1FPF

STRUCTURAL ASPECTS OF THE ALLOSTERIC INHIBITION OF FRUCTOSE-1,6-BISPHOSPHATASE BY AMP: THE BINDING OF BOTH THE SUBSTRATE ANALOGUE 2,5-ANHYDRO-D-GLUCITOL-1,6-BISPHOSPHATE AND CATALYTIC METAL IONS MONITORED BY X-RAY CRYSTALLOGRAPHY

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1fpfa_	Multi-domain proteins (alpha and beta)	Carbohydrate phosphatase	Carbohydrate phosphatase	Inositol monophosphatase/fructose-1,6-bisphosphatase-like	Fructose-1,6-bisphosphatase	pig (Sus scrofa ) [TaxId: 9823 ],	SCOPe (2.08)
B	d1fpfb_	Multi-domain proteins (alpha and beta)	Carbohydrate phosphatase	Carbohydrate phosphatase	Inositol monophosphatase/fructose-1,6-bisphosphatase-like	Fructose-1,6-bisphosphatase	pig (Sus scrofa ) [TaxId: 9823 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Carbohydrate phosphatase-like	8033633	3001721	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Carbohydrate phosphatase-like	8033633	3001721	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FBPase_N	e1fpfA1	A: a+b two layers	X: a+b domain in carbohydrate phosphatases (From Topology)	H: a+b domain in carbohydrate phosphatases (From Topology)	T: a+b domain in carbohydrate phosphatases	F: FBPase_N	ECOD (1.6)
A	FBPase_C	e1fpfA2	A: a/b three-layered sandwiches	X: Rossmann-like domain in carbohydrate phosphatases (From Homology)	H: Rossmann-like domain in carbohydrate phosphatases	T: Rossmann-like domain in carbohydrate phosphatases	F: FBPase_C	ECOD (1.6)
B	FBPase_N	e1fpfB1	A: a+b two layers	X: a+b domain in carbohydrate phosphatases (From Topology)	H: a+b domain in carbohydrate phosphatases (From Topology)	T: a+b domain in carbohydrate phosphatases	F: FBPase_N	ECOD (1.6)
B	FBPase_C	e1fpfB2	A: a/b three-layered sandwiches	X: Rossmann-like domain in carbohydrate phosphatases (From Homology)	H: Rossmann-like domain in carbohydrate phosphatases	T: Rossmann-like domain in carbohydrate phosphatases	F: FBPase_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.540.10	Alpha Beta	2-Layer Sandwich	Fructose-1,6-Bisphosphatase	Chain A, domain 1	CATH (4.3.0)
A	3.40.190.80	Alpha Beta	3-Layer(aba) Sandwich	D-Maltodextrin-Binding Protein	domain 2	CATH (4.3.0)
B	3.30.540.10	Alpha Beta	2-Layer Sandwich	Fructose-1,6-Bisphosphatase	Chain A, domain 1	CATH (4.3.0)
B	3.40.190.80	Alpha Beta	3-Layer(aba) Sandwich	D-Maltodextrin-Binding Protein	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00316	Fructose-1-6-bisphosphatase, N-terminal domain (FBPase)	Fructose-1-6-bisphosphatase, N-terminal domain	This family represents the N-terminus of this protein family.	Domain
A, B	PF18913	Fructose-1-6-bisphosphatase, C-terminal domain (FBPase_C)	Fructose-1-6-bisphosphatase, C-terminal domain	This entry represents the C-terminal domain of Fructose-1-6-bisphosphatase enzymes. According to ECOD this domain has a Rossmann-like fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	FRUCTOSE 1,6-BISPHOSPHATASE	phosphoric ester hydrolase activity fructose 1,6-bisphosphate 1-phosphatase activity hydrolase activity, acting on ester bonds carbohydrate phosphatase activity phosphatase activity hydrolase activity catalytic activity sugar-phosphatase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding cation binding AMP binding phosphoric ester hydrolase activity fructose 1,6-bisphosphate 1-phosphatase activity hydrolase activity, acting on ester bonds carbohydrate phosphatase activity phosphatase activity hydrolase activity catalytic activity sugar-phosphatase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding cation binding AMP binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding metal ion binding monosaccharide binding carbohydrate binding identical protein binding protein binding	organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process regulation of small molecule metabolic process small molecule metabolic process regulation of carbohydrate metabolic process organophosphate metabolic process cellular metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process carbohydrate derivative metabolic process metabolic process cellular process phosphorus metabolic process monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process regulation of small molecule metabolic process small molecule metabolic process regulation of carbohydrate metabolic process regulation of glucose metabolic process regulation of biosynthetic process carbohydrate metabolic process regulation of biological process small molecule biosynthetic process carbohydrate biosynthetic process biological regulation hexose metabolic process primary metabolic process monosaccharide biosynthetic process hexose biosynthetic process biosynthetic process regulation of gluconeogenesis regulation of carbohydrate biosynthetic process regulation of metabolic process glucose metabolic process sucrose biosynthetic process oligosaccharide metabolic process disaccharide metabolic process disaccharide biosynthetic process sucrose metabolic process oligosaccharide biosynthetic process cellular response to stimulus cellular response to metal ion cellular response to chemical stimulus response to stimulus response to metal ion response to chemical response to magnesium ion cellular response to magnesium ion fructose metabolic process fructose 1,6-bisphosphate metabolic process ribonucleotide catabolic process pyridine nucleotide catabolic process regulation of cellular process negative regulation of small molecule metabolic process negative regulation of glycolytic process oxoacid metabolic process negative regulation of biological process carbohydrate catabolic process organophosphate catabolic process regulation of generation of precursor metabolites and energy nucleoside phosphate catabolic process organic acid metabolic process negative regulation of ATP metabolic process negative regulation of cellular metabolic process regulation of ATP metabolic process nucleobase-containing compound metabolic process regulation of purine nucleotide catabolic process negative regulation of nucleotide metabolic process glycolytic process regulation of nucleotide metabolic process regulation of phosphate metabolic process pyridine-containing compound metabolic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process purine ribonucleotide catabolic process pyridine nucleotide metabolic process negative regulation of carbohydrate metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process pyruvate metabolic process regulation of carbohydrate catabolic process negative regulation of metabolic process negative regulation of purine nucleotide catabolic process purine nucleoside diphosphate metabolic process regulation of cellular metabolic process purine-containing compound catabolic process nucleotide metabolic process regulation of purine nucleotide metabolic process nucleobase-containing compound catabolic process nucleoside diphosphate metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process regulation of nucleotide catabolic process regulation of phosphorus metabolic process nucleoside triphosphate metabolic process monocarboxylic acid metabolic process purine ribonucleotide metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process catabolic process ATP metabolic process negative regulation of purine nucleotide metabolic process negative regulation of phosphate metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process negative regulation of cellular process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process negative regulation of nucleotide catabolic process negative regulation of catabolic process purine nucleoside triphosphate metabolic process purine nucleotide catabolic process carbohydrate derivative catabolic process ADP catabolic process carboxylic acid metabolic process regulation of catabolic process generation of precursor metabolites and energy regulation of glycolytic process purine-containing compound metabolic process ADP metabolic process negative regulation of phosphorus metabolic process purine ribonucleoside diphosphate metabolic process purine ribonucleoside diphosphate catabolic process cellular response to xenobiotic stimulus response to xenobiotic stimulus regulation of growth regulation of cellular component organization growth negative regulation of cell growth regulation of cell growth cell growth cellular component organization negative regulation of growth cellular component organization or biogenesis small GTPase-mediated signal transduction signaling negative regulation of signaling regulation of small GTPase mediated signal transduction negative regulation of intracellular signal transduction regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication regulation of intracellular signal transduction regulation of Ras protein signal transduction intracellular signaling cassette signal transduction regulation of signaling negative regulation of response to stimulus Ras protein signal transduction negative regulation of small GTPase mediated signal transduction negative regulation of signal transduction negative regulation of Ras protein signal transduction regulation of response to stimulus regulation of cell communication dephosphorylation	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR020548	Fructose-1,6-bisphosphatase, active site	Active Site
A, B	IPR033391	Fructose-1-6-bisphosphatase class I, N-terminal	Domain
A, B	IPR000146	Fructose-1,6-bisphosphatase class 1	Family
A, B	IPR028343	Fructose-1,6-bisphosphatase	Family
A, B	IPR044015	Fructose-1-6-bisphosphatase class 1, C-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.