	Holo-(acyl carrier protein) synthase (AcpS) from Bacillus subtilis is a member of the phosphopantetheinyl transferase superfamily. AcpS post-translationally modifies ACP to its holo form in order to activate it. AcpS catalyses the transfer of the 4'-phosphopantetheinyl (P-pant) moiety of coenzyme A to a serine residue on the ACP. This gives the activated ACP enzyme and Adenosine 3'5'-bisphosphate as products. This process is important as ACP enzymes play important roles in a number of biosynthetic pathways, such as the synthesis of fatty acids, vitamins, AcpS is essential in the initiation of the biosynthesis of fatty acids, polyketide antibiotics and non-ribosomal peptides.	Defined by 3 residues: LYS:A-61 [auth A-62]HIS:B-104 [auth B-105]ASP:E-40 [auth E-35] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.7.8.7 (PDB Primary Data) Search M-CSA Motif + EC 2.7.8.7

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.