2BRF

Crystal Structure of the FHA Domain of Human Polynucleotide Kinase 3' Phosphatase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2brfa1	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	FHA domain	Polynucleotide kinase 3'-phosphatase	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	FHA domain	8019140	4002605	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	SMAD/FHA domain-like	8058041	3000137	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	FHA_1	e2brfA1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: FHA_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF17913	FHA domain (FHA_2)	FHA domain	This entry represents a divergent FHA domain which in PNK binds to phosphorylated segment of XRCC1 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE	DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding nucleobase-containing compound kinase activity polynucleotide 5'-hydroxyl-kinase activity kinase activity ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity transferase activity ATP-dependent polynucleotide 5'-hydroxyl-kinase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding nucleobase-containing compound kinase activity polynucleotide 5'-hydroxyl-kinase activity kinase activity ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity transferase activity ATP-dependent polynucleotide 5'-hydroxyl-kinase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding double-stranded DNA binding phosphoric ester hydrolase activity hydrolase activity, acting on ester bonds phosphatase activity hydrolase activity polynucleotide 3'-phosphatase activity endonuclease activity nuclease activity	regulation of molecular function regulation of catalytic activity negative regulation of transferase activity biological regulation negative regulation of molecular function negative regulation of catalytic activity regulation of transferase activity negative regulation of protein ADP-ribosylation regulation of protein ADP-ribosylation cellular response to stimulus regulation of cellular process regulation of double-strand break repair response to stimulus regulation of cellular response to stress regulation of molecular function regulation of catalytic activity negative regulation of transferase activity biological regulation negative regulation of molecular function negative regulation of catalytic activity regulation of transferase activity negative regulation of protein ADP-ribosylation regulation of protein ADP-ribosylation cellular response to stimulus regulation of cellular process regulation of double-strand break repair response to stimulus regulation of cellular response to stress positive regulation of biological process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of response to stimulus positive regulation of metabolic process positive regulation of DNA repair cellular response to stress response to stress regulation of response to stress positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of cellular process regulation of double-strand break repair via nonhomologous end joining DNA repair double-strand break repair regulation of biological process positive regulation of nucleobase-containing compound metabolic process positive regulation of double-strand break repair via nonhomologous end joining double-strand break repair via nonhomologous end joining primary metabolic process DNA metabolic process regulation of macromolecule metabolic process nucleic acid metabolic process positive regulation of double-strand break repair regulation of DNA repair positive regulation of DNA metabolic process DNA damage response regulation of response to stimulus metabolic process cellular process macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process DNA-templated DNA replication DNA replication DNA ligation involved in DNA repair DNA ligation response to radiation response to abiotic stimulus nucleotide-excision repair phosphorylation phosphate-containing compound metabolic process phosphorus metabolic process base-excision repair, gap-filling base-excision repair positive regulation of organelle organization regulation of telomere maintenance regulation of cellular component organization positive regulation of cellular component organization telomere organization cellular component organization cellular component organization or biogenesis positive regulation of telomere maintenance chromosome organization regulation of chromosome organization regulation of organelle organization positive regulation of chromosome organization telomere maintenance organelle organization response to oxidative stress	membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle membraneless organelle nuclear lumen membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle membraneless organelle nuclear lumen membrane nucleoplasm site of double-strand break site of DNA damage chromosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR006551	Polynucleotide 3'-phosphatase	Domain
A	IPR041388	PNK, FHA domain	Domain
A	IPR006550	Bifunctional polynucleotide phosphatase/kinase PNKP	Family
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A	IPR006549	HAD-superfamily hydrolase,subfamily IIIA	Domain
A	IPR023214	HAD superfamily	Homologous Superfamily
A	IPR008984	SMAD/FHA domain superfamily	Homologous Superfamily
A	IPR013954	Polynucleotide kinase 3 phosphatase	Family
A	IPR036412	HAD-like superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: Q96T60	microcephaly, seizures, and developmental delay microcephaly ataxia - oculomotor apraxia type 4 intellectual disability visual epilepsy Epileptic Seizures ovarian cancer developmental disability neurodegenerative disease developmental and epileptic encephalopathy DNA repair disease microlissencephaly osteosarcoma psoriasis spinocerebellar ataxia, autosomal recessive, with axonal neuropathy 1 microcephaly, seizures, and developmental delay microcephaly ataxia - oculomotor apraxia type 4 intellectual disability visual epilepsy Epileptic Seizures ovarian cancer developmental disability neurodegenerative disease developmental and epileptic encephalopathy DNA repair disease microlissencephaly osteosarcoma psoriasis spinocerebellar ataxia, autosomal recessive, with axonal neuropathy 1 epilepsy tuberculosis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.