1ZXE

Crystal Structure of eIF2alpha Protein Kinase GCN2: D835N Inactivating Mutant in Apo Form

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C	d1zxec1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
C	d1zxec2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
A	d1zxea1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
A	d1zxea2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d1zxeb1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d1zxeb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
D	d1zxed1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
D	d1zxed2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
E	d1zxee1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
E	d1zxee2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
F	d1zxef1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
F	d1zxef2	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ], baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	Pkinase_Tyr	e1zxeC2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
A	Pkinase_Tyr	e1zxeA2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
B	Pkinase_Tyr	e1zxeB1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
D	Pkinase_Tyr	e1zxeD2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
E	Pkinase_Tyr	e1zxeE1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
F	Pkinase_Tyr	e1zxeF1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
C	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
B	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
B	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
D	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
D	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
E	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
E	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
F	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
F	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Serine/threonine-protein kinase	enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity kinase inhibitor activity kinase regulator activity RNA binding double-stranded RNA binding binding organic cyclic compound binding nucleic acid binding protein serine/threonine kinase activity enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity kinase inhibitor activity kinase regulator activity RNA binding double-stranded RNA binding binding organic cyclic compound binding nucleic acid binding protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups tRNA binding protein serine kinase activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding identical protein binding protein binding protein homodimerization activity protein dimerization activity translation initiation factor binding ribonucleoprotein complex binding protein-containing complex binding ribosomal large subunit binding ribosome binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation integrated stress response signaling intracellular signal transduction cell communication cellular response to stress response to stress intracellular signaling cassette cellular process signal transduction cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation integrated stress response signaling intracellular signal transduction cell communication cellular response to stress response to stress intracellular signaling cassette cellular process signal transduction GCN2-mediated signaling positive regulation of cellular response to amino acid starvation response to nutrient levels regulation of response to nutrient levels positive regulation of biological process regulation of cellular response to stress cellular response to amino acid starvation positive regulation of response to stimulus regulation of response to stress response to amino acid starvation response to starvation positive regulation of cellular process cellular response to starvation cellular response to nutrient levels regulation of response to stimulus regulation of cellular response to amino acid starvation positive regulation of response to nutrient levels protein phosphorylation protein autophosphorylation phosphorylation cellular metabolic process primary metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process cellular response to acid chemical cellular response to nitrogen compound cellular response to amino acid stimulus cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound response to amino acid cellular response to oxygen-containing compound cellular response to histidine response to acid chemical cellular response to chemical stimulus response to histidine response to chemical response to organic cyclic compound cellular biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression translational initiation regulation of biosynthetic process regulation of translational initiation regulation of protein metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression translation macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of translation regulation of macromolecule biosynthetic process regulation of metabolic process negative regulation of translation in response to stress negative regulation of biological process regulation of cytoplasmic translational initiation in response to stress negative regulation of cellular metabolic process negative regulation of protein metabolic process negative regulation of metabolic process negative regulation of cellular biosynthetic process negative regulation of gene expression negative regulation of cytoplasmic translational initiation in response to stress negative regulation of macromolecule biosynthetic process regulation of cytoplasmic translation negative regulation of translation regulation of translation in response to stress negative regulation of cellular process negative regulation of cytoplasmic translational initiation negative regulation of macromolecule metabolic process negative regulation of translational initiation in response to stress regulation of cytoplasmic translation in response to stress negative regulation of translational initiation negative regulation of biosynthetic process regulation of cytoplasmic translational initiation cytoplasmic translation regulation of translational initiation in response to stress cytoplasmic translational initiation positive regulation of macromolecule biosynthetic process positive regulation of translational initiation in response to stress positive regulation of metabolic process positive regulation of macromolecule metabolic process positive regulation of translation in response to stress positive regulation of translational initiation positive regulation of protein metabolic process positive regulation of translation positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of translational initiation in response to starvation positive regulation of gene expression regulation of translational initiation in response to starvation positive regulation of cellular biosynthetic process DNA damage checkpoint signaling regulation of cell cycle phase transition negative regulation of cell cycle cell cycle phase transition negative regulation of cell cycle phase transition cell cycle regulation of cell cycle cell cycle process regulation of cell cycle process DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling DNA damage response signal transduction in response to DNA damage	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol protein-containing complex small ribosomal subunit ribosomal subunit intracellular organelle organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle cytosolic ribosome intracellular anatomical structure cytoplasm cellular anatomical entity cytosol protein-containing complex small ribosomal subunit ribosomal subunit intracellular organelle organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle cytosolic ribosome large ribosomal subunit membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR017441	Protein kinase, ATP binding site	Binding Site
A, B, C, D, E A, B, C, D, E, F	IPR045864	Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL)	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR016135	Ubiquitin-conjugating enzyme/RWD-like	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR050339	Cell Cycle and Stress Response Kinase	Family
A, B, C, D, E A, B, C, D, E, F	IPR000719	Protein kinase domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR006575	RWD domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR036621	Anticodon-binding domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR016255	eIF-2-alpha kinase Gcn2	Family
A, B, C, D, E A, B, C, D, E, F	IPR024435	Histidyl tRNA synthetase-related domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR008271	Serine/threonine-protein kinase, active site	Active Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.