7OBL

Crystal structure of 14-3-3 sigma in complex with PKR phosphopeptide and stabilizer Fusicoccin-A

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	14-3-3	e7oblA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: 14-3-3	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00244	14-3-3 protein (14-3-3)	14-3-3 protein	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	14-3-3 protein sigma	phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity molecular sequestering activity protein sequestering activity kinase binding enzyme binding protein kinase binding cell adhesion molecule binding cadherin binding identical protein binding	regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis positive regulation of cellular process protein localization localization cellular localization cellular macromolecule localization macromolecule localization regulation of localization regulation of protein localization negative regulation of biological process negative regulation of protein localization to plasma membrane negative regulation of cellular process regulation of protein localization to membrane negative regulation of protein localization protein localization to cell periphery protein localization to plasma membrane localization within membrane regulation of protein localization to cell periphery protein localization to membrane negative regulation of protein localization to membrane regulation of cellular localization negative regulation of protein localization to cell periphery regulation of protein localization to plasma membrane establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport intracellular transport protein export from nucleus establishment of localization protein transport establishment of localization in cell nuclear export intracellular protein transport epidermal cell division cell division regulation of epidermal cell division regulation of cell division developmental process establishment of skin barrier epithelium development skin development skin epidermis development epidermis development anatomical structure development animal organ development tissue development regulation of defense response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms negative regulation of response to biotic stimulus response to stimulus regulation of response to external stimulus response to biotic stimulus response to stress regulation of response to stress innate immune response regulation of immune system process response to external biotic stimulus negative regulation of response to stimulus immune response response to other organism defense response regulation of innate immune response negative regulation of immune response defense response to symbiont response to external stimulus regulation of response to stimulus negative regulation of immune system process immune system process defense response to other organism negative regulation of response to external stimulus negative regulation of defense response regulation of immune response negative regulation of innate immune response cell population proliferation epithelial cell proliferation keratinocyte proliferation cellular response to stimulus signaling intracellular signal transduction cell communication protein kinase A signaling signal transduction cell adhesion cell-cell adhesion regulation of cell adhesion regulation of cell-cell adhesion negative regulation of cell population proliferation regulation of stem cell proliferation negative regulation of stem cell proliferation stem cell proliferation regulation of cell population proliferation positive regulation of cell adhesion regulation of protein modification process regulation of catalytic activity regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of catalytic activity regulation of transferase activity negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process protein metabolic process phosphorus metabolic process protein modification process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process protein phosphorylation phosphorylation cellular metabolic process regulation of protein metabolic process negative regulation of metabolic process negative regulation of molecular function regulation of cellular metabolic process primary metabolic process negative regulation of protein kinase activity regulation of protein kinase activity regulation of macromolecule metabolic process regulation of molecular function regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process metabolic process macromolecule metabolic process negative regulation of phosphorylation regulation of metabolic process positive regulation of nucleocytoplasmic transport positive regulation of establishment of protein localization positive regulation of protein transport positive regulation of intracellular transport regulation of protein export from nucleus regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of nucleocytoplasmic transport regulation of protein transport positive regulation of protein export from nucleus positive regulation of transport positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport cell death apoptotic signaling pathway cellular response to stress DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage apoptotic process intrinsic apoptotic signaling pathway programmed cell death multicellular organismal process keratinization epidermal cell differentiation cellular developmental process epithelial cell differentiation cell differentiation keratinocyte differentiation keratinocyte development cell development epithelial cell development regulation of keratinocyte proliferation negative regulation of keratinocyte proliferation regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cell cycle regulation of cell cycle cellular biosynthetic process RNA metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process apoptotic mitochondrial changes release of cytochrome c from mitochondria organelle organization mitochondrion organization regulation of developmental process regulation of epidermal cell differentiation regulation of cell differentiation regulation of epithelial cell differentiation positive regulation of developmental process multicellular organism development positive regulation of cell differentiation regulation of multicellular organismal process regulation of multicellular organismal development positive regulation of epidermis development positive regulation of epithelial cell differentiation regulation of epidermis development positive regulation of epidermal cell differentiation	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle nucleus intracellular membrane-bounded organelle
B	PKR phosphopeptide	kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups RNA binding double-stranded RNA binding binding organic cyclic compound binding nucleic acid binding protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity catalytic activity, acting on a protein protein kinase activity phosphotransferase activity, alcohol group as acceptor kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups RNA binding double-stranded RNA binding binding organic cyclic compound binding nucleic acid binding protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity catalytic activity, acting on a protein protein kinase activity phosphotransferase activity, alcohol group as acceptor adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding non-membrane spanning protein tyrosine kinase activity protein tyrosine kinase activity identical protein binding protein binding enzyme regulator activity phosphatase regulator activity molecular function regulator activity protein phosphatase regulator activity protein serine kinase activity	cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of cellular response to stress positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus stress-activated MAPK cascade cellular response to stress regulation of intracellular signal transduction cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of cellular response to stress positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus stress-activated MAPK cascade cellular response to stress regulation of intracellular signal transduction response to stress regulation of response to stress intracellular signaling cassette signal transduction positive regulation of stress-activated protein kinase signaling cascade positive regulation of cellular process regulation of biological process regulation of signaling positive regulation of stress-activated MAPK cascade positive regulation of MAPK cascade biological regulation regulation of stress-activated protein kinase signaling cascade positive regulation of intracellular signal transduction regulation of MAPK cascade regulation of stress-activated MAPK cascade stress-activated protein kinase signaling cascade regulation of response to stimulus MAPK cascade regulation of cell communication cellular process positive regulation of signaling protein phosphorylation protein autophosphorylation phosphorylation cellular metabolic process primary metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process multicellular organismal process cellular biosynthetic process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of biosynthetic process regulation of cytokine production cytokine production regulation of multicellular organismal process positive regulation of macromolecule metabolic process regulation of biosynthetic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of chemokine production regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression chemokine production positive regulation of chemokine production positive regulation of multicellular organismal process regulation of macromolecule biosynthetic process positive regulation of cytokine production regulation of metabolic process positive regulation of cellular biosynthetic process negative regulation of cell population proliferation cell population proliferation negative regulation of biological process osteoblast proliferation regulation of cell population proliferation negative regulation of cellular process negative regulation of osteoblast proliferation regulation of osteoblast proliferation positive regulation of DNA-binding transcription factor activity positive regulation of NF-kappaB transcription factor activity RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process positive regulation of molecular function nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process regulation of molecular function regulation of DNA-binding transcription factor activity regulation of DNA-templated transcription response to nutrient levels cellular response to amino acid starvation cellular response to starvation cellular response to nutrient levels response to amino acid starvation response to starvation biological process involved in interspecies interaction between organisms response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to external biotic stimulus response to virus cellular response to topologically incorrect protein endoplasmic reticulum unfolded protein response cellular response to unfolded protein response to unfolded protein response to topologically incorrect protein response to endoplasmic reticulum stress translation developmental process regulation of stem cell proliferation cellular developmental process hemopoiesis cell development stem cell proliferation cell differentiation anatomical structure development hematopoietic stem cell proliferation regulation of hematopoietic stem cell proliferation regulation of developmental process regulation of cell differentiation regulation of hematopoietic stem cell differentiation stem cell differentiation regulation of stem cell differentiation hematopoietic stem cell differentiation regulation of hematopoietic progenitor cell differentiation hematopoietic progenitor cell differentiation negative regulation of cellular metabolic process post-transcriptional regulation of gene expression negative regulation of translation negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of protein metabolic process negative regulation of metabolic process negative regulation of cellular biosynthetic process negative regulation of gene expression negative regulation of biosynthetic process regulation of translation negative regulation of macromolecule biosynthetic process negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death negative regulation of apoptotic process apoptotic process programmed cell death positive regulation of response to biotic stimulus regulation of NLRP3 inflammasome complex assembly pattern recognition receptor signaling pathway cellular component biogenesis immune response-activating signaling pathway cellular component assembly inflammasome-mediated signaling pathway positive regulation of immune response canonical inflammasome complex assembly regulation of immune system process positive regulation of immune system process innate immune response-activating signaling pathway immune response regulation of innate immune response regulation of inflammasome-mediated signaling pathway regulation of pattern recognition receptor signaling pathway NLRP3 inflammasome complex assembly positive regulation of defense response activation of innate immune response regulation of immune response inflammatory response activation of immune response cytoplasmic pattern recognition receptor signaling pathway regulation of defense response regulation of response to biotic stimulus regulation of cellular component organization regulation of response to external stimulus intracellular receptor signaling pathway regulation of cytoplasmic pattern recognition receptor signaling pathway cellular component organization regulation of protein-containing complex assembly positive regulation of response to external stimulus immune response-regulating signaling pathway innate immune response protein-containing complex assembly cellular component organization or biogenesis positive regulation of innate immune response protein-containing complex organization regulation of cellular component biogenesis immune system process antiviral innate immune response regulation of non-canonical NF-kappaB signal transduction non-canonical NF-kappaB signal transduction positive regulation of non-canonical NF-kappaB signal transduction negative regulation of viral genome replication viral life cycle negative regulation of viral process regulation of viral process regulation of viral life cycle viral genome replication viral process regulation of viral genome replication response to peptide response to interferon-alpha response to chemical response to cytokine	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol perinuclear region of cytoplasm membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol perinuclear region of cytoplasm membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR023409	14-3-3 protein, conserved site	Conserved Site
A	IPR023410	14-3-3 domain	Domain
A	IPR037435	14-3-3 protein sigma	Family
A	IPR036815	14-3-3 domain superfamily	Homologous Superfamily
A	IPR000308	14-3-3 protein	Family
B	IPR000719	Protein kinase domain	Domain
B	IPR044453	EIF2AK2, second double-stranded RNA binding domain	Domain
B	IPR017441	Protein kinase, ATP binding site	Binding Site
B	IPR050339	Cell Cycle and Stress Response Kinase	Family
B	IPR044452	EIF2AK2, first double-stranded RNA binding domain	Domain
B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
B	IPR014720	Double-stranded RNA-binding domain	Domain
B	IPR008271	Serine/threonine-protein kinase, active site	Active Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P31947	esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia atypical teratoid rhabdoid tumor spina bifida diabetes mellitus malignant ependymoma neoplasm of esophagus osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis breast cancer ductal breast carcinoma in situ hepatocellular carcinoma large cell medulloblastoma lung cancer malignant glioma
B	Pharos: P19525	status epilepticus prostate cancer influenza plasma cell myeloma psoriasis tuberculosis amyotrophic lateral sclerosis cutaneous lupus erythematosus glioblastoma juvenile dermatomyositis malignant ependymoma Non-Convulsive Status Epilepticus pancreatic intraductal papillary-mucinous neoplasm atypical teratoid rhabdoid tumor dermatomyositis status epilepticus prostate cancer influenza plasma cell myeloma psoriasis tuberculosis amyotrophic lateral sclerosis cutaneous lupus erythematosus glioblastoma juvenile dermatomyositis malignant ependymoma Non-Convulsive Status Epilepticus pancreatic intraductal papillary-mucinous neoplasm atypical teratoid rhabdoid tumor dermatomyositis large cell medulloblastoma medulloblastoma osteosarcoma Sjogren syndrome systemic lupus erythematosus Emery-Dreifuss muscular dystrophy lung adenocarcinoma nasopharyngeal carcinoma ovarian cancer

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B	TPO	Parent Component: THR RESID: AA0038 PSI-MOD : O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.