3IIX

X-ray structure of the FeFe-hydrogenase maturase HydE from T. maritima in complex with methionine and 5'deoxyadenosine

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Radical_SAM	e3iixA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Radical_SAM	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF04055	Radical SAM superfamily (Radical_SAM)	Radical SAM superfamily	Radical SAM proteins catalyse diverse reactions, including unusual methylations, isomerisation, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	[FeFe] hydrogenase maturase subunit HydE	oxidoreductase activity catalytic activity iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding 4 iron, 4 sulfur cluster binding cation binding ion binding metal ion binding transferase activity	water-soluble vitamin biosynthetic process biosynthetic process small molecule biosynthetic process vitamin biosynthetic process small molecule metabolic process metabolic process cellular process water-soluble vitamin metabolic process vitamin metabolic process sulfur compound biosynthetic process sulfur compound metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR007197	Radical SAM	Domain
A	IPR034422	HydE/PylB-like	Family
A	IPR006638	Elp3/MiaA/NifB-like, radical SAM core domain	Domain
A	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A	IPR010722	Biotin and thiamin synthesis-associated domain	Domain
A	IPR024021	[FeFe]-hydrogenase maturation HydE, radical SAM	Family

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	CSO	Parent Component: CYS RESID: AA0205 PSI-MOD : L-cysteine sulfenic acid MOD:00210
A	CSS	Parent Component: CYS RESID: AA0205 , AA0269 PSI-MOD : L-cysteine sulfenic acid MOD:00210 , L-cysteine persulfide MOD:00274
A	OTY	RESID: AA0205 , AA0269

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.