1U46

Crystal Structure of the Unphosphorylated Kinase Domain of the Tyrosine Kinase ACK1

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1u46a_	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	Protein kinases, catalytic subunit	Activated CDC42 kinase 1, ACK1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1u46b_	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	Protein kinases, catalytic subunit	Activated CDC42 kinase 1, ACK1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Protein kinase-like (PK-like)	8040719	3000066	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Protein kinase-like (PK-like)	8040719	3000066	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Pkinase_Tyr	e1u46A1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
B	Pkinase_Tyr	e1u46B1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
B	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
B	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF07714	Protein tyrosine and serine/threonine kinase (PK_Tyr_Ser-Thr)	Protein tyrosine and serine/threonine kinase	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosph ...	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. Phosphoprotein phosphatases catalyse the reverse process. Protein kinases fall into three broad classes, characterised with respect to substrate specificity [1]; Serine/threonine-protein kinases, tyrosine-protein kinases, and dual specificity protein kinases (e.g. MEK - phosphorylates both Thr and Tyr on target proteins). This entry represents the catalytic domain found in a number of serine/threonine- and tyrosine-protein kinases. It does not include the catalytic domain of dual specificity kinases.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Activated CDC42 kinase 1	protein serine/threonine/tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein binding binding WW domain binding protein domain specific binding protein serine kinase activity protein tyrosine kinase activity protein serine/threonine/tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein binding binding WW domain binding protein domain specific binding protein serine kinase activity protein tyrosine kinase activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding GTPase regulator activity enzyme regulator activity GTPase inhibitor activity molecular function inhibitor activity nucleoside-triphosphatase regulator activity molecular function regulator activity enzyme inhibitor activity non-membrane spanning protein tyrosine kinase activity protein serine/threonine kinase activity epidermal growth factor receptor binding signaling receptor binding growth factor receptor binding ubiquitin protein ligase binding ubiquitin-like protein ligase binding enzyme binding cation binding metal ion binding identical protein binding	localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport regulation of localization regulation of biological process regulation of cellular process regulation of cellular component organization regulation of transport regulation of receptor-mediated endocytosis biological regulation localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport regulation of localization regulation of biological process regulation of cellular process regulation of cellular component organization regulation of transport regulation of receptor-mediated endocytosis biological regulation regulation of vesicle-mediated transport cellular component organization clathrin-dependent endocytosis cellular component organization or biogenesis receptor-mediated endocytosis regulation of clathrin-dependent endocytosis regulation of endocytosis cellular response to stimulus cell surface receptor signaling pathway response to stimulus signaling signal transduction cell communication phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process phosphorus metabolic process small GTPase-mediated signal transduction intracellular signal transduction intracellular signaling cassette regulation of protein modification process positive regulation of phosphorylation regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of biological process positive regulation of cellular metabolic process peptidyl-tyrosine modification positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein modification process regulation of phosphate metabolic process peptidyl-amino acid modification positive regulation of macromolecule metabolic process protein metabolic process protein modification process positive regulation of protein phosphorylation positive regulation of cellular process protein phosphorylation positive regulation of phosphorus metabolic process regulation of protein metabolic process regulation of peptidyl-tyrosine phosphorylation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process macromolecule modification regulation of phosphorylation regulation of phosphorus metabolic process macromolecule metabolic process peptidyl-tyrosine phosphorylation regulation of metabolic process	cytoophidium supramolecular fiber cellular anatomical entity supramolecular polymer supramolecular complex clathrin-coated pit membrane endomembrane system cell periphery protein-containing complex Grb2-EGFR complex plasma membrane protein complex plasma membrane membrane protein complex cytoophidium supramolecular fiber cellular anatomical entity supramolecular polymer supramolecular complex clathrin-coated pit membrane endomembrane system cell periphery protein-containing complex Grb2-EGFR complex plasma membrane protein complex plasma membrane membrane protein complex membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle clathrin-coated vesicle coated vesicle cytoplasm vesicle cytosol cytoplasmic vesicle membrane vesicle membrane organelle membrane cell-cell junction adherens junction anchoring junction cell junction endosome perinuclear region of cytoplasm nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR015116	Cdc42 binding domain-like	Domain
A, B	IPR017441	Protein kinase, ATP binding site	Binding Site
A, B	IPR052112	EGFR Signaling Regulator Kinase	Family
A, B	IPR008266	Tyrosine-protein kinase, active site	Active Site
A, B	IPR020635	Tyrosine-protein kinase, catalytic domain	Domain
A, B	IPR036028	SH3-like domain superfamily	Homologous Superfamily
A, B	IPR000719	Protein kinase domain	Domain
A, B	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	Domain
A, B	IPR021619	Mig-6 domain	Domain
A, B	IPR037085	Cdc42 binding domain-like superfamily	Homologous Superfamily
A, B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B	IPR001452	SH3 domain	Domain
A, B	IPR049587	TNK(ACK)-like non-receptor tyrosine-protein kinases, SAM domain	Domain
A, B	IPR030220	Activated CDC42 kinase 1, UBA domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: Q07912	liver cancer ALK fusion gene-positive non-small-cell lung cancer malignant ependymoma oligodendroglioma ovarian mucinous adenocarcinoma atypical teratoid rhabdoid tumor gastric adenocarcinoma liver neoplasm medulloblastoma osteosarcoma precancerous condition large cell medulloblastoma lung adenocarcinoma non-small cell lung carcinoma ovarian endometrioid adenocarcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.