1MLC

MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME COMPLEXED WITH LYSOZYME


X-RAY DIFFRACTION

Crystallization

Crystal Properties
Matthews coefficientSolvent content
2.8957.39

Crystal Data

Unit Cell
Length ( Å )Angle ( ˚ )
a = 99.7α = 90
b = 167.3β = 90
c = 84.7γ = 90
Symmetry
Space GroupP 21 21 21

Diffraction

Diffraction Experiment
ID #Crystal IDScattering TypeData Collection TemperatureDetectorDetector TypeDetailsCollection DateMonochromatorProtocol
11x-rayAREA DETECTORSIEMENS
Radiation Source
ID #SourceTypeWavelength ListSynchrotron SiteBeamline
11.5418

Data Collection

Refinement

Statistics
Diffraction IDStructure Solution MethodResolution (High)Resolution (Low)Cut-off Sigma (F)Number Reflections (Observed)Number Reflections (R-Free)Percent Reflections (Observed)R-Factor (Observed)R-WorkR-FreeMean Isotropic B
X-RAY DIFFRACTION2.572427130.1840.28227.1
Temperature Factor Modeling
Anisotropic B[1][1]Anisotropic B[1][2]Anisotropic B[1][3]Anisotropic B[2][2]Anisotropic B[2][3]Anisotropic B[3][3]
RMS Deviations
KeyRefinement Restraint Deviation
p_chiral_restr167
p_staggered_tor23.1
p_orthonormal_tor20.1
p_planar_tor5.4
p_scangle_it2.04
p_mcangle_it1.56
p_scbond_it1.27
p_mcbond_it0.86
p_multtor_nbd0.27
p_xhyhbond_nbd0.23
RMS Deviations
KeyRefinement Restraint Deviation
p_chiral_restr167
p_staggered_tor23.1
p_orthonormal_tor20.1
p_planar_tor5.4
p_scangle_it2.04
p_mcangle_it1.56
p_scbond_it1.27
p_mcbond_it0.86
p_multtor_nbd0.27
p_xhyhbond_nbd0.23
p_singtor_nbd0.22
p_planar_d0.051
p_angle_d0.041
p_bond_d0.016
p_plane_restr0.01
p_angle_deg
p_hb_or_metal_coord
p_xyhbond_nbd
p_transverse_tor
p_special_tor
Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen AtomsNumber
Protein Atoms8582
Nucleic Acid Atoms
Solvent Atoms210
Heterogen Atoms

Software

Software
Software NamePurpose
XDSdata scaling
PROLSQrefinement
XDSdata reduction