1M7X

The X-ray Crystallographic Structure of Branching Enzyme

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
D	d1m7xd1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	E-set domains of sugar-utilizing enzymes	1,4-alpha-glucan branching enzyme, N-terminal domain N	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1m7xd2	All beta proteins	Glycosyl hydrolase domain	Glycosyl hydrolase domain	alpha-Amylases, C-terminal beta-sheet domain	1,4-alpha-glucan branching enzyme	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1m7xd3	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	Amylase, catalytic domain	1,4-alpha-glucan branching enzyme, central domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m7xa1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	E-set domains of sugar-utilizing enzymes	1,4-alpha-glucan branching enzyme, N-terminal domain N	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m7xa2	All beta proteins	Glycosyl hydrolase domain	Glycosyl hydrolase domain	alpha-Amylases, C-terminal beta-sheet domain	1,4-alpha-glucan branching enzyme	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1m7xa3	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	Amylase, catalytic domain	1,4-alpha-glucan branching enzyme, central domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1m7xb1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	E-set domains of sugar-utilizing enzymes	1,4-alpha-glucan branching enzyme, N-terminal domain N	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1m7xb2	All beta proteins	Glycosyl hydrolase domain	Glycosyl hydrolase domain	alpha-Amylases, C-terminal beta-sheet domain	1,4-alpha-glucan branching enzyme	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1m7xb3	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	Amylase, catalytic domain	1,4-alpha-glucan branching enzyme, central domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m7xc1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	E-set domains of sugar-utilizing enzymes	1,4-alpha-glucan branching enzyme, N-terminal domain N	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m7xc2	All beta proteins	Glycosyl hydrolase domain	Glycosyl hydrolase domain	alpha-Amylases, C-terminal beta-sheet domain	1,4-alpha-glucan branching enzyme	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1m7xc3	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	Amylase, catalytic domain	1,4-alpha-glucan branching enzyme, central domain	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D	SCOP2B Superfamily	(Trans)glycosidases	8043347	3000313	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	E set domains	8054943	3000070	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8043345	3001528	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8043345	3001528	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	E set domains	8054943	3000070	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	(Trans)glycosidases	8043347	3000313	SCOP2B (2022-06-29)
B	SCOP2 Family	alpha-Amylases, C-terminal beta-sheet domain	8030966	4002636	SCOP2 (2022-06-29)
B	SCOP2 Family	Amylase, catalytic domain	8030968	4003138	SCOP2 (2022-06-29)
B	SCOP2 Family	E-set domains of sugar-utilizing enzymes	8054942	4007565	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Glycosyl hydrolase domain-like	8043345	3001528	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	(Trans)glycosidases	8043347	3000313	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	E set domains	8054943	3000070	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	Glycosyl hydrolase domain-like	8043345	3001528	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	E set domains	8054943	3000070	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	(Trans)glycosidases	8043347	3000313	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D	AMPK1_CBM	e1m7xD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: AMPK1_CBM	ECOD (1.6)
D	Alpha-amylase_C	e1m7xD2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Alpha-amylase_C	ECOD (1.6)
D	Alpha-amylase	e1m7xD3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Alpha-amylase	ECOD (1.6)
A	AMPK1_CBM	e1m7xA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: AMPK1_CBM	ECOD (1.6)
A	Alpha-amylase_C	e1m7xA2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Alpha-amylase_C	ECOD (1.6)
A	Alpha-amylase	e1m7xA3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Alpha-amylase	ECOD (1.6)
B	AMPK1_CBM	e1m7xB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: AMPK1_CBM	ECOD (1.6)
B	Alpha-amylase_C	e1m7xB2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Alpha-amylase_C	ECOD (1.6)
B	Alpha-amylase	e1m7xB3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Alpha-amylase	ECOD (1.6)
C	AMPK1_CBM	e1m7xC5	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: AMPK1_CBM	ECOD (1.6)
C	Alpha-amylase_C	e1m7xC2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Alpha-amylase_C	ECOD (1.6)
C	Alpha-amylase	e1m7xC4	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Alpha-amylase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
D	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
D	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
A	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
A	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
B	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
B	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)
C	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
C	2.60.40.1180	Mainly Beta	Sandwich	Immunoglobulin-like	Golgi alpha-mannosidase II	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF02922	Carbohydrate-binding module 48 (Isoamylase N-terminal domain) (CBM_48)	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)	This domain is found in a range of enzymes that act on branched substrates - isoamylase, pullulanase and branching enzyme. This family also contains the beta subunit of 5' AMP activated kinase.	Domain
A, B, C, D	PF00128	Alpha amylase, catalytic domain (Alpha-amylase)	Alpha amylase, catalytic domain	Alpha amylase is classified as family 13 of the glycosyl hydrolases. The structure is an 8 stranded alpha/beta barrel containing the active site, interrupted by a ~70 a.a. calcium-binding domain protruding between beta strand 3 and alpha helix 3, a ...	Alpha amylase is classified as family 13 of the glycosyl hydrolases. The structure is an 8 stranded alpha/beta barrel containing the active site, interrupted by a ~70 a.a. calcium-binding domain protruding between beta strand 3 and alpha helix 3, and a carboxyl-terminal Greek key beta-barrel domain.	Domain
A, B, C, D	PF02806	Alpha amylase, C-terminal all-beta domain (Alpha-amylase_C)	Alpha amylase, C-terminal all-beta domain	Alpha amylase is classified as family 13 of the glycosyl hydrolases. The structure is an 8 stranded alpha/beta barrel containing the active site, interrupted by a ~70 a.a. calcium-binding domain protruding between beta strand 3 and alpha helix 3, a ...	Alpha amylase is classified as family 13 of the glycosyl hydrolases. The structure is an 8 stranded alpha/beta barrel containing the active site, interrupted by a ~70 a.a. calcium-binding domain protruding between beta strand 3 and alpha helix 3, and a carboxyl-terminal Greek key beta-barrel domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	1,4-alpha-glucan Branching Enzyme	1,4-alpha-glucan branching enzyme activity glycosyltransferase activity transferase activity hexosyltransferase activity catalytic activity cation binding ion binding binding small molecule binding hydrolase activity, acting on glycosyl bonds hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process carbohydrate metabolic process glucan biosynthetic process polysaccharide biosynthetic process energy derivation by oxidation of organic compounds carbohydrate biosynthetic process glycogen metabolic process primary metabolic process generation of precursor metabolites and energy cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process carbohydrate metabolic process glucan biosynthetic process polysaccharide biosynthetic process energy derivation by oxidation of organic compounds carbohydrate biosynthetic process glycogen metabolic process primary metabolic process generation of precursor metabolites and energy glycogen biosynthetic process macromolecule biosynthetic process polysaccharide metabolic process glucan metabolic process biosynthetic process metabolic process energy reserve metabolic process macromolecule metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR054169	1,4-alpha-glucan branching enzyme GlgB, N-terminal domain	Domain
A, B, C, D	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A, B, C, D	IPR006047	Glycosyl hydrolase, family 13, catalytic domain	Domain
A, B, C, D	IPR006048	Alpha-amylase/branching enzyme, C-terminal all beta	Domain
A, B, C, D	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
A, B, C, D	IPR014756	Immunoglobulin E-set	Homologous Superfamily
A, B, C, D	IPR044143	Glycogen branching enzyme GlgB, N-terminal Early set domain	Domain
A, B, C, D	IPR006407	1,4-alpha-glucan-branching enzyme, GlgB	Family
A, B, C, D	IPR013780	Glycosyl hydrolase, all-beta	Homologous Superfamily
A, B, C, D	IPR037439	1,4-alpha-glucan-branching enzyme	Family
A, B, C, D	IPR004193	Glycoside hydrolase, family 13, N-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.