4PIP

Engineered EgtD variant EgtD-M252V,E282A in complex with tryptophan and SAH

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8090824	3000118	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8090824	3000118	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8090824	3000118	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8090824	3000118	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Methyltransf_33	e4pipA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_33	ECOD (1.6)
B	Methyltransf_33	e4pipB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_33	ECOD (1.6)
C	Methyltransf_33	e4pipC1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_33	ECOD (1.6)
D	Methyltransf_33	e4pipD1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_33	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
C	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
D	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF10017	Histidine-specific methyltransferase, SAM-dependent (Methyltransf_33)	Histidine-specific methyltransferase, SAM-dependent	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Histidine-specific methyltransferase EgtD	catalytic activity, acting on a protein transferase activity, transferring one-carbon groups protein methyltransferase activity transferase activity methyltransferase activity catalytic activity	methylation metabolic process ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide amino-acid betaine biosynthetic process oxoacid metabolic process sulfur amino acid metabolic process cellular biosynthetic process organic acid metabolic process organic acid biosynthetic process ergothioneine metabolic process carboxylic acid biosynthetic process sulfur compound metabolic process small molecule metabolic process small molecule biosynthetic process methylation metabolic process ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide amino-acid betaine biosynthetic process oxoacid metabolic process sulfur amino acid metabolic process cellular biosynthetic process organic acid metabolic process organic acid biosynthetic process ergothioneine metabolic process carboxylic acid biosynthetic process sulfur compound metabolic process small molecule metabolic process small molecule biosynthetic process cellular modified histidine metabolic process cellular metabolic process carboxylic acid metabolic process amino-acid betaine metabolic process cellular modified amino acid metabolic process sulfur compound biosynthetic process biosynthetic process cellular modified amino acid biosynthetic process ergothioneine biosynthetic process cellular process cellular modified histidine biosynthetic process sulfur amino acid biosynthetic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR017804	Methyltransferase EgtD-like	Family
A, B, C, D	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B, C, D	IPR051128	EgtD Methyltransferase Superfamily	Family
A, B, C, D	IPR019257	Histidine-specific methyltransferase, SAM-dependent	Domain
A, B, C, D	IPR035094	Histidine N-alpha-methyltransferase	Family
A, B, C, D	IPR032888	Histidine N-alpha-methyltransferase, Actinobacteria-type	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.