2TPS

THIAMIN PHOSPHATE SYNTHASE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2tpsa1	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Thiamin phosphate synthase	Thiamin phosphate synthase	Thiamin phosphate synthase	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
A	d2tpsa2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d2tpsb1	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Thiamin phosphate synthase	Thiamin phosphate synthase	Thiamin phosphate synthase	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d2tpsb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Thiamin phosphate synthase	8030883	4003094	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thiamin phosphate synthase	8043262	3000242	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Thiamin phosphate synthase	8043262	3000242	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	TMP-TENI	e2tpsA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TMP-TENI	ECOD (1.6)
B	TMP-TENI	e2tpsB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TMP-TENI	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
B	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF02581	Thiamine monophosphate synthase (TMP-TENI)	Thiamine monophosphate synthase	Thiamine monophosphate synthase (TMP) (EC:2.5.1.3) catalyses the substitution of the pyrophosphate of 2-methyl-4-amino-5- hydroxymethylpyrimidine pyrophosphate by 4-methyl-5- (beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate [1]. This ...	Thiamine monophosphate synthase (TMP) (EC:2.5.1.3) catalyses the substitution of the pyrophosphate of 2-methyl-4-amino-5- hydroxymethylpyrimidine pyrophosphate by 4-methyl-5- (beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate [1]. This Pfam family also includes the regulatory protein TENI (Swiss:P25053), a protein from Bacillus subtilis that regulates the production of several extracellular enzymes by reducing alkaline protease production [2]. While TenI shows high sequence similarity with thiamin phosphate synthase, the purified protein has no thiamin phosphate synthase activity. Instead, it is a thiazole tautomerase [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	PROTEIN (THIAMIN PHOSPHATE SYNTHASE)	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding thiamine-phosphate diphosphorylase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity	cellular biosynthetic process thiamine-containing compound metabolic process vitamin biosynthetic process phosphate-containing compound metabolic process thiamine diphosphate metabolic process small molecule metabolic process sulfur compound metabolic process pyrimidine-containing compound biosynthetic process water-soluble vitamin metabolic process phosphorus metabolic process pyrimidine-containing compound metabolic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular metabolic process cellular biosynthetic process thiamine-containing compound metabolic process vitamin biosynthetic process phosphate-containing compound metabolic process thiamine diphosphate metabolic process small molecule metabolic process sulfur compound metabolic process pyrimidine-containing compound biosynthetic process water-soluble vitamin metabolic process phosphorus metabolic process pyrimidine-containing compound metabolic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular metabolic process vitamin metabolic process sulfur compound biosynthetic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process metabolic process cellular process thiamine diphosphate biosynthetic process thiamine-containing compound biosynthetic process organic hydroxy compound metabolic process alcohol metabolic process thiamine metabolic process thiamine biosynthetic process primary alcohol biosynthetic process organic hydroxy compound biosynthetic process alcohol biosynthetic process primary alcohol metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR034291	Thiamine phosphate synthase	Family
A, B	IPR036206	Thiamin phosphate synthase superfamily	Homologous Superfamily
A, B	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A, B	IPR022998	Thiamine phosphate synthase/TenI	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	thiamine phosphate synthase M-CSA #364	Thiamin phosphate synthase catalyses the formation of thiamin phosphate from 4-amino-5-(hydroxymethyl)-2-methylpyrimidine pyrophosphate and 5-(hydroxyethyl-4-methylthiazole phosphate, in the penultimate step of the biosynthesis of thiamin phosphate.	Defined by 3 residues: ARG:A-51 [auth A-59]SER:A-122 [auth A-130]LYS:A-151 [auth A-159] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.5.1.3 (PDB Primary Data) Search M-CSA Motif + EC 2.5.1.3

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.