4AUN

Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
H	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
D	SCOP2 Family	Heme-dependent catalases	8104646	4002736	SCOP2 (2022-06-29)
D	SCOP2 Family	Catalase, C-terminal domain	8105280	4003754	SCOP2 (2022-06-29)
D	SCOP2 Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2 (2022-06-29)
D	SCOP2 Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2 (2022-06-29)
E	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Heme-dependent catalase-like	8104647	3001681	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Class I glutamine amidotransferase-like	8105281	3001405	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
H	Catalase	e4aunH3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
H	Catalase-rel	e4aunH7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
H	GATase1_catalase	e4aunH9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
A	Catalase	e4aunA3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
A	Catalase-rel	e4aunA7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
A	GATase1_catalase	e4aunA9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
B	Catalase	e4aunB3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
B	Catalase-rel	e4aunB7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
B	GATase1_catalase	e4aunB9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
C	Catalase	e4aunC3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
C	Catalase-rel	e4aunC7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
C	GATase1_catalase	e4aunC9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
D	Catalase	e4aunD3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
D	Catalase-rel	e4aunD7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
D	GATase1_catalase	e4aunD9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
E	Catalase	e4aunE3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
E	Catalase-rel	e4aunE7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
E	GATase1_catalase	e4aunE9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
F	Catalase	e4aunF3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
F	Catalase-rel	e4aunF7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
F	GATase1_catalase	e4aunF9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)
G	Catalase	e4aunG3	A: beta barrels	X: Barrel domain in heme-dependent catalases (From Topology)	H: Barrel domain in heme-dependent catalases (From Topology)	T: Barrel domain in heme-dependent catalases	F: Catalase	ECOD (1.6)
G	Catalase-rel	e4aunG7	A: alpha bundles	X: Second alpha-helical domain in heme-dependent catalases (From Topology)	H: Second alpha-helical domain in heme-dependent catalases (From Topology)	T: Second alpha-helical domain in heme-dependent catalases	F: Catalase-rel	ECOD (1.6)
G	GATase1_catalase	e4aunG9	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Class I glutamine amidotransferase-like	F: GATase1_catalase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
H	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
H	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
H	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
A	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
A	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
A	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
B	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
B	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
B	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
C	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
C	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
C	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
D	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
D	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
D	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
E	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
E	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
E	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
F	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
F	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
F	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)
G	2.40.180.10	Mainly Beta	Beta Barrel	Catalase HpII, Chain A, domain 1	Catalase core domain	CATH (4.3.0)
G	1.20.1370.20	Mainly Alpha	Up-down Bundle	Hemocyanin, N-terminal domain	Catalase, four-helical domain	CATH (4.3.0)
G	3.40.50.880	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Class I glutamine amidotransferase (GATase) domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF18011	C-terminal domain found in long catalases (Catalase_C)	C-terminal domain found in long catalases	This domain is found at the C-terminus of a variety of large catalase enzymes from bacteria. Structurally it is related to class I glutamine amidotransferase domains. The precise molecular function of this domain is uncertain.	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	PF06628	Catalase-related immune-responsive (Catalase-rel)	Catalase-related immune-responsive	-	Family
A, B, C, D, E A, B, C, D, E, F, G, H	PF00199	Catalase (Catalase)	Catalase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	CATALASE-PHENOL OXIDASE	peroxidase activity oxidoreductase activity antioxidant activity catalase activity oxidoreductase activity, acting on peroxide as acceptor catalytic activity binding organic cyclic compound binding tetrapyrrole binding heme binding cation binding ion binding metal ion binding small molecule binding	cellular catabolic process catabolic process cellular metabolic process hydrogen peroxide metabolic process reactive oxygen species metabolic process metabolic process cellular process hydrogen peroxide catabolic process response to oxidative stress response to stimulus response to stress	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR029062	Class I glutamine amidotransferase-like	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR024708	Catalase active site	Active Site
A, B, C, D, E A, B, C, D, E, F, G, H	IPR041399	Large catalase, C-terminal domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR010582	Catalase immune-responsive domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR043156	Catalase, mono-functional, haem-containing, clade 2, helical domain	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR002226	Catalase haem-binding site	Binding Site
A, B, C, D, E A, B, C, D, E, F, G, H	IPR020835	Catalase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR024712	Catalase, mono-functional, haem-containing, clade 2	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR011614	Catalase core domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR018028	Catalase, mono-functional, haem-containing	Family