3C2E
Crystal structure at 1.9A of the apo quinolinate phosphoribosyl transferase (BNA6) from Saccharomyces cerevisiae
Domain Annotation: ECOD Classification ECOD Database Homepage
Chains | Family Name | Domain Identifier | Architecture | Possible Homology | Homology | Topology | Family | Provenance Source (Version) |
---|---|---|---|---|---|---|---|---|
A | QRPTase_N | e3c2eA1 | A: a+b complex topology | X: alpha/beta-Hammerhead/Barrel-sandwich hybrid | H: alpha/beta-Hammerhead/Barrel-sandwich hybrid | T: Nicotinate/Quinolinate PRTase N-terminal domain-like | F: QRPTase_N | ECOD (1.6) |
A | QRPTase_C | e3c2eA2 | A: a/b barrels | X: TIM beta/alpha-barrel | H: TIM barrels (From Topology) | T: TIM barrels | F: QRPTase_C | ECOD (1.6) |
Domain Annotation: CATH CATH Database Homepage
Chain | Domain | Class | Architecture | Topology | Homology | Provenance Source (Version) |
---|---|---|---|---|---|---|
A | 3.90.1170.20 | Alpha Beta | Alpha-Beta Complex | Aldehyde Oxidoreductase | domain 3 | CATH (4.3.0) |
A | 3.20.20.70 | Alpha Beta | Alpha-Beta Barrel | TIM Barrel | Aldolase class I | CATH (4.3.0) |
Protein Family Annotation Pfam Database Homepage
Chains | Accession | Name | Description | Comments | Source |
---|---|---|---|---|---|
PF02749 | Quinolinate phosphoribosyl transferase, N-terminal domain (QRPTase_N) | Quinolinate phosphoribosyl transferase, N-terminal domain | Quinolinate phosphoribosyl transferase (QPRTase) or nicotinate-nucleotide pyrophosphorylase EC:2.4.2.19 is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyses the reaction of quinolinic acid with 5-phosphoribosyl ... | Domain | |
PF01729 | Quinolinate phosphoribosyl transferase, C-terminal domain (QRPTase_C) | Quinolinate phosphoribosyl transferase, C-terminal domain | Quinolinate phosphoribosyl transferase (QPRTase) or nicotinate-nucleotide pyrophosphorylase EC:2.4.2.19 is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyses the reaction of quinolinic acid with 5-phosphoribosyl ... | Domain |
Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage
InterPro: Protein Family Classification InterPro Database Homepage
Chains | Accession | Name | Type |
---|---|---|---|
IPR022412 | Quinolinate phosphoribosyl transferase, N-terminal | Domain | |
IPR037128 | Quinolinate phosphoribosyl transferase, N-terminal domain superfamily | Homologous Superfamily | |
IPR013785 | Aldolase-type TIM barrel | Homologous Superfamily | |
IPR004393 | Nicotinate-nucleotide pyrophosphorylase | Family | |
IPR002638 | Quinolinate phosphoribosyl transferase, C-terminal | Domain | |
IPR027277 | Nicotinate-nucleotide pyrophosphorylase/Putative pyrophosphorylase ModD | Family | |
IPR036068 | Nicotinate phosphoribosyltransferase-like, C-terminal | Homologous Superfamily |
Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage
Chains | Enzyme Name | Description | Catalytic Residues |
---|---|---|---|
nicotinate-nucleotide pyrophosphorylase (carboxylating) (type II) M-CSA #945 | This is a type II Quinolinic acid phosphoribosyltransferase (QAPRTase). This protein is required for the de novo biosynthesis of NAD in both prokaryotes and eukaryotes. The enzyme catalyses the reaction between quinolinic acid (QA) and 5-phosphoribosyl-1-pyrophosphate (PRPP), to yield nicotinic acid mononucleotide (NAMN), pyrophosphate and CO2, the latter resulting from decarboxylation at position 2 of the quinolinate ring. QAPRTase has been grouped with nine other enzymes, (phosphoribosyltransferases, PRTases) that catalyse chemically similar phosphoribosyl transfer reactions using the substrate PRPP. The PRTases are involved in de novo and salvage reactions of nucleotide synthesis, as well as in histidine and tryptophan biosynthesis. Type II enzymes lack the type I PRPP-binding motif and have TIM barrel-like structure [PMID:9016724]. | EC: 2.4.2.19 (PDB Primary Data) |