1S9C

Crystal structure analysis of the 2-enoyl-CoA hydratase 2 domain of human peroxisomal multifunctional enzyme type 2

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1s9ca1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1s9cb1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d1s9cc1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d1s9cd1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F	d1s9cf1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
G	d1s9cg1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
H	d1s9ch1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
I	d1s9ci1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
K	d1s9ck1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E	d1s9ce1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
J	d1s9cj1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
L	d1s9cl1	Alpha and beta proteins (a+b)	Thioesterase/thiol ester dehydrase-isomerase	Thioesterase/thiol ester dehydrase-isomerase	MaoC-like	2-enoyl-coa hydratase domain of multifunctional peroxisomal hydratase-dehydrogenase-epimerase	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
G	SCOP2 Family	MaoC-like	8003949	4000361	SCOP2 (2022-06-29)
G	SCOP2 Family	MaoC-like	8003952	4000361	SCOP2 (2022-06-29)
G	SCOP2 Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2 (2022-06-29)
G	SCOP2 Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2 (2022-06-29)
H	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8003950	3000149	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8091656	3000149	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	MaoC_dehydratas	e1s9cA3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
A	MaoC_dehydrat_N	e1s9cA2	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
B	MaoC_dehydratas	e1s9cB2	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
B	MaoC_dehydrat_N	e1s9cB1	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
C	MaoC_dehydratas	e1s9cC2	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
C	MaoC_dehydrat_N	e1s9cC3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
D	MaoC_dehydratas	e1s9cD4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
D	MaoC_dehydrat_N	e1s9cD3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
F	MaoC_dehydratas	e1s9cF3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
F	MaoC_dehydrat_N	e1s9cF4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
G	MaoC_dehydratas	e1s9cG3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
G	MaoC_dehydrat_N	e1s9cG4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
H	MaoC_dehydratas	e1s9cH3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
H	MaoC_dehydrat_N	e1s9cH4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
I	MaoC_dehydratas	e1s9cI3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
I	MaoC_dehydrat_N	e1s9cI4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
K	MaoC_dehydratas	e1s9cK4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
K	MaoC_dehydrat_N	e1s9cK3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
E	MaoC_dehydratas	e1s9cE1	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
E	MaoC_dehydrat_N	e1s9cE2	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
J	MaoC_dehydratas	e1s9cJ4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
J	MaoC_dehydrat_N	e1s9cJ3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)
L	MaoC_dehydratas	e1s9cL4	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydratas	ECOD (1.6)
L	MaoC_dehydrat_N	e1s9cL3	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: MaoC_dehydrat_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
B	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
C	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
D	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
F	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
G	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
H	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
I	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
K	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
E	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
J	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
L	3.10.129.10	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF22622	MFE-2 hydratase 2 N-terminal domain (MFE-2_hydrat-2_N)	MFE-2 hydratase 2 N-terminal domain	This entry represents a domain found in N-terminal in MFE-2 hydratase 2 and related enzymes. This domain is housing the cavity for the aliphatic acyl part of the substrate molecule. The flexibility of region within this domain plays a role in substra ...	This entry represents a domain found in N-terminal in MFE-2 hydratase 2 and related enzymes. This domain is housing the cavity for the aliphatic acyl part of the substrate molecule. The flexibility of region within this domain plays a role in substrate binding in mammalian hydratase 2 [2].	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF01575	MaoC like domain (MaoC_dehydratas)	MaoC like domain	The maoC gene is part of a operon with maoA which is involved in the synthesis of monoamine oxidase [1]. The MaoC protein is found to share similarity with a wide variety of enzymes; estradiol 17 beta-dehydrogenase 4, peroxisomal hydratase-dehydroge ...	The maoC gene is part of a operon with maoA which is involved in the synthesis of monoamine oxidase [1]. The MaoC protein is found to share similarity with a wide variety of enzymes; estradiol 17 beta-dehydrogenase 4, peroxisomal hydratase-dehydrogenase-epimerase, fatty acid synthase beta subunit. Several bacterial proteins that are composed solely of this domain have (R)-specific enoyl-CoA hydratase activity [2]. This domain is also present in the NodN nodulation protein N.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	Peroxisomal multifunctional enzyme type 2	oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors (3R)-hydroxyacyl-CoA dehydrogenase (NAD+) activity catalytic activity enoyl-CoA hydratase activity hydro-lyase activity 3-hydroxyacyl-CoA dehydratase activity lyase activity carbon-oxygen lyase activity 3-hydroxyacyl-CoA dehydrogenase activity steroid dehydrogenase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor 17-beta-hydroxysteroid dehydrogenase (NAD+) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors (3R)-hydroxyacyl-CoA dehydrogenase (NAD+) activity catalytic activity enoyl-CoA hydratase activity hydro-lyase activity 3-hydroxyacyl-CoA dehydratase activity lyase activity carbon-oxygen lyase activity 3-hydroxyacyl-CoA dehydrogenase activity steroid dehydrogenase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor 17-beta-hydroxysteroid dehydrogenase (NAD+) activity 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity isomerase activity estradiol 17-beta-dehydrogenase [NAD(P)+] activity	developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation nucleoside phosphate metabolic process very long-chain fatty-acyl-CoA metabolic process primary metabolic process purine-containing compound metabolic process fatty acid derivative metabolic process nucleobase-containing compound metabolic process acyl-CoA metabolic process developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation nucleoside phosphate metabolic process very long-chain fatty-acyl-CoA metabolic process primary metabolic process purine-containing compound metabolic process fatty acid derivative metabolic process nucleobase-containing compound metabolic process acyl-CoA metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process metabolic process sulfur compound metabolic process small molecule metabolic process fatty-acyl-CoA metabolic process amide metabolic process thioester metabolic process phosphorus metabolic process lipid metabolic process regulation of hormone levels steroid metabolic process biological regulation androgen metabolic process regulation of biological quality hormone metabolic process fatty acid metabolic process lipid catabolic process catabolic process oxoacid metabolic process carboxylic acid metabolic process lipid modification organic acid catabolic process organic acid metabolic process fatty acid catabolic process cellular catabolic process monocarboxylic acid catabolic process fatty acid oxidation carboxylic acid catabolic process fatty acid beta-oxidation monocarboxylic acid metabolic process lipid oxidation small molecule catabolic process estrogen metabolic process very long-chain fatty acid metabolic process reproductive process sex differentiation cell development developmental process involved in reproduction epithelial cell differentiation Sertoli cell differentiation male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development epithelium development gonad development reproductive system development Sertoli cell development animal organ development tissue development epithelial cell development medium-chain fatty-acyl-CoA metabolic process	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle peroxisome microbody intracellular membrane-bounded organelle microbody membrane bounding membrane of organelle peroxisomal membrane cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle peroxisome microbody intracellular membrane-bounded organelle microbody membrane bounding membrane of organelle peroxisomal membrane organelle membrane peroxisomal matrix membrane-enclosed lumen intracellular organelle lumen organelle lumen microbody lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR051687	Peroxisomal Beta-Oxidation Enzymes	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR002347	Short-chain dehydrogenase/reductase SDR	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR020904	Short-chain dehydrogenase/reductase, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR036527	SCP2 sterol-binding domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR003033	SCP2 sterol-binding domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR029069	HotDog domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR002539	MaoC-like dehydratase domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR054357	Peroxisomal multifunctional enzyme type 2-like, N-terminal domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	Pharos: P51659	d-bifunctional protein deficiency Perrault syndrome Zellweger spectrum disorders peroxisomal disease West syndrome Perrault syndrome 1 Peroxisomal Dysfunction, Single polycystic ovary syndrome Spasmus nutans developmental and epileptic encephalopathy lymphoplasmacytic lymphoma ovarian cancer allergic rhinitis leukodystrophy pancreatic ductal adenocarcinoma d-bifunctional protein deficiency Perrault syndrome Zellweger spectrum disorders peroxisomal disease West syndrome Perrault syndrome 1 Peroxisomal Dysfunction, Single polycystic ovary syndrome Spasmus nutans developmental and epileptic encephalopathy lymphoplasmacytic lymphoma ovarian cancer allergic rhinitis leukodystrophy pancreatic ductal adenocarcinoma tuberculosis dermatomyositis intellectual disability lung cancer

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	enoyl-CoA hydratase M-CSA #461	Mammalian peroxisomal multifunctional enzyme type 2 (MFE-2) is important in the beta-oxidation of very-long-chain and alpha-methyl-branched fatty acids as well as the synthesis of bile acids. It has three units: a dehydrogenase unit, an sterol carrier protein (SCP)-like unit and a central hydratase unit. This entry represents the hydratase function, which reversibly catalyses the addition of water to the beta-carbon of trans-2-enoyl-CoAs with (R) specificity (the opposite to MFE-1).	Defined by 4 residues: ASP:A-193HIS:A-198ILE:A-213GLY:A-216 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 4.2.1 (PDB Primary Data) Search M-CSA Motif + EC 4.2.1

Prepare Data

Validate Data

Deposit Data

Help and Resources

1S9C

Crystal structure analysis of the 2-enoyl-CoA hydratase 2 domain of human peroxisomal multifunctional enzyme type 2

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Domain Annotation: ECOD Classification ECOD Database Homepage

Domain Annotation: CATH CATH Database Homepage

Protein Family Annotation Pfam Database Homepage

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

InterPro: Protein Family Classification InterPro Database Homepage

Pharos: Disease Associations Pharos Homepage Annotation

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage