Annotations: 8YN0

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], E [auth A]	PF18117	Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)	Enhanced disease susceptibility 1 protein EP domain	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].	Domain
A [auth B], E [auth A]	PF01764	Lipase (class 3) (Lipase_3)	Lipase (class 3)	-	Family
B [auth E], C	PF18117	Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)	Enhanced disease susceptibility 1 protein EP domain	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].	Domain
B [auth E], C	PF01764	Lipase (class 3) (Lipase_3)	Lipase (class 3)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], E [auth A]	Protein EDS1	identical protein binding protein binding binding protein homodimerization activity protein dimerization activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity hydrolase activity catalytic activity lipase activity	developmental process anatomical structure morphogenesis aerenchyma formation anatomical structure formation involved in morphogenesis anatomical structure development response to decreased oxygen levels response to stimulus response to hypoxia response to stress response to oxygen levels response to abiotic stimulus cellular response to stimulus biological process involved in interspecies interaction between organisms response to other organism developmental process anatomical structure morphogenesis aerenchyma formation anatomical structure formation involved in morphogenesis anatomical structure development response to decreased oxygen levels response to stimulus response to hypoxia response to stress response to oxygen levels response to abiotic stimulus cellular response to stimulus biological process involved in interspecies interaction between organisms response to other organism defense response immune response defense response to symbiont response to external stimulus cell death response to biotic stimulus symbiont-induced defense-related programmed cell death cellular response to stress defense response to other organism immune system process plant-type hypersensitive response innate immune response cellular process response to external biotic stimulus programmed cell death defense response to Gram-negative bacterium defense response to bacterium response to bacterium response to oxidative stress response to singlet oxygen response to chemical response to oxygen-containing compound response to reactive oxygen species primary metabolic process metabolic process lipid metabolic process multicellular organism development multicellular organismal process leaf abscission abscission regulation of biological process regulation of cellular process regulation of hydrogen peroxide metabolic process reactive oxygen species metabolic process biological regulation hydrogen peroxide metabolic process regulation of metabolic process regulation of reactive oxygen species metabolic process signaling response to salicylic acid cell communication signal transduction salicylic acid mediated signaling pathway response to organic cyclic compound cellular response to salicylic acid stimulus systemic acquired resistance cellular response to organic cyclic compound systemic acquired resistance, salicylic acid mediated signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus	protein-containing complex EDS1 disease-resistance complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle plastid organelle intracellular membrane-bounded organelle chloroplast nucleus endoplasmic reticulum protein-containing complex EDS1 disease-resistance complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle plastid organelle intracellular membrane-bounded organelle chloroplast nucleus endoplasmic reticulum endomembrane system
B [auth E], C	Senescence-associated carboxylesterase 101	hydrolase activity, acting on ester bonds carboxylesterase activity carboxylic ester hydrolase activity hydrolase activity catalytic activity	lipid catabolic process catabolic process metabolic process cellular process primary metabolic process lipid metabolic process regulation of defense response positive regulation of response to biotic stimulus regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms response to stimulus regulation of response to external stimulus positive regulation of biological process response to biotic stimulus lipid catabolic process catabolic process metabolic process cellular process primary metabolic process lipid metabolic process regulation of defense response positive regulation of response to biotic stimulus regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms response to stimulus regulation of response to external stimulus positive regulation of biological process response to biotic stimulus positive regulation of response to stimulus defense response to bacterium positive regulation of defense response to bacterium positive regulation of response to external stimulus regulation of response to stress response to stress response to bacterium response to external biotic stimulus regulation of defense response to bacterium regulation of biological process defense response response to other organism biological regulation response to external stimulus regulation of response to stimulus defense response to other organism positive regulation of defense response cellular response to stimulus immune response defense response to symbiont cell death symbiont-induced defense-related programmed cell death cellular response to stress immune system process plant-type hypersensitive response innate immune response programmed cell death regulation of defense response to virus defense response to virus positive regulation of defense response to virus by host regulation of defense response to virus by host response to virus developmental process multicellular organismal process plant organ development plant organ senescence regulation of developmental process positive regulation of leaf senescence regulation of leaf senescence positive regulation of developmental process multicellular organism development anatomical structure development leaf development leaf senescence shoot system development phyllome development system development defense response to Gram-negative bacterium response to oomycetes regulation of defense response to oomycetes positive regulation of defense response to oomycetes defense response to oomycetes leaf abscission abscission regulation of cellular process signaling response to salicylic acid cell communication signal transduction salicylic acid mediated signaling pathway response to organic cyclic compound cellular response to salicylic acid stimulus systemic acquired resistance cellular response to organic cyclic compound response to oxygen-containing compound systemic acquired resistance, salicylic acid mediated signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus response to chemical	protein-containing complex EDS1 disease-resistance complex cellular anatomical structure membrane intracellular anatomical structure cytoplasm membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
D [auth H], F [auth D]	Probable disease resistance protein At5g66890	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to other organism response to stress response to external stimulus response to external biotic stimulus response to biotic stimulus defense response to fungus response to fungus	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], E [auth A]	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
A [auth B], E [auth A]	IPR044214	EDS1-like	Family
A [auth B], E [auth A]	IPR041266	EDS1, EP domain	Domain
A [auth B], E [auth A]	IPR002921	Fungal lipase-type domain	Domain
B [auth E], C	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
B [auth E], C	IPR041266	EDS1, EP domain	Domain
B [auth E], C	IPR002921	Fungal lipase-type domain	Domain
B [auth E], C	IPR044603	Senescence-associated carboxylesterase 101-like	Family
D [auth H], F [auth D]	IPR039203	RGA2/3-like	Unknown
D [auth H], F [auth D]	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
D [auth H], F [auth D]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.