4QVH

Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Type 2 solute binding protein-like	8058187	3000083	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Type 2 solute binding protein-like	8058187	3000083	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ACPS	e4qvhA2	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: ACPS	ECOD (1.6)
A	EntD	e4qvhA1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: EntD	ECOD (1.6)
A	SBP_bac_1_C,SBP_bac_1_N_2	e4qvhA3	A: a/b three-layered sandwiches	X: Periplasmic binding protein-like II (From Topology)	H: Periplasmic binding protein-like II (From Topology)	T: Periplasmic binding protein-like II	F: SBP_bac_1_C,SBP_bac_1_N_2	ECOD (1.6)
B	ACPS	e4qvhB2	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: ACPS	ECOD (1.6)
B	EntD	e4qvhB3	A: a+b three layers	X: Bacillus chorismate mutase-like	H: 4'-phosphopantetheinyl transferase (From Topology)	T: 4'-phosphopantetheinyl transferase	F: EntD	ECOD (1.6)
B	SBP_bac_1_C,SBP_bac_1_N_2	e4qvhB4	A: a/b three-layered sandwiches	X: Periplasmic binding protein-like II (From Topology)	H: Periplasmic binding protein-like II (From Topology)	T: Periplasmic binding protein-like II	F: SBP_bac_1_C,SBP_bac_1_N_2	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF01547	Bacterial extracellular solute-binding protein (SBP_bac_1)	Bacterial extracellular solute-binding protein	-	Family
A, B	PF17837	4'-phosphopantetheinyl transferase N-terminal domain (4PPT_N)	4'-phosphopantetheinyl transferase N-terminal domain	This entry represents the N-terminal domain from 4'- phosphopantetheinyl transferase enzymes. This domain is structurally related to the Pfam:PF01648 domain with which it forms a pseudodimeric arrangement [2].	Domain
A, B	PF01648	4'-phosphopantetheinyl transferase superfamily (ACPS)	4'-phosphopantetheinyl transferase superfamily	Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of Pfam:PF00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of ...	Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of Pfam:PF00550. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP [1]. This superfamily consists of two subtypes: The ACPS type such as Swiss:P24224 and the Sfp type such as Swiss:P39135. The structure of the Sfp type is known [3], which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Maltose-binding periplasmic protein, 4'-phosphopantetheinyl transferase chimera	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding holo-[acyl-carrier-protein] synthase activity transferase activity catalytic activity phosphotransferase activity, for other substituted phosphate groups transferase activity, transferring phosphorus-containing groups transporter activity carbohydrate transmembrane transporter activity transmembrane transporter activity cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding holo-[acyl-carrier-protein] synthase activity transferase activity catalytic activity phosphotransferase activity, for other substituted phosphate groups transferase activity, transferring phosphorus-containing groups transporter activity carbohydrate transmembrane transporter activity transmembrane transporter activity oligosaccharide binding disaccharide binding carbohydrate binding maltose binding	enterobactin biosynthetic process polyketide metabolic process organic hydroxy compound metabolic process catechol-containing compound metabolic process secondary metabolic process antibiotic biosynthetic process siderophore biosynthetic process antibiotic metabolic process secondary metabolite biosynthetic process siderophore metabolic process lactone biosynthetic process catechol-containing compound biosynthetic process catechol-containing siderophore biosynthetic process enterobactin metabolic process enterobactin biosynthetic process polyketide metabolic process organic hydroxy compound metabolic process catechol-containing compound metabolic process secondary metabolic process antibiotic biosynthetic process siderophore biosynthetic process antibiotic metabolic process secondary metabolite biosynthetic process siderophore metabolic process lactone biosynthetic process catechol-containing compound biosynthetic process catechol-containing siderophore biosynthetic process enterobactin metabolic process macrolide biosynthetic process macrolide metabolic process biosynthetic process peptide biosynthetic process polyketide biosynthetic process lactone metabolic process organic hydroxy compound biosynthetic process phenol-containing compound metabolic process phenol-containing compound biosynthetic process metabolic process cellular process nonribosomal peptide biosynthetic process peptide metabolic process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cell chemotaxis chemotaxis response to external stimulus cellular response to chemical stimulus taxis response to chemical cell migration cell motility locomotion localization transport carbohydrate transport oligosaccharide transport disaccharide transport establishment of localization maltose transport polysaccharide localization dextrin transport macromolecule localization maltodextrin transmembrane transport polysaccharide transport response to disaccharide response to oxygen-containing compound detection of maltose stimulus detection of stimulus response to carbohydrate detection of chemical stimulus detection of disaccharide stimulus response to maltose detection of carbohydrate stimulus	cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex catalytic complex enterobactin synthetase complex intracellular protein-containing complex ATPase dependent transmembrane transport complex plasma membrane protein complex membrane protein complex transporter complex ATP-binding cassette (ABC) transporter complex transmembrane transporter complex cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex catalytic complex enterobactin synthetase complex intracellular protein-containing complex ATPase dependent transmembrane transport complex plasma membrane protein complex membrane protein complex transporter complex ATP-binding cassette (ABC) transporter complex transmembrane transporter complex ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing maltose transport complex periplasmic space outer membrane-bounded periplasmic space cell envelope

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR037143	4'-phosphopantetheinyl transferase domain superfamily	Homologous Superfamily
A, B	IPR003542	Enterobactin synthetase-like, component D	Family
A, B	IPR041354	4'-phosphopantetheinyl transferase, N-terminal domain	Domain
A, B	IPR008278	4'-phosphopantetheinyl transferase domain	Domain
A, B	IPR006061	Solute-binding family 1, conserved site	Conserved Site
A, B	IPR006060	Maltose/Cyclodextrin ABC transporter, substrate-binding protein	Family
A, B	IPR006059	Bacterial-type extracellular solute-binding protein	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.