Annotations: 6R6T

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	MmgE_PrpD_1st	e6r6tA2	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: MmgE_PrpD_1st	ECOD (1.6)
A	MmgE_PrpD_2nd	e6r6tA1	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: MmgE_PrpD_2nd	ECOD (1.6)
B	MmgE_PrpD_1st	e6r6tB1	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: MmgE_PrpD_1st	ECOD (1.6)
B	MmgE_PrpD_2nd	e6r6tB2	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: MmgE_PrpD_2nd	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF19305	MmgE/PrpD C-terminal domain (MmgE_PrpD_C)	MmgE/PrpD C-terminal domain	This entry represents the C-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle. This domain is related to the serine dehydratase b ...	This entry represents the C-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle. This domain is related to the serine dehydratase beta chain.	Domain
A, B	PF03972	MmgE/PrpD N-terminal domain (MmgE_PrpD_N)	MmgE/PrpD N-terminal domain	This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle [1]. This enzyme consists of two domains: a large d ...	This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle [1]. This enzyme consists of two domains: a large domain with an all-helical fold and a smaller domain that folds into an alpha/beta domain [2]. Cis-aconitic acid decarboxylase (CAD) shares high identity with proteins of the MmgE/PrpD family [3,4]. Citrate/2-methylcitrate dehydratase from Bacillus subtilis is involved in the tricarboxylic acid (TCA) and methylcitric acid cycles as it has both 2-methylcitrate dehydratase and citrate dehydratase activities [5]. This entry represents the N-terminal domain of 2-methylcitrate dehydratase PrpD, which has an all-helical fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Cis-aconitate decarboxylase	aconitate decarboxylase activity carboxy-lyase activity lyase activity catalytic activity carbon-carbon lyase activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity	cellular response to stimulus positive regulation of response to biotic stimulus immune response-regulating cell surface receptor signaling pathway regulation of cellular process response to stimulus pattern recognition receptor signaling pathway negative regulation of biological process regulation of signal transduction response to biotic stimulus cell communication immune response-activating signaling pathway positive regulation of immune response regulation of immune system process signal transduction cellular response to stimulus positive regulation of response to biotic stimulus immune response-regulating cell surface receptor signaling pathway regulation of cellular process response to stimulus pattern recognition receptor signaling pathway negative regulation of biological process regulation of signal transduction response to biotic stimulus cell communication immune response-activating signaling pathway positive regulation of immune response regulation of immune system process signal transduction positive regulation of immune system process cell surface toll-like receptor signaling pathway innate immune response-activating signaling pathway regulation of biological process immune response response to other organism immune response-activating cell surface receptor signaling pathway regulation of innate immune response response to external stimulus regulation of toll-like receptor 4 signaling pathway regulation of response to stimulus defense response to other organism regulation of pattern recognition receptor signaling pathway positive regulation of defense response activation of innate immune response regulation of immune response activation of immune response regulation of defense response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of response to external stimulus negative regulation of signaling signaling positive regulation of biological process innate immune response activating cell surface receptor signaling pathway negative regulation of cell communication positive regulation of response to stimulus positive regulation of response to external stimulus immune response-regulating signaling pathway regulation of response to stress response to stress negative regulation of cellular process innate immune response response to external biotic stimulus negative regulation of toll-like receptor 4 signaling pathway cell surface pattern recognition receptor signaling pathway regulation of signaling positive regulation of innate immune response cell surface receptor signaling pathway negative regulation of response to stimulus defense response biological regulation defense response to symbiont negative regulation of signal transduction negative regulation of immune system process immune system process toll-like receptor 4 signaling pathway regulation of cell communication cellular process multicellular organismal process negative regulation of type I interferon production cytokine production regulation of cytokine production regulation of multicellular organismal process negative regulation of macromolecule metabolic process regulation of biosynthetic process type I interferon production negative regulation of metabolic process negative regulation of cytokine production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process negative regulation of multicellular organismal process regulation of metabolic process regulation of type I interferon production regulation of humoral immune response humoral immune response antimicrobial humoral response positive regulation of humoral immune response regulation of antimicrobial humoral response positive regulation of antimicrobial humoral response response to ketone response to lipid response to endogenous stimulus cellular response to organic cyclic compound response to progesterone response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to endogenous stimulus cellular response to chemical stimulus response to hormone cellular response to progesterone stimulus response to chemical response to steroid hormone cellular response to lipid cellular response to ketone cellular response to steroid hormone stimulus response to organic cyclic compound cellular response to hormone stimulus negative regulation of response to biotic stimulus negative regulation of immune response negative regulation of response to external stimulus negative regulation of defense response negative regulation of innate immune response negative regulation of inflammatory response regulation of inflammatory response inflammatory response cellular response to lipopolysaccharide cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to biotic stimulus response to bacterium response to molecule of bacterial origin cellular response to interferon-beta response to peptide response to interferon-beta response to cytokine cellular response to cytokine stimulus negative regulation of DNA-binding transcription factor activity RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process negative regulation of NF-kappaB transcription factor activity nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process negative regulation of molecular function primary metabolic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process regulation of molecular function regulation of DNA-binding transcription factor activity regulation of DNA-templated transcription developmental process multicellular organism development multi-multicellular organism process reproductive process anatomical structure development female pregnancy embryo implantation multi-organism reproductive process negative regulation of toll-like receptor 2 signaling pathway regulation of toll-like receptor 2 signaling pathway toll-like receptor 2 signaling pathway reactive oxygen species metabolic process positive regulation of reactive oxygen species metabolic process positive regulation of metabolic process positive regulation of cellular process regulation of reactive oxygen species metabolic process cellular response to tumor necrosis factor response to tumor necrosis factor response to type II interferon cellular response to type II interferon regulation of lipopolysaccharide-mediated signaling pathway lipopolysaccharide-mediated signaling pathway system development tolerance induction to lipopolysaccharide tolerance induction immune system development negative regulation of lipopolysaccharide-mediated signaling pathway defense response to virus response to virus cellular response to interleukin-1 response to interleukin-1	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR036148	MmgE/PrpD superfamily	Homologous Superfamily
A, B	IPR005656	MmgE/PrpD	Family
A, B	IPR045336	MmgE/PrpD, N-terminal	Domain
A, B	IPR042188	MmgE/PrpD superfamily, domain 2	Homologous Superfamily
A, B	IPR042183	MmgE/PrpD superfamily, domain 1	Homologous Superfamily
A, B	IPR045337	MmgE/PrpD, C-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.