1PYL

Crystal structure of Ribonuclease Sa2

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1pyla_	Alpha and beta proteins (a+b)	Microbial ribonucleases	Microbial ribonucleases	Bacterial ribonucleases	Ribonuclease Sa2	(Kitasatospora aureofaciens ) [TaxId: 1894 ],	SCOPe (2.08)
B	d1pylb_	Alpha and beta proteins (a+b)	Microbial ribonucleases	Microbial ribonucleases	Bacterial ribonucleases	Ribonuclease Sa2	(Kitasatospora aureofaciens ) [TaxId: 1894 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Bacterial ribonucleases	8025984	4001841	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Microbial ribonucleases	8038363	3001847	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Microbial ribonucleases	8038363	3001847	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Ribonuclease	e1pylA1	A: a+b two layers	X: Microbial ribonucleases-like	H: Microbial ribonucleases (From Topology)	T: Microbial ribonucleases	F: Ribonuclease	ECOD (1.6)
B	Ribonuclease	e1pylB1	A: a+b two layers	X: Microbial ribonucleases-like	H: Microbial ribonucleases (From Topology)	T: Microbial ribonucleases	F: Ribonuclease	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.450.30	Alpha Beta	Roll	Nuclear Transport Factor 2	Chain: A,	CATH (4.3.0)
B	3.10.450.30	Alpha Beta	Roll	Nuclear Transport Factor 2	Chain: A,	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	ribonuclease	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR000026	Guanine-specific ribonuclease N1-like	Family
A, B	IPR016191	Ribonuclease/ribotoxin	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	guanyl-specific ribonuclease Sa M-CSA #459	Three different strains of Streptomyces aureofaciens produce the homologous ribonucleases Sa, Sa2 and Sa3. They are all guanylate endoribonucleases, highly specifically hydrolysing the phosphodiester bonds of RNA at the 3'-side of guanosine nucleotides. These enzymes belong to the prokaryotic subgroup of microbial ribonucleases, and are some of the smallest enzymes known.	Defined by 4 residues: GLU:A-56ARG:A-67ARG:A-71HIS:A-86 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.4.8 (UniProt) Search M-CSA Motif + EC 3.1.4.8

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.