N-acetylneuraminate lyase

UniProtKB accession:  P0A6L4

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UniProtKB description:  Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate (PubMed:12711733, PubMed:1646603, PubMed:24521460, PubMed:33895133). Experiments show the true substrate is aceneuramate (linearized Neu5Ac), which forms spontaneously at alkaline pH (PubMed:33895133). Linear aceneuramate can be provided by NanQ (PubMed:33895133). Can also cleave other substrates such as N-glycollylneuraminic acid (GcNeu), but not colominic acid or 2-oxocarboxylic acids such as 2-oxohexanoic acid, 2-oxo-octanoic acid, 2-oxo-3-deoxyoctanoic acid and 2-oxononanoic acid (PubMed:1646603).