5E8J

Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with RAM

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Methyltransf_11_3	e5e8jA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_3	ECOD (1.6)
B	Methyltransf_11_3	e5e8jB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C, D	PF15320	mRNA cap methylation, RNMT-activating mini protein (RAM)	mRNA cap methylation, RNMT-activating mini protein	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	mRNA cap guanine-N7 methyltransferase	RNA methyltransferase activity catalytic activity, acting on RNA transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid transferase activity S-adenosylmethionine-dependent methyltransferase activity mRNA 5'-cap (guanine-N7-)-methyltransferase activity N-methyltransferase activity methyltransferase activity catalytic activity mRNA methyltransferase activity RNA binding binding organic cyclic compound binding RNA methyltransferase activity catalytic activity, acting on RNA transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid transferase activity S-adenosylmethionine-dependent methyltransferase activity mRNA 5'-cap (guanine-N7-)-methyltransferase activity N-methyltransferase activity methyltransferase activity catalytic activity mRNA methyltransferase activity RNA binding binding organic cyclic compound binding nucleic acid binding	7-methylguanosine mRNA capping cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process 7-methylguanosine RNA capping RNA capping gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process mRNA processing mRNA metabolic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process 7-methylguanosine mRNA capping cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process 7-methylguanosine RNA capping RNA capping gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process mRNA processing mRNA metabolic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process nucleic acid biosynthetic process biosynthetic process RNA 5'-end processing metabolic process cellular process RNA processing macromolecule metabolic process response to peptide response to stimulus response to chemical response to cytokine response to leukemia inhibitory factor cellular response to cytokine stimulus cellular response to leukemia inhibitory factor	protein-containing complex receptor complex intracellular anatomical structure catalytic complex methyltransferase complex mRNA cap methyltransferase RNMT:RAMAC complex transferase complex cellular anatomical entity membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen protein-containing complex receptor complex intracellular anatomical structure catalytic complex methyltransferase complex mRNA cap methyltransferase RNMT:RAMAC complex transferase complex cellular anatomical entity membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen fibrillar center organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle nucleoplasm nuclear protein-containing complex mRNA capping enzyme complex
C, D	RNMT-activating mini protein	molecular function activator activity molecular function regulator activity enzyme regulator activity enzyme activator activity RNA binding binding organic cyclic compound binding nucleic acid binding	methylation metabolic process 7-methylguanosine mRNA capping cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process 7-methylguanosine RNA capping RNA capping gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process mRNA processing mRNA metabolic process methylation metabolic process 7-methylguanosine mRNA capping cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process 7-methylguanosine RNA capping RNA capping gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process mRNA processing mRNA metabolic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process nucleic acid biosynthetic process biosynthetic process RNA 5'-end processing cellular process RNA processing macromolecule metabolic process RNA (guanine-N7)-methylation RNA 5'-cap (guanine-N7)-methylation macromolecule methylation RNA modification macromolecule modification RNA methylation	protein-containing complex intracellular anatomical structure catalytic complex methyltransferase complex mRNA cap methyltransferase RNMT:RAMAC complex transferase complex cellular anatomical entity membrane-bounded organelle intracellular organelle organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle mRNA capping enzyme complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B	IPR016899	mRNA cap guanine-N7 methyltransferase, eukaryotes	Family
A, B	IPR039753	mRNA cap guanine-N7 methyltransferase	Family
A, B	IPR004971	mRNA (guanine-N(7))-methyltransferase domain	Domain
C, D	IPR028271	RNA guanine-N7 methyltransferase activating subunit	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: O43148	bone disease medulloblastoma large cell medulloblastoma ovarian cancer malignant mesothelioma spina bifida
C, D	Pharos: Q9BTL3	chronic obstructive pulmonary disease

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.