4FZW

Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4fzwa_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
B	d4fzwb1	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
B	d4fzwb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
C	d4fzwc_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
D	d4fzwd_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Crotonase-like	8067176	4000036	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	ClpP/crotonase	8067177	3000033	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	ClpP/crotonase	8067177	3000033	SCOP2B (2022-06-29)
C	SCOP2 Family	Crotonase-like	8059907	4000036	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	ClpP/crotonase	8059908	3000033	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	ClpP/crotonase	8059908	3000033	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ECH_1	e4fzwA1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: ECH_1	ECOD (1.6)
B	ECH_1	e4fzwB1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: ECH_1	ECOD (1.6)
C	ECH_1	e4fzwC1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: ECH_1	ECOD (1.6)
D	ECH_1	e4fzwD1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: ECH_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
A	1.10.12.10	Mainly Alpha	Orthogonal Bundle	Lyase 2-enoyl-coa Hydratase	Chain A, domain 2	CATH (4.3.0)
B	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
B	1.10.12.10	Mainly Alpha	Orthogonal Bundle	Lyase 2-enoyl-coa Hydratase	Chain A, domain 2	CATH (4.3.0)
C	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
C	1.10.12.10	Mainly Alpha	Orthogonal Bundle	Lyase 2-enoyl-coa Hydratase	Chain A, domain 2	CATH (4.3.0)
D	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
D	1.10.12.10	Mainly Alpha	Orthogonal Bundle	Lyase 2-enoyl-coa Hydratase	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain
C, D	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	2,3-dehydroadipyl-CoA hydratase	enoyl-CoA hydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity identical protein binding protein binding binding	cellular response to stimulus catabolic process response to stimulus oxoacid metabolic process organic acid catabolic process organic acid metabolic process response to xenobiotic stimulus cellular catabolic process monocarboxylic acid catabolic process carboxylic acid catabolic process small molecule metabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process cellular response to stimulus catabolic process response to stimulus oxoacid metabolic process organic acid catabolic process organic acid metabolic process response to xenobiotic stimulus cellular catabolic process monocarboxylic acid catabolic process carboxylic acid catabolic process small molecule metabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process carboxylic acid metabolic process xenobiotic catabolic process xenobiotic metabolic process cellular response to chemical stimulus response to chemical phenylacetate catabolic process metabolic process cellular process monocarboxylic acid metabolic process small molecule catabolic process cellular lipid catabolic process fatty acid oxidation fatty acid beta-oxidation lipid oxidation fatty acid metabolic process lipid catabolic process primary metabolic process lipid modification fatty acid catabolic process cellular lipid metabolic process lipid metabolic process	protein-containing complex catalytic complex
C, D	1,2-epoxyphenylacetyl-CoA isomerase	isomerase activity catalytic activity lyase activity identical protein binding protein binding binding	cellular response to stimulus catabolic process response to stimulus oxoacid metabolic process organic acid catabolic process organic acid metabolic process response to xenobiotic stimulus cellular catabolic process monocarboxylic acid catabolic process carboxylic acid catabolic process small molecule metabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process cellular response to stimulus catabolic process response to stimulus oxoacid metabolic process organic acid catabolic process organic acid metabolic process response to xenobiotic stimulus cellular catabolic process monocarboxylic acid catabolic process carboxylic acid catabolic process small molecule metabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process carboxylic acid metabolic process xenobiotic catabolic process xenobiotic metabolic process cellular response to chemical stimulus response to chemical phenylacetate catabolic process metabolic process cellular process monocarboxylic acid metabolic process small molecule catabolic process cellular lipid catabolic process fatty acid oxidation fatty acid beta-oxidation lipid oxidation fatty acid metabolic process lipid catabolic process primary metabolic process lipid modification fatty acid catabolic process cellular lipid metabolic process lipid metabolic process	protein-containing complex catalytic complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001753	Enoyl-CoA hydratase/isomerase	Family
A, B	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
A, B	IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	Conserved Site
A, B	IPR014748	Enoyl-CoA hydratase, C-terminal	Homologous Superfamily
C, D	IPR001753	Enoyl-CoA hydratase/isomerase	Family
C, D	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
C, D	IPR011968	Phenylacetate degradation probable enoyl-CoA hydratase PaaB	Family
C, D	IPR014748	Enoyl-CoA hydratase, C-terminal	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.