6WFJ

Crystal structures of human E-NPP 1: apo

External Resource: Annotation

Domain Annotation: ECOD Classification
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth AcA]	Phosphodiest_C	e6wfjAcA5	A: a+b two layers	X: Alpha-beta plaits	H: Phosphonoacetate hydrolase insertion domain (From Topology)	T: Phosphonoacetate hydrolase insertion domain	F: Phosphodiest_C	ECOD (1.6)
A [auth AcA]	Phosphodiest_N	e6wfjAcA3	A: a/b three-layered sandwiches	X: Alkaline phosphatase-like (From Topology)	H: Alkaline phosphatase-like (From Topology)	T: Alkaline phosphatase-like	F: Phosphodiest_N	ECOD (1.6)
A [auth AcA]	Endonuclease_NS	e6wfjAcA4	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: Endonuclease_NS	ECOD (1.6)
A [auth AcA]	Somatomedin_B	e6wfjAcA2	A: few secondary structure elements	X: Somatomedin B domain (From Topology)	H: Somatomedin B domain (From Topology)	T: Somatomedin B domain	F: Somatomedin_B	ECOD (1.6)
B [auth BcB]	Phosphodiest_C	e6wfjBcB3	A: a+b two layers	X: Alpha-beta plaits	H: Phosphonoacetate hydrolase insertion domain (From Topology)	T: Phosphonoacetate hydrolase insertion domain	F: Phosphodiest_C	ECOD (1.6)
B [auth BcB]	Phosphodiest_N	e6wfjBcB1	A: a/b three-layered sandwiches	X: Alkaline phosphatase-like (From Topology)	H: Alkaline phosphatase-like (From Topology)	T: Alkaline phosphatase-like	F: Phosphodiest_N	ECOD (1.6)
B [auth BcB]	Endonuclease_NS	e6wfjBcB4	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: Endonuclease_NS	ECOD (1.6)
B [auth BcB]	Somatomedin_B	e6wfjBcB5	A: few secondary structure elements	X: Somatomedin B domain (From Topology)	H: Somatomedin B domain (From Topology)	T: Somatomedin B domain	F: Somatomedin_B	ECOD (1.6)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth AcA], B [auth BcB]	Ectonucleotide pyrophosphatase/phosphodiesterase family member 1	binding organic cyclic compound binding nucleic acid binding pyrophosphatase activity nucleoside triphosphate diphosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity GTP diphosphatase activity hydrolase activity, acting on acid anhydrides catalytic activity protein binding signaling receptor binding insulin receptor binding phosphoric ester hydrolase activity binding organic cyclic compound binding nucleic acid binding pyrophosphatase activity nucleoside triphosphate diphosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity GTP diphosphatase activity hydrolase activity, acting on acid anhydrides catalytic activity protein binding signaling receptor binding insulin receptor binding phosphoric ester hydrolase activity hydrolase activity, acting on ester bonds phosphatase activity cargo receptor activity scavenger receptor activity phosphoric diester hydrolase activity 3',5'-cyclic-nucleotide phosphodiesterase activity 3',5'-cyclic-AMP phosphodiesterase activity cyclic-nucleotide phosphodiesterase activity nuclease activity phosphodiesterase I activity exonuclease activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ATP diphosphatase activity UTP diphosphatase activity polysaccharide binding carbohydrate binding cation binding calcium ion binding metal ion binding 3'-phosphoadenosine 5'-phosphosulfate binding sulfur compound binding dinucleotide phosphatase activity identical protein binding protein homodimerization activity protein dimerization activity transition metal ion binding zinc ion binding cyclic-GMP-AMP hydrolase activity	developmental process multicellular organismal process ossification bone mineralization animal organ development anatomical structure development biomineral tissue development tissue development phosphate ion homeostasis inorganic ion homeostasis chemical homeostasis homeostatic process regulation of biological process regulation of growth developmental process multicellular organismal process ossification bone mineralization animal organ development anatomical structure development biomineral tissue development tissue development phosphate ion homeostasis inorganic ion homeostasis chemical homeostasis homeostatic process regulation of biological process regulation of growth regulation of cellular component organization regulation of cellular process negative regulation of biological process biological regulation growth negative regulation of cell growth regulation of cell growth cell growth cellular component organization negative regulation of growth negative regulation of cellular process cellular process cellular component organization or biogenesis cellular response to stimulus cellular response to nitrogen compound response to stimulus negative regulation of signaling signaling response to peptide hormone regulation of signal transduction insulin receptor signaling pathway regulation of cellular response to insulin stimulus negative regulation of cell communication cell surface receptor protein tyrosine kinase signaling pathway cell communication cellular response to endogenous stimulus response to hormone signal transduction enzyme-linked receptor protein signaling pathway cellular response to hormone stimulus regulation of signaling cell surface receptor signaling pathway negative regulation of response to stimulus response to endogenous stimulus regulation of insulin receptor signaling pathway response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound negative regulation of signal transduction cellular response to oxygen-containing compound cellular response to chemical stimulus negative regulation of cellular response to insulin stimulus regulation of response to stimulus negative regulation of insulin receptor signaling pathway response to chemical response to insulin regulation of cell communication cellular response to insulin stimulus regulation of multicellular organismal development regulation of developmental process regulation of biomineral tissue development regulation of bone mineralization multicellular organism development regulation of multicellular organismal process regulation of ossification regulation of generation of precursor metabolites and energy negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of carbohydrate metabolic process negative regulation of glycogen metabolic process carbohydrate biosynthetic process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process energy reserve metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of glycogen metabolic process regulation of metabolic process regulation of glycogen biosynthetic process negative regulation of glycogen biosynthetic process glucan biosynthetic process energy derivation by oxidation of organic compounds cellular biosynthetic process regulation of glucan biosynthetic process regulation of polysaccharide metabolic process polysaccharide metabolic process glucan metabolic process regulation of carbohydrate metabolic process negative regulation of macromolecule metabolic process regulation of biosynthetic process carbohydrate metabolic process polysaccharide biosynthetic process glycogen metabolic process generation of precursor metabolites and energy regulation of macromolecule metabolic process glycogen biosynthetic process macromolecule biosynthetic process regulation of cellular biosynthetic process negative regulation of biosynthetic process regulation of polysaccharide biosynthetic process regulation of carbohydrate biosynthetic process nucleoside phosphate metabolic process catabolic process nucleoside triphosphate catabolic process organophosphate catabolic process nucleoside phosphate catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process small molecule metabolic process nucleoside triphosphate metabolic process phosphorus metabolic process intracellular phosphate ion homeostasis cellular homeostasis intracellular chemical homeostasis inorganic diphosphate transport localization transport establishment of localization inorganic anion transport fat cell differentiation cellular developmental process negative regulation of developmental process regulation of cell differentiation negative regulation of fat cell differentiation cell differentiation negative regulation of cell differentiation regulation of fat cell differentiation ribose phosphate metabolic process ATP metabolic process carbohydrate derivative metabolic process purine nucleotide metabolic process ribonucleotide metabolic process purine ribonucleoside triphosphate metabolic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process nucleotide metabolic process purine-containing compound metabolic process purine ribonucleotide metabolic process immune response immune system process pigment cell differentiation pigmentation developmental pigmentation melanocyte differentiation regulation of localization hexose transmembrane transport D-glucose import carbohydrate transport negative regulation of transport negative regulation of transmembrane transport negative regulation of D-glucose import regulation of D-glucose import regulation of transport negative regulation of D-glucose transmembrane transport transmembrane transport regulation of transmembrane transport D-glucose transmembrane transport regulation of D-glucose transmembrane transport monosaccharide transmembrane transport carbohydrate transmembrane transport regulation of protein modification process regulation of protein phosphorylation negative regulation of phosphate metabolic process regulation of phosphate metabolic process negative regulation of protein modification process protein metabolic process protein modification process negative regulation of protein metabolic process protein phosphorylation protein autophosphorylation phosphorylation regulation of protein metabolic process regulation of protein autophosphorylation negative regulation of protein phosphorylation macromolecule modification negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process negative regulation of phosphorylation negative regulation of protein autophosphorylation DNA-templated transcription regulation of RNA metabolic process negative regulation of smoothened signaling pathway negative regulation of hh target transcription factor activity regulation of nucleobase-containing compound metabolic process regulation of smoothened signaling pathway regulation of gene expression regulation of molecular function regulation of DNA-binding transcription factor activity regulation of DNA-templated transcription RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process gene expression smoothened signaling pathway nucleic acid metabolic process response to organophosphorus response to ATP response to purine-containing compound response to organic cyclic compound sequestering of triglyceride macromolecule localization lipid storage lipid localization maintenance of location nucleoside bisphosphate metabolic process purine nucleoside bisphosphate metabolic process ribonucleoside bisphosphate metabolic process oxoacid metabolic process organic acid metabolic process purine ribonucleoside bisphosphate metabolic process sulfur compound metabolic process 3'-phosphoadenosine 5'-phosphosulfate metabolic process negative regulation of biomineral tissue development negative regulation of bone mineralization negative regulation of ossification negative regulation of multicellular organismal process	membrane cellular anatomical entity cell periphery basal part of cell plasma membrane basal plasma membrane plasma membrane region basolateral plasma membrane vacuolar membrane membrane-bounded organelle intracellular anatomical structure lytic vacuole vacuole intracellular membrane-bounded organelle membrane cellular anatomical entity cell periphery basal part of cell plasma membrane basal plasma membrane plasma membrane region basolateral plasma membrane vacuolar membrane membrane-bounded organelle intracellular anatomical structure lytic vacuole vacuole intracellular membrane-bounded organelle intracellular organelle lytic vacuole membrane bounding membrane of organelle organelle lysosomal membrane cytoplasm organelle membrane lysosome extracellular region extracellular space cell surface

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth AcA], B [auth BcB]	IPR020821	ENPP1-3/EXOG-like, endonuclease/phosphodiesterase domain	Domain
A [auth AcA], B [auth BcB]	IPR017850	Alkaline-phosphatase-like, core domain superfamily	Homologous Superfamily
A [auth AcA], B [auth BcB]	IPR020436	Somatomedin B domain, chordata	Domain
A [auth AcA], B [auth BcB]	IPR036024	Somatomedin B-like domain superfamily	Homologous Superfamily
A [auth AcA], B [auth BcB]	IPR044929	DNA/RNA non-specific endonuclease superfamily	Homologous Superfamily
A [auth AcA], B [auth BcB]	IPR001212	Somatomedin B domain	Domain
A [auth AcA], B [auth BcB]	IPR002591	Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase	Family
A [auth AcA], B [auth BcB]	IPR044925	His-Me finger superfamily	Homologous Superfamily
A [auth AcA], B [auth BcB]	IPR001604	DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth AcA], B [auth BcB]	Pharos: P22413	type 2 diabetes mellitus hypophosphatemic rickets, autosomal recessive, 2 arterial calcification of infancy autosomal recessive inherited pseudoxanthoma elasticum hypopigmentation-punctate palmoplantar keratoderma syndrome obesity disorder ossification of the posterior longitudinal ligament of the spine arterial calcification, generalized, of infancy, 1 endometriosis lung cancer maturity-onset diabetes of the young ovarian cancer acquired metabolic disease pancreatic intraductal papillary-mucinous carcinoma pituitary cancer type 2 diabetes mellitus hypophosphatemic rickets, autosomal recessive, 2 arterial calcification of infancy autosomal recessive inherited pseudoxanthoma elasticum hypopigmentation-punctate palmoplantar keratoderma syndrome obesity disorder ossification of the posterior longitudinal ligament of the spine arterial calcification, generalized, of infancy, 1 endometriosis lung cancer maturity-onset diabetes of the young ovarian cancer acquired metabolic disease pancreatic intraductal papillary-mucinous carcinoma pituitary cancer Crohn disease Diabetes Mellitus, Non-Insulin-Dependent erythrokeratoderma intellectual disability lung carcinoma monogenic obesity osteogenesis imperfecta palmoplantar keratosis pancreatic intraductal papillary-mucinous neoplasm breast cancer rickets cystic fibrosis ulcerative colitis hypophosphatemia invasive ductal breast carcinoma malignant colon neoplasm osteosarcoma pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia subependymal giant cell astrocytoma diabetes mellitus hypophosphatemic rickets, autosomal recessive, 1

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A [auth AcA], B [auth BcB]	NEP	Parent Component: HIS RESID: AA0035 PSI-MOD : 1'-phospho-L-histidine MOD:00044

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.