Annotations: 4CCE

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glyco_hydro_59_C	e4cceA1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Concanavalin A-like lectins/glucanases	F: Glyco_hydro_59_C	ECOD (1.6)
A	Glyco_hydro_59M	e4cceA2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycosyl hydrolase domain (From Topology)	T: Glycosyl hydrolase domain	F: Glyco_hydro_59M	ECOD (1.6)
A	Glyco_hydro_59_N	e4cceA3	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_59_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
A	2.60.120.560	Mainly Beta	Sandwich	Jelly Rolls	Exo-inulinase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF17387	Glycosyl hydrolase family 59 central domain (Glyco_hydro_59M)	Glycosyl hydrolase family 59 central domain	-	Domain
A	PF21708	Galactocerebrosidase, C-terminal lectin domain (Glyco_hydro_59_C)	Galactocerebrosidase, C-terminal lectin domain	This domain is found at the C-terminal end of Galactocerebrosidase from Mus musculus (GALCERase) and similar proteins predominantly found in animals and bacteria. GALCERase hydrolyses the galactose ester bonds of glycolipids such as galactosylceramid ...	This domain is found at the C-terminal end of Galactocerebrosidase from Mus musculus (GALCERase) and similar proteins predominantly found in animals and bacteria. GALCERase hydrolyses the galactose ester bonds of glycolipids such as galactosylceramide and galactosylsphingosine. It is responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. This protein is organised into three domains, all of them contributing residues to the substrate- binding pocket. This entry represents the lectin domain, which is not present in other lysosomal enzymes, is likely to be be involved in processes that are not found in related enzymes. Mutations in this domain lead to Krabbe disease [1].	Domain
A	PF02057	Glycosyl hydrolase family 59 (Glyco_hydro_59)	Glycosyl hydrolase family 59	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	GALACTOCEREBROSIDASE	hydrolase activity, acting on glycosyl bonds glycosylceramidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity galactosylceramidase activity catalytic activity	myelination developmental process multicellular organism development multicellular organismal process axon ensheathment ensheathment of neurons anatomical structure development cellular process nervous system development system development sphingolipid catabolic process catabolic process glycolipid metabolic process carbohydrate derivative metabolic process myelination developmental process multicellular organism development multicellular organismal process axon ensheathment ensheathment of neurons anatomical structure development cellular process nervous system development system development sphingolipid catabolic process catabolic process glycolipid metabolic process carbohydrate derivative metabolic process galactolipid catabolic process ceramide catabolic process amide metabolic process glycosylceramide metabolic process galactolipid metabolic process membrane lipid metabolic process ceramide metabolic process carbohydrate derivative catabolic process lipid catabolic process glycosphingolipid catabolic process glycosphingolipid metabolic process galactosylceramide catabolic process liposaccharide metabolic process primary metabolic process sphingolipid metabolic process membrane lipid catabolic process glycosylceramide catabolic process glycolipid catabolic process metabolic process galactosylceramide metabolic process lipid metabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle lytic vacuole vacuole lysosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR049161	Glycosyl hydrolase family 59, catalytic domain	Domain
A	IPR001286	Glycoside hydrolase, family 59	Family
A	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A	IPR035394	Glycosyl hydrolase family 59, central domain	Domain
A	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A	IPR049162	Glycosyl hydrolase family 59, C-terminal lectin domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.