2NWN

New Pharmacophore for Serine Protease Inhibition Revealed by Crystal Structure of Human Urokinase-type Plasminogen Activator Complexed with a Cyclic Peptidyl Inhibitor, upain-1

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Trypsin-like serine proteases	8042216	3000114	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Trypsin_1	e2nwnA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: Trypsin_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00089	Trypsin (Trypsin)	Trypsin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Plasminogen activator, urokinase	peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of biological process cell adhesion regulation of cellular process multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of biological process cell adhesion regulation of cellular process regulation of cell adhesion cellular process muscle cell migration cell migration cell motility smooth muscle cell migration cell adhesion mediated by integrin regulation of cell adhesion mediated by integrin regulation of response to external stimulus negative regulation of biological process regulation of response to wounding regulation of coagulation regulation of response to stress regulation of multicellular organismal process regulation of hemostasis negative regulation of response to stimulus negative regulation of wound healing regulation of blood coagulation negative regulation of blood coagulation response to external stimulus negative regulation of response to wounding negative regulation of hemostasis fibrinolysis negative regulation of coagulation regulation of response to stimulus negative regulation of response to external stimulus regulation of wound healing negative regulation of multicellular organismal process zymogen activation primary metabolic process gene expression macromolecule biosynthetic process protein maturation biosynthetic process proteolysis protein processing metabolic process protein metabolic process macromolecule metabolic process plasminogen activation regulation of cell motility regulation of smooth muscle cell migration regulation of locomotion regulation of cell migration locomotion cellular response to stimulus signaling signal transduction cell communication regulation of protein maturation regulation of primary metabolic process regulation of proteolysis regulation of plasminogen activation regulation of biosynthetic process regulation of protein metabolic process regulation of macromolecule metabolic process regulation of gene expression regulation of protein processing regulation of macromolecule biosynthetic process regulation of metabolic process chemotaxis taxis response to chemical positive regulation of coagulation positive regulation of biological process positive regulation of blood coagulation positive regulation of response to stimulus positive regulation of response to external stimulus positive regulation of hemostasis positive regulation of response to wounding positive regulation of wound healing regulation of fibrinolysis positive regulation of multicellular organismal process negative regulation of fibrinolysis negative regulation of plasminogen activation negative regulation of proteolysis negative regulation of protein maturation negative regulation of cellular process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process negative regulation of metabolic process negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of protein processing positive regulation of locomotion positive regulation of cell migration positive regulation of cell motility positive regulation of cellular process response to decreased oxygen levels response to hypoxia response to oxygen levels response to abiotic stimulus cell population proliferation regulation of cell population proliferation regulation of signaling regulation of signaling receptor activity regulation of signal transduction regulation of molecular function regulation of cell communication regulation of cell-substrate adhesion cell-substrate adhesion regulation of smooth muscle cell-matrix adhesion smooth muscle cell-matrix adhesion cell-matrix adhesion regulation of cell-matrix adhesion cell surface receptor signaling pathway urokinase plasminogen activator signaling pathway	membrane-bounded organelle intracellular anatomical structure tertiary granule membrane membrane intracellular vesicle intracellular membrane-bounded organelle endomembrane system tertiary granule intracellular organelle cytoplasmic vesicle membrane cellular anatomical structure bounding membrane of organelle vesicle membrane cytoplasmic vesicle membrane-bounded organelle intracellular anatomical structure tertiary granule membrane membrane intracellular vesicle intracellular membrane-bounded organelle endomembrane system tertiary granule intracellular organelle cytoplasmic vesicle membrane cellular anatomical structure bounding membrane of organelle vesicle membrane cytoplasmic vesicle organelle secretory granule secretory vesicle cytoplasm vesicle organelle membrane secretory granule membrane anchoring junction cell junction focal adhesion cell-substrate junction specific granule membrane specific granule cell periphery cell surface plasma membrane external side of plasma membrane side of membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space extracellular membrane-bounded organelle protein-containing complex serine protease inhibitor complex protease inhibitor complex peptidase inhibitor complex catalytic complex peptidase complex endopeptidase complex serine-type endopeptidase complex serine-type peptidase complex protein complex involved in cell-matrix adhesion protein complex involved in cell adhesion
B	upain-1	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR018056	Kringle, conserved site	Conserved Site
A	IPR000742	EGF-like domain	Domain
A	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site
A	IPR013806	Kringle-like fold	Homologous Superfamily
A	IPR038178	Kringle superfamily	Homologous Superfamily
A	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A	IPR001314	Peptidase S1A, chymotrypsin family	Family
A	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
A	IPR001254	Serine proteases, trypsin domain	Domain
A	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
A	IPR050127	Serine Proteases (Peptidase S1 Family)	Family
A	IPR000001	Kringle	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P00749	Quebec platelet disorder Alzheimer disease idiopathic pulmonary fibrosis transitional cell carcinoma Henoch-Schoenlein purpura IgA glomerulonephritis Venous Thrombosis malignant pancreatic neoplasm adenocarcinoma cerebrovascular disorder cholesterol embolism tubular adenocarcinoma ventricular fibrillation psoriasis granular cell carcinoma Quebec platelet disorder Alzheimer disease idiopathic pulmonary fibrosis transitional cell carcinoma Henoch-Schoenlein purpura IgA glomerulonephritis Venous Thrombosis malignant pancreatic neoplasm adenocarcinoma cerebrovascular disorder cholesterol embolism tubular adenocarcinoma ventricular fibrillation psoriasis granular cell carcinoma cerebral infarction myocardial infarction intermediate coronary syndrome cirrhosis of liver anuria nephritis brain edema brain ischemia prostate cancer carotid artery disease pulmonary embolism middle cerebral artery infarction sagittal sinus thrombosis asthma ischemia reperfusion injury bone cancer pilocytic astrocytoma gastric carcinoma breast cancer malignant glioma hereditary diffuse gastric adenocarcinoma idiopathic interstitial pneumonia multiple sclerosis inflammatory bowel disease nasopharyngeal carcinoma interstitial cystitis coronary artery disease osteosarcoma cribriform carcinoma of breast invasive ductal breast carcinoma atopic eczema pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia ischemic disease primitive neuroectodermal tumor gastric neoplasm malignant colon neoplasm cancer Alzheimer disease type 1 thrombotic disease interstitial lung disease ulcerative colitis intracranial hemorrhage ovarian cancer Crohn disease atypical teratoid rhabdoid tumor pancreatic neoplasm large cell medulloblastoma gastric cancer lung adenocarcinoma glioblastoma malignant ependymoma acute interstitial pneumonia hypotensive disorder congestive heart failure tuberculosis intracerebral hemorrhage non-small cell lung carcinoma cribriform carcinoma pancreatic ductal adenocarcinoma cystic fibrosis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.