3FFK

Crystal structure of human Gelsolin domains G1-G3 bound to Actin

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3ffka1	Alpha and beta proteins (a+b)	Gelsolin-like	Actin depolymerizing proteins	Gelsolin-like	Gelsolin	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C [auth D]	d3ffkd1	Alpha and beta proteins (a+b)	Gelsolin-like	Actin depolymerizing proteins	Gelsolin-like	Gelsolin	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d3ffkb2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
B	d3ffkb1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
D [auth E]	d3ffke2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
D [auth E]	d3ffke1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin depolymerizing proteins	8040110	3000430	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	Actin depolymerizing proteins	8040110	3000430	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Actin-like ATPases	8040509	3000092	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Actin-like ATPases	8040506	3000092	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	Actin-like ATPases	8040506	3000092	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	Actin-like ATPases	8040509	3000092	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Gelsolin	e3ffkA1	A: a+b three layers	X: Gelsolin-like (From Topology)	H: Gelsolin-like (From Topology)	T: Gelsolin-like	F: Gelsolin	ECOD (1.6)
A	Gelsolin_1	e3ffkA2	A: a+b three layers	X: Gelsolin-like (From Topology)	H: Gelsolin-like (From Topology)	T: Gelsolin-like	F: Gelsolin_1	ECOD (1.6)
C [auth D]	Gelsolin	e3ffkD1	A: a+b three layers	X: Gelsolin-like (From Topology)	H: Gelsolin-like (From Topology)	T: Gelsolin-like	F: Gelsolin	ECOD (1.6)
C [auth D]	Gelsolin_1	e3ffkD2	A: a+b three layers	X: Gelsolin-like (From Topology)	H: Gelsolin-like (From Topology)	T: Gelsolin-like	F: Gelsolin_1	ECOD (1.6)
B	Actin_1st	e3ffkB3	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
B	Actin_2nd	e3ffkB4	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)
D [auth E]	Actin_1st	e3ffkE3	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
D [auth E]	Actin_2nd	e3ffkE4	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.20.10	Alpha Beta	3-Layer(aba) Sandwich	Severin	Severin	CATH (4.3.0)
C [auth D]	3.40.20.10	Alpha Beta	3-Layer(aba) Sandwich	Severin	Severin	CATH (4.3.0)
B	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
B	2.30.36.70	Mainly Beta	Roll	Actin	Chain A, domain 2	CATH (4.3.0)
B	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
D [auth E]	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
D [auth E]	2.30.36.70	Mainly Beta	Roll	Actin	Chain A, domain 2	CATH (4.3.0)
D [auth E]	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth D]	PF00626	Gelsolin repeat (Gelsolin)	Gelsolin repeat	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth D]	plasma gelsolin	cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding myosin II binding myosin binding cation binding ion binding calcium ion binding metal ion binding small molecule binding phosphatidylinositol 3-kinase catalytic subunit binding cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding myosin II binding myosin binding cation binding ion binding calcium ion binding metal ion binding small molecule binding phosphatidylinositol 3-kinase catalytic subunit binding phosphatidylinositol 3-kinase binding kinase binding enzyme binding phosphatidylinositol-4,5-bisphosphate binding anion binding phosphatidylinositol bisphosphate binding phospholipid binding lipid binding phosphatidylinositol phosphate binding phosphatidylinositol binding	membrane organization import into cell transport establishment of localization vesicle-mediated transport cellular component organization localization phagocytosis, engulfment membrane invagination cellular process phagocytosis cellular component organization or biogenesis endocytosis plasma membrane invagination membrane organization import into cell transport establishment of localization vesicle-mediated transport cellular component organization localization phagocytosis, engulfment membrane invagination cellular process phagocytosis cellular component organization or biogenesis endocytosis plasma membrane invagination positive regulation of epithelial cell apoptotic process regulation of biological process regulation of cellular process positive regulation of programmed cell death regulation of epithelial cell apoptotic process positive regulation of keratinocyte apoptotic process regulation of apoptotic process biological regulation positive regulation of biological process regulation of programmed cell death cell death positive regulation of apoptotic process epithelial cell apoptotic process keratinocyte apoptotic process regulation of keratinocyte apoptotic process apoptotic process programmed cell death positive regulation of cellular process developmental process multicellular organism development multicellular organismal process central nervous system development anatomical structure development nervous system development system development protein destabilization regulation of protein stability regulation of biological quality regulation of biosynthetic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process positive regulation of macromolecule biosynthetic process regulation of gene expression positive regulation of metabolic process biosynthetic process positive regulation of gene expression positive regulation of biosynthetic process regulation of macromolecule biosynthetic process metabolic process positive regulation of macromolecule metabolic process macromolecule metabolic process regulation of metabolic process actin filament severing actin filament-based process regulation of localization cellular localization plasma membrane raft distribution regulation of cellular component organization plasma membrane raft polarization plasma membrane organization membrane raft organization endomembrane system organization membrane raft distribution plasma membrane raft localization membrane raft polarization regulation of plasma membrane raft polarization localization within membrane membrane raft localization regulation of plasma membrane organization regulation of cellular localization cellular component disassembly actin polymerization or depolymerization actin cytoskeleton organization protein-containing complex organization actin filament organization protein-containing complex disassembly actin filament depolymerization organelle organization supramolecular fiber organization protein depolymerization cytoskeleton organization protein localization regulation of protein localization protein localization to membrane cellular macromolecule localization macromolecule localization receptor clustering regulation of receptor clustering regulation of protein localization to membrane regulation of protein maturation positive regulation of protein maturation positive regulation of protein metabolic process regulation of protein processing in phagocytic vesicle positive regulation of protein processing regulation of primary metabolic process protein maturation regulation of proteolysis proteolysis positive regulation of protein processing in phagocytic vesicle protein metabolic process regulation of protein metabolic process primary metabolic process protein processing in phagocytic vesicle regulation of protein processing positive regulation of proteolysis protein processing regulation of cellular component size negative regulation of biological process regulation of protein-containing complex disassembly regulation of actin filament depolymerization cellular component biogenesis actin filament capping cellular component assembly negative regulation of supramolecular fiber organization negative regulation of actin filament polymerization barbed-end actin filament capping negative regulation of cellular component organization regulation of organelle organization negative regulation of protein depolymerization negative regulation of protein-containing complex assembly regulation of protein polymerization regulation of supramolecular fiber organization regulation of cytoskeleton organization regulation of actin filament-based process regulation of protein-containing complex assembly negative regulation of protein-containing complex disassembly negative regulation of cellular process protein-containing complex assembly negative regulation of protein polymerization regulation of actin filament polymerization protein polymerization regulation of actin filament organization actin filament polymerization regulation of protein depolymerization negative regulation of cytoskeleton organization regulation of actin polymerization or depolymerization regulation of cellular component biogenesis negative regulation of organelle organization regulation of actin filament length regulation of actin cytoskeleton organization negative regulation of actin filament depolymerization regulation of anatomical structure size renal system process system process renal absorption renal protein absorption regulation of establishment of T cell polarity regulation of establishment or maintenance of cell polarity establishment of lymphocyte polarity establishment of T cell polarity cell activation establishment or maintenance of cell polarity lymphocyte activation leukocyte activation establishment of cell polarity regulation of establishment of cell polarity immune system process T cell activation relaxation of cardiac muscle muscle system process relaxation of muscle positive regulation of organelle organization positive regulation of cellular component organization regulation of actin nucleation positive regulation of cytoskeleton organization positive regulation of supramolecular fiber organization positive regulation of actin nucleation actin nucleation amyloid fibril formation muscle atrophy striated muscle adaptation muscle adaptation response to stimulus striated muscle atrophy biological process involved in interspecies interaction between organisms negative regulation of viral life cycle viral life cycle modulation by symbiont of entry into host biological process involved in interaction with host biological process involved in symbiotic interaction regulation of viral entry into host cell regulation of viral life cycle regulation of viral process negative regulation of viral process symbiont entry into host cell viral process symbiont entry into host negative regulation of viral entry into host cell regulation of biological process involved in symbiotic interaction organelle assembly plasma membrane bounded cell projection organization cell projection organization plasma membrane bounded cell projection assembly cilium assembly cilium organization cell projection assembly immune response response to other organism defense response response to type II interferon response to cytokine defense response to symbiont response to external stimulus cellular response to type II interferon response to biotic stimulus response to peptide response to chemical defense response to other organism response to stress innate immune response cellular response to cytokine stimulus response to external biotic stimulus hepatocyte apoptotic process podosome assembly membraneless organelle assembly regulation of podosome assembly regulation of organelle assembly maintenance of location maintenance of protein location maintenance of location in cell maintenance of protein location in cell sequestering of actin monomers heart process cardiac muscle cell contraction actin filament-based movement cardiac muscle contraction muscle contraction striated muscle contraction circulatory system process actin-mediated cell contraction heart contraction blood circulation response to mechanical stimulus response to abiotic stimulus response to muscle stretch	cell projection cellular anatomical structure cell leading edge plasma membrane bounded cell projection lamellipodium intracellular organelle intracellular anatomical structure podosome cytoskeleton organelle membraneless organelle intracellular membraneless organelle actin cytoskeleton extracellular exosome cell projection cellular anatomical structure cell leading edge plasma membrane bounded cell projection lamellipodium intracellular organelle intracellular anatomical structure podosome cytoskeleton organelle membraneless organelle intracellular membraneless organelle actin cytoskeleton extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cytoplasm cytosol sarcoplasm anchoring junction cell junction focal adhesion cell-substrate junction intracellular vesicle phagocytic vesicle intracellular membrane-bounded organelle cytoplasmic vesicle endocytic vesicle cell periphery plasma membrane membrane cell cortex cortical cytoskeleton actin cap cortical actin cytoskeleton membrane-enclosed lumen ficolin-1-rich granule endomembrane system intracellular organelle lumen organelle lumen ficolin-1-rich granule lumen secretory granule secretory vesicle blood microparticle vesicle lumen cytoplasmic vesicle lumen secretory granule lumen
B, D [auth E]	actin, alpha skeletal muscle	cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal motor regulator activity molecular function activator activity molecular function regulator activity magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding myosin heavy chain binding myosin binding tropomyosin binding calcium-dependent protein binding identical protein binding actin monomer binding actin binding	developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development cellular developmental process skeletal muscle tissue development cell development muscle cell differentiation myotube differentiation striated muscle tissue development muscle structure development skeletal muscle organ development striated muscle cell development muscle cell development cell differentiation cellular process muscle tissue development muscle organ development striated muscle cell differentiation regulation of biosynthetic process regulation of biological process regulation of cellular process biological regulation positive regulation of biological process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process positive regulation of macromolecule biosynthetic process regulation of gene expression positive regulation of metabolic process biosynthetic process positive regulation of gene expression positive regulation of biosynthetic process regulation of macromolecule biosynthetic process metabolic process positive regulation of macromolecule metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular process cellular component assembly involved in morphogenesis skeletal myofibril assembly membraneless organelle assembly cellular component organization cellular component biogenesis cellular anatomical entity morphogenesis cellular component assembly actin filament-based process cellular component organization or biogenesis skeletal muscle thin filament assembly actin cytoskeleton organization actomyosin structure organization organelle assembly actin filament organization organelle organization myofibril assembly anatomical structure formation involved in morphogenesis supramolecular fiber organization cytoskeleton organization protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization protein-containing complex assembly actin filament bundle organization actin filament bundle assembly	intracellular anatomical structure skeletal muscle myofibril intracellular membraneless organelle intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex cell projection intracellular anatomical structure skeletal muscle myofibril intracellular membraneless organelle intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex cell projection cell leading edge plasma membrane bounded cell projection lamellipodium cytoskeleton actin filament bundle actin cytoskeleton actomyosin stress fiber contractile actin filament bundle polymeric cytoskeletal fiber actin filament protein-containing complex filamentous actin cell body striated muscle thin filament myofilament sarcomere actin-based cell projection filopodium

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth D]	IPR007123	Gelsolin-like domain	Domain
A, C [auth D]	IPR029006	ADF-H/Gelsolin-like domain superfamily	Homologous Superfamily
A, C [auth D]	IPR007122	Villin/Gelsolin	Family
B, D [auth E]	IPR020902	Actin/actin-like conserved site	Conserved Site
B, D [auth E]	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
B, D [auth E]	IPR004001	Actin, conserved site	Conserved Site
B, D [auth E]	IPR004000	Actin family	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, C [auth D]	Pharos: P06396	Finnish type amyloidosis amyloidosis paratuberculosis Liver Cirrhosis, Experimental acute coronary syndrome osteoporosis ductal breast carcinoma in situ esophageal adenocarcinoma Gaucher disease tuberculosis interstitial cystitis malignant colon neoplasm adrenoleukodystrophy atrial fibrillation breast carcinoma Finnish type amyloidosis amyloidosis paratuberculosis Liver Cirrhosis, Experimental acute coronary syndrome osteoporosis ductal breast carcinoma in situ esophageal adenocarcinoma Gaucher disease tuberculosis interstitial cystitis malignant colon neoplasm adrenoleukodystrophy atrial fibrillation breast carcinoma chronic obstructive pulmonary disease ovarian cancer drug-induced liver injury pancreatic intraductal papillary-mucinous neoplasm Emery-Dreifuss muscular dystrophy periodic fever syndrome psoriasis ulcerative colitis invasive ductal breast carcinoma acquired metabolic disease malignant ependymoma malignant pancreatic neoplasm benign mesothelioma breast fibroadenoma mesothelioma pancreatic ductal adenocarcinoma Duchenne muscular dystrophy pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia endometriosis pilocytic astrocytoma schizophrenia lattice corneal dystrophy lung cancer malignant mesothelioma astrocytic tumor medulloblastoma breast cancer osteosarcoma pancreatic intraductal papillary-mucinous carcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.