6C12

SDHA-SDHE complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth B]	d6c12b3	All alpha proteins	Spectrin repeat-like	Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
A [auth B]	d6c12b1	Alpha and beta proteins (a/b)	FAD/NAD(P)-binding domain	FAD/NAD(P)-binding domain	Succinate dehydrogenase/fumarate reductase flavoprotein N-terminal domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
A [auth B]	d6c12b2	Alpha and beta proteins (a+b)	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
C [auth A]	d6c12a3	All alpha proteins	Spectrin repeat-like	Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
C [auth A]	d6c12a1	Alpha and beta proteins (a/b)	FAD/NAD(P)-binding domain	FAD/NAD(P)-binding domain	Succinate dehydrogenase/fumarate reductase flavoprotein N-terminal domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
C [auth A]	d6c12a2	Alpha and beta proteins (a+b)	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	Succinate dehydogenase	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
B [auth D]	d6c12d_	All alpha proteins	YgfY-like	YgfY-like	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
D [auth C]	d6c12c_	All alpha proteins	YgfY-like	YgfY-like	automated matches	automated matches	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	Succinate dehydrogenase/fumarate reductase domain-like	8056067	3000054	SCOP2B (2022-06-29)
A [auth B]	SCOP2B Superfamily	L-aspartate oxidase C-terminal domain-like	8056068	3000021	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Succinate dehydrogenase/fumarate reductase domain-like	8056067	3000054	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	L-aspartate oxidase C-terminal domain-like	8056068	3000021	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	Succ_DH_flav_C	e6c12B3	A: alpha bundles	X: Spectrin repeat-like	H: Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain (From Topology)	T: Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	F: Succ_DH_flav_C	ECOD (1.6)
A [auth B]	FAD_binding_2	e6c12B1	A: a+b complex topology	X: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain (From Topology)	H: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain (From Topology)	T: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	F: FAD_binding_2	ECOD (1.6)
A [auth B]	FAD_binding_3_1st	e6c12B2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: FAD/NAD(P)-binding domain	F: FAD_binding_3_1st	ECOD (1.6)
C [auth A]	Succ_DH_flav_C	e6c12A3	A: alpha bundles	X: Spectrin repeat-like	H: Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain (From Topology)	T: Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain	F: Succ_DH_flav_C	ECOD (1.6)
C [auth A]	FAD_binding_2	e6c12A1	A: a+b complex topology	X: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain (From Topology)	H: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain (From Topology)	T: Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain	F: FAD_binding_2	ECOD (1.6)
C [auth A]	FAD_binding_3_1st	e6c12A2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: FAD/NAD(P)-binding domain	F: FAD_binding_3_1st	ECOD (1.6)
B [auth D]	Sdh5	e6c12D1	A: alpha arrays	X: YgfY-like (DUP399, Pfam03937) (From Topology)	H: YgfY-like (DUP399, Pfam03937) (From Topology)	T: YgfY-like (DUP399, Pfam03937)	F: Sdh5	ECOD (1.6)
D [auth C]	Sdh5	e6c12C1	A: alpha arrays	X: YgfY-like (DUP399, Pfam03937) (From Topology)	H: YgfY-like (DUP399, Pfam03937) (From Topology)	T: YgfY-like (DUP399, Pfam03937)	F: Sdh5	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B [auth D]	1.10.150.250	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
D [auth C]	1.10.150.250	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], C [auth A]	PF02910	Fumarate reductase flavoprotein C-term (Succ_DH_flav_C)	Fumarate reductase flavoprotein C-term	This family contains fumarate reductases, succinate dehydrogenases and L-aspartate oxidases.	Domain
A [auth B], C [auth A]	PF00890	FAD binding domain (FAD_binding_2)	FAD binding domain	-	Family
B [auth D], D [auth C]	PF03937	Flavinator of succinate dehydrogenase (Sdh5)	Flavinator of succinate dehydrogenase	This family includes the highly conserved mitochondrial and bacterial proteins Sdh5/SDHAF2/SdhE. Both yeast and human Sdh5/SDHAF2 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex, a component of both the electron trans ...	This family includes the highly conserved mitochondrial and bacterial proteins Sdh5/SDHAF2/SdhE. Both yeast and human Sdh5/SDHAF2 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex, a component of both the electron transport chain and the tricarboxylic acid cycle. Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination (incorporation of the flavin adenine dinucleotide cofactor). Mutational inactivation of Sdh5 confers tumor susceptibility in humans [1]. Bacterial homologues of Sdh5, termed SdhE, are functionally conserved being required for the flavinylation of SdhA and succinate dehydrogenase activity. Like Sdh5, SdhE interacts with SdhA. Furthermore, SdhE was characterised as a FAD co-factor chaperone that directly binds FAD to facilitate the flavinylation of SdhA. Phylogenetic analysis demonstrates that SdhE/Sdh5 proteins evolved only once in an ancestral alpha-proteobacteria prior to the evolution of the mitochondria and now remain in subsequent descendants including eukaryotic mitochondria and the alpha, beta and gamma proteobacteria [2]. This family was previously annotated in Pfam as being a divergent TPR repeat but structural evidence has indicated this is not true. The E. coli protein, YgfY also acts as the antitoxin to the membrane-bound toxin family Cpta, Pfam:PF13166, whose E. coli member YgfX, expressed from the same operon as YgfY [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], C [auth A]	Succinate dehydrogenase flavoprotein subunit	oxidoreductase activity succinate dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors catalytic activity flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor oxidoreductase activity succinate dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors catalytic activity flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor succinate dehydrogenase (quinone) activity electron transfer activity	aerobic respiration cellular respiration tricarboxylic acid cycle energy derivation by oxidation of organic compounds metabolic process cellular process primary metabolic process generation of precursor metabolites and energy aerobic electron transport chain oxidative phosphorylation respiratory electron transport chain electron transport chain anaerobic respiration	cellular anatomical structure membrane cell periphery plasma membrane
B [auth D], D [auth C]	FAD assembly factor SdhE	-	cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis respiratory chain complex II assembly succinate metabolic process oxoacid metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process small molecule metabolic process metabolic process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis respiratory chain complex II assembly succinate metabolic process oxoacid metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process small molecule metabolic process metabolic process organic acid metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], C [auth A]	IPR015939	Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal	Domain
A [auth B], C [auth A]	IPR003952	Fumarate reductase/succinate dehydrogenase, FAD-binding site	Binding Site
A [auth B], C [auth A]	IPR037099	Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamily	Homologous Superfamily
A [auth B], C [auth A]	IPR036188	FAD/NAD(P)-binding domain superfamily	Homologous Superfamily
A [auth B], C [auth A]	IPR003953	FAD-dependent oxidoreductase 2, FAD binding domain	Domain
A [auth B], C [auth A]	IPR030664	FAD-dependent oxidoreductase SdhA/FrdA/AprA	Family
A [auth B], C [auth A]	IPR027477	Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamily	Homologous Superfamily
A [auth B], C [auth A]	IPR014006	Succinate dehydrogenase/fumarate reductase, flavoprotein subunit	Family
A [auth B], C [auth A]	IPR011281	Succinate dehydrogenase, flavoprotein subunit	Family
B [auth D], D [auth C]	IPR036714	Flavinator of succinate dehydrogenase superfamily	Homologous Superfamily
B [auth D], D [auth C]	IPR050531	SdhE FAD assembly factor	Family
B [auth D], D [auth C]	IPR005631	Flavinator of succinate dehydrogenase	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.