6VPL

TPX2 residues 7-20 fused to Aurora A residues 116-389 with C290 disulfide bonded to compound 7-80, and in complex with AMP-PNP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6vpla1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d6vpla2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d6vplb1	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d6vplb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ], Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Protein kinase-like (PK-like)	8042948	3000066	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Protein kinase-like (PK-like)	8042948	3000066	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Pkinase_Tyr	e6vplA1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
B	Pkinase_Tyr	e6vplB1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	TPX2 fragment - Aurora A kinase domain fusion	protein binding binding kinase binding enzyme binding protein kinase binding molecular adaptor activity importin-alpha family protein binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding protein binding binding kinase binding enzyme binding protein kinase binding molecular adaptor activity importin-alpha family protein binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding ubiquitin-like protein ligase binding protein serine/threonine/tyrosine kinase activity protein heterodimerization activity protein dimerization activity protein serine kinase activity protein serine/threonine kinase activity molecular function activator activity molecular function regulator activity histone H3 kinase activity histone modifying activity histone H3S10 kinase activity histone kinase activity	cellular process cell cycle mitotic cell cycle cell division apoptotic process cell death programmed cell death regulation of cellular process positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process cellular process cell cycle mitotic cell cycle cell division apoptotic process cell death programmed cell death regulation of cellular process positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of macromolecule metabolic process phosphorus metabolic process protein modification process positive regulation of protein phosphorylation regulation of biological process protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation cellular metabolic process regulation of protein metabolic process regulation of cellular metabolic process primary metabolic process regulation of molecular function positive regulation of kinase activity regulation of kinase activity activation of protein kinase activity macromolecule modification regulation of phosphorus metabolic process metabolic process macromolecule metabolic process regulation of metabolic process regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of molecular function positive regulation of protein modification process protein metabolic process positive regulation of protein kinase activity positive regulation of cellular process positive regulation of transferase activity biological regulation positive regulation of catalytic activity regulation of protein kinase activity regulation of macromolecule metabolic process regulation of phosphorylation regulation of cytoskeleton organization regulation of cellular component organization microtubule cytoskeleton organization regulation of mitotic spindle organization cellular component organization cellular component organization or biogenesis regulation of cell cycle microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process regulation of cell cycle process regulation of microtubule-based process regulation of spindle organization regulation of organelle organization mitotic spindle organization organelle organization mitotic cell cycle process microtubule-based process cytoskeleton organization regulation of microtubule cytoskeleton organization non-membrane-bounded organelle assembly cellular component biogenesis cellular component assembly mitotic sister chromatid segregation chromosome segregation mitotic nuclear division organelle assembly organelle fission chromosome organization sister chromatid segregation spindle assembly mitotic spindle assembly nuclear division nuclear chromosome segregation regulation of microtubule polymerization or depolymerization negative regulation of microtubule depolymerization negative regulation of biological process protein-containing complex disassembly regulation of protein-containing complex disassembly microtubule polymerization or depolymerization regulation of microtubule depolymerization negative regulation of protein-containing complex disassembly negative regulation of cellular process negative regulation of supramolecular fiber organization cellular component disassembly negative regulation of cellular component organization negative regulation of microtubule polymerization or depolymerization protein-containing complex organization regulation of protein depolymerization negative regulation of cytoskeleton organization negative regulation of protein depolymerization negative regulation of organelle organization microtubule depolymerization regulation of supramolecular fiber organization supramolecular fiber organization protein depolymerization protein polymerization microtubule polymerization microtubule nucleation protein-containing complex assembly regulation of protein binding negative regulation of protein binding negative regulation of binding regulation of binding negative regulation of molecular function localization centrosome localization microtubule organizing center localization positive regulation of proteasomal protein catabolic process catabolic process proteolysis involved in protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process regulation of ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-dependent protein catabolic process regulation of proteolysis regulation of proteolysis involved in protein catabolic process modification-dependent macromolecule catabolic process proteolysis proteasomal protein catabolic process protein catabolic process regulation of catabolic process positive regulation of protein catabolic process positive regulation of proteolysis involved in protein catabolic process positive regulation of catabolic process regulation of proteasomal ubiquitin-dependent protein catabolic process modification-dependent protein catabolic process positive regulation of proteolysis regulation of proteasomal protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process regulation of protein catabolic process positive regulation of organelle organization positive regulation of cellular component organization regulation of mitotic nuclear division positive regulation of mitotic nuclear division regulation of mitotic cell cycle positive regulation of cell cycle process positive regulation of nuclear division positive regulation of cell cycle regulation of nuclear division protein autophosphorylation regulation of cytokinesis cytokinesis regulation of cell division regulation of protein stability regulation of biological quality response to stimulus response to stress response to wounding cell cycle G2/M phase transition regulation of cell cycle phase transition regulation of G2/M transition of mitotic cell cycle G2/M transition of mitotic cell cycle regulation of cell cycle G2/M phase transition cell cycle phase transition regulation of mitotic cell cycle phase transition mitotic cell cycle phase transition peptidyl-serine modification peptidyl-serine phosphorylation peptidyl-amino acid modification plasma membrane bounded cell projection organization organelle disassembly cell projection organization cilium disassembly cilium organization protein localization to microtubule cytoskeleton protein localization cellular localization protein localization to microtubule organizing center protein localization to cytoskeleton protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to centrosome negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death negative regulation of apoptotic process meiotic chromosome segregation sexual reproduction reproductive process female gamete generation meiotic cell cycle process gamete generation meiosis I female meiotic nuclear division spindle assembly involved in female meiosis I meiotic nuclear division meiotic cell cycle multicellular organismal reproductive process meiotic spindle organization meiotic spindle assembly meiosis I cell cycle process female meiosis I spindle assembly involved in female meiosis multicellular organismal process cellular developmental process developmental growth plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell growth cell morphogenesis neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development system development developmental process neuron projection extension developmental cell growth growth nervous system development developmental growth involved in morphogenesis generation of neurons anatomical structure morphogenesis neuron development gland development animal organ development animal organ regeneration liver development regeneration hepaticobiliary system development liver regeneration anterior/posterior axis specification anterior/posterior pattern specification pattern specification process regionalization axis specification regulation of developmental process positive regulation of developmental process regulation of anatomical structure morphogenesis mitochondrion organization mitochondrial fission regulation of mitochondrial fission regulation of mitochondrion organization positive regulation of mitochondrial fission microtubule organizing center organization regulation of centrosome cycle centrosome cycle cellular response to stimulus regulation of signaling signaling regulation of signal transduction signal transduction by p53 class mediator intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus regulation of signal transduction by p53 class mediator regulation of cell communication signal transduction positive regulation of oocyte maturation regulation of reproductive process regulation of oocyte maturation cellular process involved in reproduction in multicellular organism regulation of cell differentiation positive regulation of cell maturation developmental process involved in reproduction regulation of cell development oocyte development positive regulation of cell differentiation cell maturation regulation of cell maturation positive regulation of reproductive process oogenesis positive regulation of cell development developmental maturation anatomical structure maturation germ cell development oocyte maturation oocyte differentiation cellular biosynthetic process negative regulation of cellular metabolic process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process gene expression macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process positive regulation of mitotic cell cycle centrosome separation mitotic centrosome separation	intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle cytoplasm cytosol somatodendritic compartment axon intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle cytoplasm cytosol somatodendritic compartment axon cell body cell projection neuron projection neuronal cell body axon hillock plasma membrane bounded cell projection mitotic spindle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen intercellular bridge polymeric cytoskeletal fiber supramolecular fiber microtubule supramolecular polymer supramolecular complex spindle midzone cell periphery basal part of cell membrane plasma membrane basal plasma membrane plasma membrane region basolateral plasma membrane synapse cell junction glutamatergic synapse perinuclear region of cytoplasm mitotic spindle pole cilium ciliary basal body microtubule organizing center midbody germinal vesicle germ cell nucleus female germ cell nucleus centrosome centriole protein-containing complex chromosome passenger complex microtubule associated complex chromosome chromosomal region kinetochore chromosome, centromeric region condensed chromosome, centromeric region condensed chromosome spindle pole centrosome pronucleus postsynaptic density postsynapse neuron to neuron synapse postsynaptic specialization asymmetric synapse meiotic spindle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR015128	Aurora-A binding	Domain
A, B	IPR027329	TPX2, C-terminal	Domain
A, B	IPR027330	TPX2 central domain	Domain
A, B	IPR009675	TPX2	Family
A, B	IPR000719	Protein kinase domain	Domain
A, B	IPR017441	Protein kinase, ATP binding site	Binding Site
A, B	IPR030616	Aurora kinase-like	Family
A, B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B	IPR030611	Aurora kinase A	Domain
A, B	IPR008271	Serine/threonine-protein kinase, active site	Active Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: O14965	malignant pancreatic neoplasm non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm adrenal cortex carcinoma breast cancer ductal breast carcinoma in situ hepatocellular carcinoma invasive ductal breast carcinoma lung cancer malignant glioma medulloblastoma ovarian cancer primitive neuroectodermal tumor atopic eczema breast carcinoma malignant pancreatic neoplasm non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm adrenal cortex carcinoma breast cancer ductal breast carcinoma in situ hepatocellular carcinoma invasive ductal breast carcinoma lung cancer malignant glioma medulloblastoma ovarian cancer primitive neuroectodermal tumor atopic eczema breast carcinoma endometriosis IgA glomerulonephritis large cell medulloblastoma malignant colon neoplasm malignant mesothelioma nasopharyngeal carcinoma pancreatic intraductal papillary-mucinous carcinoma psoriasis atypical teratoid rhabdoid tumor colorectal cancer glioblastoma interstitial cystitis lung adenocarcinoma malignant ependymoma cancer colorectal cancer breast carcinoma melanoma neuroblastoma peripheral neuropathy prostate cancer aneuploidy breast cancer Chromosomal Instability kidney cancer peripheral nervous system disease interstitial cystitis psoriasis kidney neoplasm lung cancer malignant glioma adrenal cortex carcinoma malignant pancreatic neoplasm atypical teratoid rhabdoid tumor metastatic melanoma non-small cell lung carcinoma cutaneous lupus erythematosus pancreatic intraductal papillary-mucinous carcinoma endometriosis hepatocellular carcinoma invasive ductal breast carcinoma thrombocytopenia due to immune destruction large cell medulloblastoma malignant colon neoplasm medulloblastoma nasopharyngeal carcinoma colorectal carcinoma osteosarcoma cutaneous melanoma pancreatic intraductal papillary-mucinous neoplasm esophageal adenocarcinoma primitive neuroectodermal tumor IgA glomerulonephritis lung adenocarcinoma malignant ependymoma atopic eczema colorectal adenocarcinoma ovarian cancer ductal breast carcinoma in situ glioblastoma prostate adenocarcinoma

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, B	TPO	Parent Component: THR RESID: AA0038 PSI-MOD : O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.