2V0U

n- and c-terminal helices of oat lov2 (404-546) are involved in light-induced signal transduction (cryo dark structure of lov2 (404-546))


X-RAY DIFFRACTION

Starting Model(s)

Initial Refinement Model(s)
TypeSourceAccession CodeDetails
experimental modelPDB 1G28PDB ENTRY 1G28

Crystallization

Crystal Properties
Matthews coefficientSolvent content
2.0840.96

Crystal Data

Unit Cell
Length ( Å )Angle ( ˚ )
a = 35.549α = 90
b = 56.129β = 90
c = 66.778γ = 90
Symmetry
Space GroupP 21 21 21

Diffraction

Diffraction Experiment
ID #Crystal IDScattering TypeData Collection TemperatureDetectorDetector TypeDetailsCollection DateMonochromatorProtocol
11x-ray105CCDMARRESEARCHBENT CONICAL SI-MIRROR (RH COATED)MSINGLE WAVELENGTH
Radiation Source
ID #SourceTypeWavelength ListSynchrotron SiteBeamline
1SYNCHROTRONAPS BEAMLINE 14-BM-CAPS14-BM-C

Data Collection

Overall
ID #Resolution (High)Resolution (Low)Percent Possible (Observed)R Merge I (Observed)Net I Over Average Sigma (I)RedundancyNumber Reflections (All)Number Reflections (Observed)Observed Criterion Sigma (F)Observed Criterion Sigma (I)B (Isotropic) From Wilson Plot
11.450990.05366.726704
Highest Resolution Shell
ID #Resolution (High)Resolution (Low)Percent Possible (All)Percent Possible (Observed)R Merge I (Observed)Mean I Over Sigma (Observed)RedundancyNumber Unique Reflections (All)
11.41.451000.533.76.8

Refinement

Statistics
Diffraction IDStructure Solution MethodCross Validation methodStarting modelResolution (High)Resolution (Low)Number Reflections (Observed)Number Reflections (R-Free)Percent Reflections (Observed)R-Factor (Observed)R-WorkR-FreeR-Free Selection DetailsMean Isotropic B
X-RAY DIFFRACTIONMOLECULAR REPLACEMENTTHROUGHOUTPDB ENTRY 1G281.424.3425307134098.70.1670.1650.197RANDOM13.24
Temperature Factor Modeling
Anisotropic B[1][1]Anisotropic B[1][2]Anisotropic B[1][3]Anisotropic B[2][2]Anisotropic B[2][3]Anisotropic B[3][3]
-0.120.13-0.01
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg29.62
r_dihedral_angle_4_deg12.939
r_dihedral_angle_3_deg11.158
r_dihedral_angle_1_deg6.182
r_scangle_it2.976
r_scbond_it2.109
r_angle_refined_deg1.293
r_mcangle_it1.022
r_mcbond_it0.939
r_angle_other_deg0.887
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg29.62
r_dihedral_angle_4_deg12.939
r_dihedral_angle_3_deg11.158
r_dihedral_angle_1_deg6.182
r_scangle_it2.976
r_scbond_it2.109
r_angle_refined_deg1.293
r_mcangle_it1.022
r_mcbond_it0.939
r_angle_other_deg0.887
r_symmetry_vdw_other0.272
r_nbd_refined0.246
r_symmetry_vdw_refined0.225
r_nbd_other0.188
r_nbtor_refined0.18
r_symmetry_hbond_refined0.152
r_xyhbond_nbd_refined0.115
r_nbtor_other0.084
r_chiral_restr0.078
r_bond_refined_d0.009
r_gen_planes_refined0.005
r_bond_other_d0.002
r_gen_planes_other0.001
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_mcbond_other
r_mcangle_other
r_scbond_other
r_scangle_other
r_long_range_B_refined
r_long_range_B_other
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen AtomsNumber
Protein Atoms1186
Nucleic Acid Atoms
Solvent Atoms173
Heterogen Atoms67

Software

Software
Software NamePurpose
REFMACrefinement
HKL-2000data reduction
SCALEPACKdata scaling
AMoREphasing