Annotations: 5FF4

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Radical_SAM	e5ff4A1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Radical_SAM	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF04055	Radical SAM superfamily (Radical_SAM)	Radical SAM superfamily	Radical SAM proteins catalyse diverse reactions, including unusual methylations, isomerisation, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	[FeFe] hydrogenase maturase subunit HydE	oxidoreductase activity catalytic activity iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding 4 iron, 4 sulfur cluster binding cation binding ion binding metal ion binding transferase activity	water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular biosynthetic process cellular metabolic process vitamin biosynthetic process vitamin metabolic process biosynthetic process small molecule metabolic process metabolic process cellular process water-soluble vitamin metabolic process sulfur compound biosynthetic process sulfur compound metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR007197	Radical SAM	Domain
A	IPR034422	HydE/PylB-like	Family
A	IPR006638	Elp3/MiaA/NifB-like, radical SAM core domain	Domain
A	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A	IPR010722	Biotin and thiamin synthesis-associated domain	Domain
A	IPR024021	[FeFe]-hydrogenase maturation HydE, radical SAM	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.