1MPX

ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1mpxa1	All beta proteins	Galactose-binding domain-like	Galactose-binding domain-like	PepX C-terminal domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
A	d1mpxa2	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	PepX catalytic domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
B	d1mpxb1	All beta proteins	Galactose-binding domain-like	Galactose-binding domain-like	PepX C-terminal domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
B	d1mpxb2	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	PepX catalytic domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
C	d1mpxc1	All beta proteins	Galactose-binding domain-like	Galactose-binding domain-like	PepX C-terminal domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
C	d1mpxc2	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	PepX catalytic domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
D	d1mpxd1	All beta proteins	Galactose-binding domain-like	Galactose-binding domain-like	PepX C-terminal domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)
D	d1mpxd2	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	PepX catalytic domain-like	Alpha-amino acid ester hydrolase	(Xanthomonas citri ) [TaxId: 346 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	pepx c-terminal domain-like	8020445	4003032	SCOP2 (2022-06-29)
A	SCOP2 Family	PepX catalytic domain-like	8020446	4003340	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	galactose-binding domain-like	8032825	3000087	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	alpha/beta-Hydrolases	8032826	3000102	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	alpha/beta-Hydrolases	8032826	3000102	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	galactose-binding domain-like	8032825	3000087	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	galactose-binding domain-like	8032825	3000087	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	alpha/beta-Hydrolases	8032826	3000102	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	alpha/beta-Hydrolases	8032826	3000102	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	galactose-binding domain-like	8032825	3000087	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PepX_C	e1mpxA1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: PepX_C	ECOD (1.6)
A	Hydrolase_4	e1mpxA2	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: Hydrolase_4	ECOD (1.6)
B	PepX_C	e1mpxB1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: PepX_C	ECOD (1.6)
B	Hydrolase_4	e1mpxB2	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: Hydrolase_4	ECOD (1.6)
C	PepX_C	e1mpxC1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: PepX_C	ECOD (1.6)
C	Hydrolase_4	e1mpxC2	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: Hydrolase_4	ECOD (1.6)
D	PepX_C	e1mpxD1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: PepX_C	ECOD (1.6)
D	Hydrolase_4	e1mpxD2	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: Hydrolase_4	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
A	1.10.3020.10	Mainly Alpha	Orthogonal Bundle	alpha-amino acid ester hydrolase ( Helical cap domain)	alpha-amino acid ester hydrolase ( Helical cap domain)	CATH (4.3.0)
A	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)
B	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
B	1.10.3020.10	Mainly Alpha	Orthogonal Bundle	alpha-amino acid ester hydrolase ( Helical cap domain)	alpha-amino acid ester hydrolase ( Helical cap domain)	CATH (4.3.0)
B	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)
C	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
C	1.10.3020.10	Mainly Alpha	Orthogonal Bundle	alpha-amino acid ester hydrolase ( Helical cap domain)	alpha-amino acid ester hydrolase ( Helical cap domain)	CATH (4.3.0)
C	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)
D	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
D	1.10.3020.10	Mainly Alpha	Orthogonal Bundle	alpha-amino acid ester hydrolase ( Helical cap domain)	alpha-amino acid ester hydrolase ( Helical cap domain)	CATH (4.3.0)
D	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF08530	X-Pro dipeptidyl-peptidase C-terminal non-catalytic domain (PepX_C)	X-Pro dipeptidyl-peptidase C-terminal non-catalytic domain	This domain contains a beta sandwich domain.	Domain
A, B, C, D	PF02129	X-Pro dipeptidyl-peptidase (S15 family) (Peptidase_S15)	X-Pro dipeptidyl-peptidase (S15 family)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	alpha-amino acid ester hydrolase	peptidase activity catalytic activity, acting on a protein dipeptidyl-peptidase activity hydrolase activity exopeptidase activity catalytic activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity alpha-amino-acid esterase activity cation binding ion binding binding metal ion binding small molecule binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR005674	CocE/Serine esterase	Family
A, B, C, D	IPR050585	Xaa-Pro dipeptidyl-peptidase/Cocaine esterase	Family
A, B, C, D	IPR000383	Xaa-Pro dipeptidyl-peptidase-like domain	Domain
A, B, C, D	IPR013736	Xaa-Pro dipeptidyl-peptidase, C-terminal	Domain
A, B, C, D	IPR008979	Galactose-binding-like domain superfamily	Homologous Superfamily
A, B, C, D	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	alpha-amino-acid esterase M-CSA #666	alpha-amino acid ester hydrolases (AEHs) catalyse the hydrolysis and synthesis of esters and amides with an alpha-amino group. They can be used to acylate a beta-lactam using an ester as acyl donor without cleaving the beta-lactam nuclei, so the enzyme is suitable for generating widely used antibiotics such as ampicillin, amoxicillin, and the cephalosporins, cephadroxil and cephalexin.	Defined by 5 residues: TYR:A-60 [auth A-82]SER:A-152 [auth A-174]TYR:A-153 [auth A-175]ASP:A-285 [auth A-307]HIS:A-318 [auth A-340] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.1.43 (PDB Primary Data) Search M-CSA Motif + EC 3.1.1.43

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.