7OBH

Crystal structure of 14-3-3 sigma in complex with NPM1 phosphopeptide and stabilizer Fusicoccin-A

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	14-3-3	e7obhA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: 14-3-3	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00244	14-3-3 protein (14-3-3)	14-3-3 protein	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	14-3-3 protein sigma	phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity molecular sequestering activity protein sequestering activity kinase binding enzyme binding protein kinase binding cell adhesion molecule binding cadherin binding identical protein binding	regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis positive regulation of cellular process protein localization localization cellular localization cellular macromolecule localization macromolecule localization regulation of localization regulation of protein localization negative regulation of biological process negative regulation of protein localization to plasma membrane negative regulation of cellular process regulation of protein localization to membrane negative regulation of protein localization protein localization to cell periphery protein localization to plasma membrane localization within membrane regulation of protein localization to cell periphery protein localization to membrane negative regulation of protein localization to membrane regulation of cellular localization negative regulation of protein localization to cell periphery regulation of protein localization to plasma membrane establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport intracellular transport protein export from nucleus establishment of localization protein transport establishment of localization in cell nuclear export intracellular protein transport epidermal cell division cell division regulation of epidermal cell division regulation of cell division developmental process establishment of skin barrier epithelium development skin development skin epidermis development epidermis development anatomical structure development animal organ development tissue development regulation of defense response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms negative regulation of response to biotic stimulus response to stimulus regulation of response to external stimulus response to biotic stimulus response to stress regulation of response to stress innate immune response regulation of immune system process response to external biotic stimulus negative regulation of response to stimulus immune response response to other organism defense response regulation of innate immune response negative regulation of immune response defense response to symbiont response to external stimulus regulation of response to stimulus negative regulation of immune system process immune system process defense response to other organism negative regulation of response to external stimulus negative regulation of defense response regulation of immune response negative regulation of innate immune response cell population proliferation epithelial cell proliferation keratinocyte proliferation cellular response to stimulus signaling intracellular signal transduction cell communication protein kinase A signaling signal transduction cell adhesion cell-cell adhesion regulation of cell adhesion regulation of cell-cell adhesion negative regulation of cell population proliferation regulation of stem cell proliferation negative regulation of stem cell proliferation stem cell proliferation regulation of cell population proliferation positive regulation of cell adhesion regulation of protein modification process regulation of catalytic activity regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of catalytic activity regulation of transferase activity negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process protein metabolic process phosphorus metabolic process protein modification process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process protein phosphorylation phosphorylation cellular metabolic process regulation of protein metabolic process negative regulation of metabolic process negative regulation of molecular function regulation of cellular metabolic process primary metabolic process negative regulation of protein kinase activity regulation of protein kinase activity regulation of macromolecule metabolic process regulation of molecular function regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process metabolic process macromolecule metabolic process negative regulation of phosphorylation regulation of metabolic process positive regulation of nucleocytoplasmic transport positive regulation of establishment of protein localization positive regulation of protein transport positive regulation of intracellular transport regulation of protein export from nucleus regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of nucleocytoplasmic transport regulation of protein transport positive regulation of protein export from nucleus positive regulation of transport positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport cell death apoptotic signaling pathway cellular response to stress DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage apoptotic process intrinsic apoptotic signaling pathway programmed cell death multicellular organismal process keratinization epidermal cell differentiation cellular developmental process epithelial cell differentiation cell differentiation keratinocyte differentiation keratinocyte development cell development epithelial cell development regulation of keratinocyte proliferation negative regulation of keratinocyte proliferation regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cell cycle regulation of cell cycle cellular biosynthetic process RNA metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process apoptotic mitochondrial changes release of cytochrome c from mitochondria organelle organization mitochondrion organization regulation of developmental process regulation of epidermal cell differentiation regulation of cell differentiation regulation of epithelial cell differentiation positive regulation of developmental process multicellular organism development positive regulation of cell differentiation regulation of multicellular organismal process regulation of multicellular organismal development positive regulation of epidermis development positive regulation of epithelial cell differentiation regulation of epidermis development positive regulation of epidermal cell differentiation	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle nucleus intracellular membrane-bounded organelle
B	NPM1 phosphopeptide	protein binding binding histone binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding DNA-binding transcription factor binding NF-kappaB binding RNA binding organic cyclic compound binding rRNA binding nucleic acid binding DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding protein binding binding histone binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding DNA-binding transcription factor binding NF-kappaB binding RNA binding organic cyclic compound binding rRNA binding nucleic acid binding DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding double-stranded DNA binding core promoter sequence-specific DNA binding transcription regulatory region nucleic acid binding ribonucleoprotein complex binding protein-containing complex binding ribosomal large subunit binding ribosome binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity kinase inhibitor activity kinase regulator activity chromatin binding transcription coactivator activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity Tat protein binding unfolded protein binding identical protein binding protein homodimerization activity protein dimerization activity kinase binding enzyme binding protein kinase binding ribosomal small subunit binding	regulation of cellular process regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of transferase activity negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process phosphorus metabolic process protein modification process regulation of cellular process regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of transferase activity negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process phosphorus metabolic process protein modification process negative regulation of protein metabolic process regulation of biological process protein phosphorylation phosphorylation cellular metabolic process regulation of protein metabolic process negative regulation of metabolic process regulation of cellular metabolic process primary metabolic process regulation of molecular function regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification regulation of phosphorus metabolic process metabolic process macromolecule metabolic process regulation of metabolic process regulation of protein modification process regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of cellular process protein metabolic process negative regulation of macromolecule metabolic process negative regulation of protein kinase activity by regulation of protein phosphorylation biological regulation negative regulation of molecular function negative regulation of protein kinase activity regulation of protein kinase activity regulation of macromolecule metabolic process negative regulation of phosphorus metabolic process regulation of phosphorylation cellular process negative regulation of phosphorylation cell cycle G2/M phase transition regulation of cell cycle phase transition regulation of cell cycle process positive regulation of biological process regulation of cell cycle G2/M phase transition cell cycle phase transition positive regulation of cell cycle G2/M phase transition positive regulation of cell cycle positive regulation of cell cycle phase transition positive regulation of cell cycle process cell cycle regulation of cell cycle positive regulation of cellular process cell cycle process protein localization localization cellular localization cellular macromolecule localization macromolecule localization regulation of ribonuclease activity RNA metabolic process nucleic acid metabolic process regulation of endoribonuclease activity nucleobase-containing compound metabolic process regulation of RNA metabolic process regulation of hydrolase activity regulation of nuclease activity regulation of nucleobase-containing compound metabolic process nucleocytoplasmic transport transport nuclear transport establishment of localization in cell intracellular transport establishment of localization microtubule organizing center organization regulation of cellular component organization regulation of centrosome cycle microtubule cytoskeleton organization regulation of microtubule-based process cellular component organization centrosome duplication organelle organization centrosome cycle regulation of centrosome duplication microtubule-based process cellular component organization or biogenesis cytoskeleton organization cellular response to stimulus cellular response to stress response to stimulus response to stress cellular senescence positive regulation of DNA-binding transcription factor activity cellular biosynthetic process positive regulation of NF-kappaB transcription factor activity RNA biosynthetic process positive regulation of molecular function nucleic acid biosynthetic process DNA-templated transcription regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of DNA-binding transcription factor activity regulation of macromolecule biosynthetic process regulation of DNA-templated transcription DNA metabolic process DNA damage response DNA repair regulation of mRNA stability involved in cellular response to UV cellular response to environmental stimulus post-transcriptional regulation of gene expression cellular response to light stimulus regulation of biological quality RNA catabolic process mRNA catabolic process cellular response to radiation response to UV negative regulation of cellular biosynthetic process nucleobase-containing compound catabolic process negative regulation of gene expression negative regulation of macromolecule biosynthetic process nucleic acid catabolic process response to abiotic stimulus cellular response to abiotic stimulus response to light stimulus catabolic process macromolecule catabolic process regulation of mRNA catabolic process regulation of catabolic process mRNA metabolic process regulation of mRNA stability negative regulation of biosynthetic process regulation of RNA stability response to radiation cellular response to UV regulation of mRNA metabolic process positive regulation of DNA-templated transcription positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process transcription by RNA polymerase II positive regulation of biosynthetic process positive regulation of macromolecule metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process cellular response to nitrogen compound regulation of cellular response to stress regulation of translational initiation by eIF2 alpha phosphorylation translational initiation response to organic cyclic compound cellular response to dsRNA regulation of translational initiation cellular response to organic cyclic compound response to nitrogen compound regulation of response to stimulus response to chemical regulation of translation in response to stress regulation of response to stress regulation of eIF2 alpha phosphorylation by dsRNA response to dsRNA translation cellular response to chemical stimulus regulation of translation regulation of translational initiation in response to stress non-membrane-bounded organelle assembly cellular component biogenesis cellular component assembly regulation of centriole replication centriole replication centriole assembly organelle assembly regulation of cellular component biogenesis regulation of organelle organization regulation of organelle assembly establishment of organelle localization ribonucleoprotein complex biogenesis ribosomal subunit export from nucleus protein-containing complex localization ribosome biogenesis ribosomal small subunit export from nucleus organelle localization nuclear export ribosome localization negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death negative regulation of apoptotic process apoptotic process programmed cell death regulation of endodeoxyribonuclease activity regulation of deoxyribonuclease activity regulation of DNA metabolic process ribosomal large subunit export from nucleus signaling signal transduction cell communication protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization chromatin organization protein-containing complex assembly chromatin remodeling nucleosome assembly nucleosome organization cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation positive regulation of protein metabolic process positive regulation of translation positive regulation of gene expression establishment of protein localization nitrogen compound transport protein transport intracellular protein transport ribosomal large subunit biogenesis negative regulation of cell population proliferation regulation of cytoskeleton organization negative regulation of cell cycle negative regulation of centrosome cycle negative regulation of centrosome duplication negative regulation of cellular component organization negative regulation of cytoskeleton organization negative regulation of cell cycle process negative regulation of organelle organization ribosomal small subunit biogenesis ribosome assembly	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol anchoring junction cell junction focal adhesion cell-substrate junction membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol anchoring junction cell junction focal adhesion cell-substrate junction membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle granular component centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton nucleoplasm protein-containing complex spindle pole centrosome spindle spindle pole protein-DNA complex ribonucleoprotein complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR023409	14-3-3 protein, conserved site	Conserved Site
A	IPR023410	14-3-3 domain	Domain
A	IPR037435	14-3-3 protein sigma	Family
A	IPR036815	14-3-3 domain superfamily	Homologous Superfamily
A	IPR000308	14-3-3 protein	Family
B	IPR036824	Nucleoplasmin core domain superfamily	Homologous Superfamily
B	IPR032569	Nucleophosmin, C-terminal	Domain
B	IPR004301	Nucleoplasmin family	Family
B	IPR024057	Nucleoplasmin core domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P31947	esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia atypical teratoid rhabdoid tumor spina bifida diabetes mellitus malignant ependymoma neoplasm of esophagus osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis breast cancer ductal breast carcinoma in situ hepatocellular carcinoma large cell medulloblastoma lung cancer malignant glioma
B	Pharos: P06748	acute myeloid leukemia acute promyelocytic leukemia cancer lung adenocarcinoma lymphomatoid papulosis lung cancer lymphoplasmacytic lymphoma malignant glioma MALT lymphoma myopathy oligodendroglioma plasma cell myeloma congenital anemia psoriasis Disease Models, Animal acute myeloid leukemia acute promyelocytic leukemia cancer lung adenocarcinoma lymphomatoid papulosis lung cancer lymphoplasmacytic lymphoma malignant glioma MALT lymphoma myopathy oligodendroglioma plasma cell myeloma congenital anemia psoriasis Disease Models, Animal glioblastoma large cell medulloblastoma lymphatic system disease malignant colon neoplasm medulloblastoma non-Hodgkin lymphoma osteoarthritis chronic obstructive pulmonary disease primary cutaneous anaplastic large cell lymphoma subependymal giant cell astrocytoma gastric cancer hematologic disease malignant ependymoma malignant pancreatic neoplasm myeloid leukemia non-small cell lung carcinoma atypical teratoid rhabdoid tumor pancreatic ductal adenocarcinoma primitive neuroectodermal tumor diabetes mellitus gastric neoplasm hereditary diffuse gastric adenocarcinoma

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	CSO	Parent Component: CYS RESID: AA0205 PSI-MOD : L-cysteine sulfenic acid MOD:00210
B	SEP	Parent Component: SER RESID: AA0205 , AA0037 PSI-MOD : L-cysteine sulfenic acid MOD:00210 , O-phospho-L-serine MOD:00046

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.