4KTR

Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with isofagomine and glycerol

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Glyco_hydro_65N	e4ktrB1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
B	Glyco_hydro_65C	e4ktrB3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
B	UNK_F_TYPE	e4ktrB2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
E	Glyco_hydro_65N	e4ktrE1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
E	Glyco_hydro_65C	e4ktrE3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
E	UNK_F_TYPE	e4ktrE2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
A	Glyco_hydro_65N	e4ktrA1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
A	Glyco_hydro_65C	e4ktrA3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
A	UNK_F_TYPE	e4ktrA2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
C	Glyco_hydro_65N	e4ktrC1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
C	Glyco_hydro_65C	e4ktrC3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
C	UNK_F_TYPE	e4ktrC2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
D	Glyco_hydro_65N	e4ktrD1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
D	Glyco_hydro_65C	e4ktrD3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
D	UNK_F_TYPE	e4ktrD2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
F	Glyco_hydro_65N	e4ktrF1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
F	Glyco_hydro_65C	e4ktrF3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
F	UNK_F_TYPE	e4ktrF2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
G	Glyco_hydro_65N	e4ktrG1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
G	Glyco_hydro_65C	e4ktrG3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
G	UNK_F_TYPE	e4ktrG2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)
H	Glyco_hydro_65N	e4ktrH1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_65N	ECOD (1.6)
H	Glyco_hydro_65C	e4ktrH3	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: Glycoside hydrolase family 127 middle domain-related (From Topology)	T: Glycoside hydrolase family 127 middle domain-related	F: Glyco_hydro_65C	ECOD (1.6)
H	UNK_F_TYPE	e4ktrH2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: UNK_F_TYPE	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
B	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
B	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
E	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
E	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
E	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
A	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
A	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
A	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
C	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
C	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
C	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
D	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
D	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
D	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
F	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
F	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
F	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
G	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
G	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
G	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)
H	2.70.98.40	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
H	2.60.420.10	Mainly Beta	Sandwich	Maltose phosphorylase, domain 3	Maltose phosphorylase, domain 3	CATH (4.3.0)
H	1.50.10.10	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF03636	Glycosyl hydrolase family 65, N-terminal domain (Glyco_hydro_65N)	Glycosyl hydrolase family 65, N-terminal domain	-	Family
A, B, C, D, E A, B, C, D, E, F, G, H	PF03632	Glycosyl hydrolase family 65 central catalytic domain (Glyco_hydro_65m)	Glycosyl hydrolase family 65 central catalytic domain	-	Repeat
A, B, C, D, E A, B, C, D, E, F, G, H	PF03633	Glycosyl hydrolase family 65, C-terminal domain (Glyco_hydro_65C)	Glycosyl hydrolase family 65, C-terminal domain	This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyses the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucos ...	This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyses the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The C-terminal domain forms a two layered jelly roll motif. This domain is situated at the base of the catalytic domain, however its function remains unknown [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	Glycoside hydrolase family 65 central catalytic	binding carbohydrate binding glycosyltransferase activity transferase activity catalytic activity hydrolase activity, acting on glycosyl bonds glucosidase activity catalytic activity, acting on a protein protein-glucosylgalactosylhydroxylysine glucosidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity alpha,alpha-trehalase activity trehalase activity cation binding binding carbohydrate binding glycosyltransferase activity transferase activity catalytic activity hydrolase activity, acting on glycosyl bonds glucosidase activity catalytic activity, acting on a protein protein-glucosylgalactosylhydroxylysine glucosidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity alpha,alpha-trehalase activity trehalase activity cation binding ion binding metal ion binding small molecule binding	carbohydrate metabolic process trehalose catabolic process catabolic process trehalose metabolic process oligosaccharide catabolic process oligosaccharide metabolic process carbohydrate catabolic process primary metabolic process disaccharide metabolic process metabolic process disaccharide catabolic process	fungal-type cell wall cell periphery external encapsulating structure cell wall cellular anatomical entity intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR037018	Glycoside hydrolase family 65, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR008928	Six-hairpin glycosidase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR005195	Glycoside hydrolase, family 65, central catalytic	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR005194	Glycoside hydrolase family 65, C-terminal	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR012341	Six-hairpin glycosidase-like superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR017045	Maltose phosphorylase/glycosyl hydrolase/vacuolar acid trehalase	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR011013	Galactose mutarotase-like domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR005196	Glycoside hydrolase, family 65, N-terminal	Domain