6H9E

Structure of glutamate mutase reconstituted with homo-coenzyme B12

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6h9ea_	Alpha and beta proteins (a/b)	Flavodoxin-like	Cobalamin (vitamin B12)-binding domain	Cobalamin (vitamin B12)-binding domain	Glutamate mutase, small subunit	(Clostridium cochlearium ) [TaxId: 1494 ],	SCOPe (2.08)
C	d6h9ec_	Alpha and beta proteins (a/b)	Flavodoxin-like	Cobalamin (vitamin B12)-binding domain	Cobalamin (vitamin B12)-binding domain	Glutamate mutase, small subunit	(Clostridium cochlearium ) [TaxId: 1494 ],	SCOPe (2.08)
B	d6h9eb_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Cobalamin (vitamin B12)-dependent enzymes	Glutamate mutase, large subunit	Glutamate mutase, large subunit	(Clostridium cochlearium ) [TaxId: 1494 ],	SCOPe (2.08)
D	d6h9ed_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Cobalamin (vitamin B12)-dependent enzymes	Glutamate mutase, large subunit	Glutamate mutase, large subunit	(Clostridium cochlearium ) [TaxId: 1494 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Cobalamin (vitamin B12)-binding domain	8044100	3001260	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Cobalamin (vitamin B12)-binding domain	8044100	3001260	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Cobalamin (vitamin B12)-dependent enzymes	8043856	3000609	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Cobalamin (vitamin B12)-dependent enzymes	8043856	3000609	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	B12-binding	e6h9eA1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: B12-binding	ECOD (1.6)
C	B12-binding	e6h9eC1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: B12-binding	ECOD (1.6)
B	Met_asp_mut_E	e6h9eB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Met_asp_mut_E	ECOD (1.6)
D	Met_asp_mut_E	e6h9eD1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Met_asp_mut_E	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.280	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Cobalamin-binding domain	CATH (utative)
C	3.40.50.280	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Cobalamin-binding domain	CATH (utative)
B	3.20.20.240	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Methylmalonyl-CoA mutase	CATH (utative)
B	3.90.970.10	Alpha Beta	Alpha-Beta Complex	Glutamate mutase, C-terminal domain		CATH (utative)
D	3.20.20.240	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Methylmalonyl-CoA mutase	CATH (utative)
D	3.90.970.10	Alpha Beta	Alpha-Beta Complex	Glutamate mutase, C-terminal domain		CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF02310	B12 binding domain (B12-binding)	B12 binding domain	This domain binds to B12 (adenosylcobamide)[1-3], it is found in several enzymes, such as glutamate mutase Swiss:Q05488, methionine synthase Swiss:Q99707 and methylmalonyl-CoA mutase Swiss:P22033. It contains a conserved DxHxxGx(41)SxVx(26)GG motif, ...	This domain binds to B12 (adenosylcobamide)[1-3], it is found in several enzymes, such as glutamate mutase Swiss:Q05488, methionine synthase Swiss:Q99707 and methylmalonyl-CoA mutase Swiss:P22033. It contains a conserved DxHxxGx(41)SxVx(26)GG motif, which is important for B12 binding [2].	Domain
B, D	PF06368	Methylaspartate mutase E chain (MutE) (Met_asp_mut_E)	Methylaspartate mutase E chain (MutE)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	Glutamate mutase sigma subunit	vitamin binding binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding small molecule binding isomerase activity methylaspartate mutase activity catalytic activity intramolecular transferase activity cation binding ion binding metal ion binding	catabolic process oxoacid metabolic process glutamine family amino acid catabolic process amino acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process catabolic process oxoacid metabolic process glutamine family amino acid catabolic process amino acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process glutamate catabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process L-amino acid catabolic process primary metabolic process glutamate catabolic process via L-citramalate L-amino acid metabolic process proteinogenic amino acid metabolic process alpha-amino acid catabolic process proteinogenic amino acid catabolic process metabolic process cellular process small molecule catabolic process energy derivation by oxidation of organic compounds anaerobic glutamate catabolic process fermentation nitrogenous compound fermentation generation of precursor metabolites and energy anaerobic amino acid catabolic process	-
B, D	Glutamate mutase epsilon subunit	vitamin binding binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding small molecule binding isomerase activity methylaspartate mutase activity catalytic activity intramolecular transferase activity	catabolic process oxoacid metabolic process glutamine family amino acid catabolic process amino acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process catabolic process oxoacid metabolic process glutamine family amino acid catabolic process amino acid metabolic process organic acid metabolic process dicarboxylic acid catabolic process organic acid catabolic process alpha-amino acid metabolic process glutamine family amino acid metabolic process cellular catabolic process carboxylic acid catabolic process glutamate metabolic process small molecule metabolic process amino acid catabolic process glutamate catabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process L-amino acid catabolic process primary metabolic process glutamate catabolic process via L-citramalate L-amino acid metabolic process proteinogenic amino acid metabolic process alpha-amino acid catabolic process proteinogenic amino acid catabolic process metabolic process cellular process small molecule catabolic process energy derivation by oxidation of organic compounds anaerobic glutamate catabolic process fermentation nitrogenous compound fermentation generation of precursor metabolites and energy anaerobic amino acid catabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR036724	Cobalamin-binding domain superfamily	Homologous Superfamily
A, C	IPR006158	Cobalamin (vitamin B12)-binding domain	Domain
A, C	IPR006394	Glutamate mutase sigma subunit	Family
B, D	IPR014714	Glutamate mutase E subunit, C-terminal domain superfamily	Homologous Superfamily
B, D	IPR016176	Cobalamin (vitamin B12)-dependent enzyme, catalytic	Homologous Superfamily
B, D	IPR006396	Glutamate mutase epsilon subunit	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.