1Z9H

Microsomal prostaglandin E synthase type-2

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1z9ha1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
A	d1z9ha2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
B	d1z9hb1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
B	d1z9hb2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
C	d1z9hc1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
C	d1z9hc2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
D	d1z9hd1	All alpha proteins	GST C-terminal domain-like	GST C-terminal domain-like	Glutathione S-transferase (GST), C-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)
D	d1z9hd2	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Glutathione S-transferase (GST), N-terminal domain	Microsomal prostaglandin E synthase-2	crab-eating macaque (Macaca fascicularis ) [TaxId: 9541 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Glutathione S-transferase (GST), C-terminal domain	8023062	4000976	SCOP2 (2022-06-29)
A	SCOP2 Family	Glutathione S-transferase (GST), N-terminal domain	8023064	4000472	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	GST C-terminal domain-like	8035442	3000305	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thioredoxin-like	8035444	3000031	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	GST C-terminal domain-like	8035442	3000305	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thioredoxin-like	8035444	3000031	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Thioredoxin-like	8035444	3000031	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	GST C-terminal domain-like	8035442	3000305	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	GST C-terminal domain-like	8035442	3000305	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Thioredoxin-like	8035444	3000031	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	GST_C_2	e1z9hA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_2	ECOD (1.6)
A	Glutaredoxin	e1z9hA2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: Glutaredoxin	ECOD (1.6)
B	GST_C_2	e1z9hB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_2	ECOD (1.6)
B	Glutaredoxin	e1z9hB2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: Glutaredoxin	ECOD (1.6)
C	GST_C_2	e1z9hC1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_2	ECOD (1.6)
C	Glutaredoxin	e1z9hC2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: Glutaredoxin	ECOD (1.6)
D	GST_C_2	e1z9hD1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Glutathione S-transferase (GST)-C (From Topology)	T: Glutathione S-transferase (GST)-C	F: GST_C_2	ECOD (1.6)
D	Glutaredoxin	e1z9hD2	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: Glutaredoxin	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
A	6.20.200.30	Special	Other non-globular	Defensin A-like		CATH (4.3.0)
A	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
B	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
B	6.20.200.30	Special	Other non-globular	Defensin A-like		CATH (4.3.0)
B	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
C	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
C	6.20.200.30	Special	Other non-globular	Defensin A-like		CATH (4.3.0)
C	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)
D	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
D	6.20.200.30	Special	Other non-globular	Defensin A-like		CATH (4.3.0)
D	1.20.1050.10	Mainly Alpha	Up-down Bundle	Glutathione S-transferase Yfyf (Class Pi)	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF13417	Glutathione S-transferase, N-terminal domain (GST_N_3)	Glutathione S-transferase, N-terminal domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	membrane-associated prostaglandin E synthase-2	oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors 12-hydroxyheptadecatrienoic acid synthase activity catalytic activity binding organic cyclic compound binding tetrapyrrole binding heme binding amide binding modified amino acid binding ion binding oligopeptide binding anion binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors 12-hydroxyheptadecatrienoic acid synthase activity catalytic activity binding organic cyclic compound binding tetrapyrrole binding heme binding amide binding modified amino acid binding ion binding oligopeptide binding anion binding glutathione binding sulfur compound binding small molecule binding lyase activity isomerase activity intramolecular oxidoreductase activity prostaglandin-E synthase activity identical protein binding protein binding	icosanoid metabolic process prostaglandin metabolic process unsaturated fatty acid metabolic process oxoacid metabolic process cellular biosynthetic process lipid biosynthetic process organic acid biosynthetic process organic acid metabolic process carboxylic acid biosynthetic process prostanoid metabolic process icosanoid biosynthetic process small molecule metabolic process prostaglandin biosynthetic process prostanoid biosynthetic process icosanoid metabolic process prostaglandin metabolic process unsaturated fatty acid metabolic process oxoacid metabolic process cellular biosynthetic process lipid biosynthetic process organic acid biosynthetic process organic acid metabolic process carboxylic acid biosynthetic process prostanoid metabolic process icosanoid biosynthetic process small molecule metabolic process prostaglandin biosynthetic process prostanoid biosynthetic process fatty acid metabolic process monocarboxylic acid biosynthetic process small molecule biosynthetic process cellular metabolic process carboxylic acid metabolic process primary metabolic process fatty acid biosynthetic process unsaturated fatty acid biosynthetic process biosynthetic process cellular lipid metabolic process metabolic process cellular process monocarboxylic acid metabolic process lipid metabolic process	membrane-bounded organelle Golgi membrane intracellular anatomical structure Golgi apparatus membrane cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle bounding membrane of organelle organelle cytoplasm organelle membrane perinuclear region of cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR010987	Glutathione S-transferase, C-terminal-like	Domain
A, B, C, D	IPR004045	Glutathione S-transferase, N-terminal	Domain
A, B, C, D	IPR034334	Prostaglandin E synthase 2	Family
A, B, C, D	IPR036282	Glutathione S-transferase, C-terminal domain superfamily	Homologous Superfamily
A, B, C, D	IPR034335	Prostaglandin E synthase 2, C-terminal	Domain
A, B, C, D	IPR036249	Thioredoxin-like superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D	OPM	Monotopic/peripheral	Alpha/Beta monotopic/peripheral	Thioredoxin-like	Glutathione S-transferase

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	prostaglandin-E synthase M-CSA #192	Microsomal Prostaglandin E Synthase Type 2 (mPGES-2), sourced from Macaca fascicularis catalyses the isomerization reaction of PGH2 (Prostaglandin H2) to PGE2 (Prostaglandin E2). PGH2 is formed from arachidonic acid. It is an unstable intermediate and so is converted into PGE2. PGE2 exerts control over various biological activities such as relaxation/ contraction of smooth muscle, excretion of Na+, body temperature and the physiological sleep/ wake cycle. The enzyme is active in the absence of an R-SH reagent, but the catalytic activity is increased by the presence of an R-SH reagent, suggesting that a water molecule and the SH group of an R-SH bind the same site and participate in the same catalytic role. An R-SH or water molecule bound between O-eta of Tyr107 and C9 of PGH2 is polarised by forming a H-bond with Tyr107, and consequently, the SH group of R-SH or water is deprotonated at neutral pH.	Defined by 4 residues: TYR:A-20 [auth A-107]CYS:A-23 [auth A-110]PHE:A-25 [auth A-112]CYS:A-26 [auth A-113] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 5.3.99.3 (PDB Primary Data) Search M-CSA Motif + EC 5.3.99.3

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.