Download MendeleyStructural comparison of human and malarial purine nucleoside phosphorylases
Shi, W., Lewandowicz, A., Tyler, P.C., Furneaux, R.H., Almo, S.C., Schramm, V.L.To be published.
1RSZ
Structure of human purine nucleoside phosphorylase in complex with DADMe-Immucillin-H and sulfate
- PDB DOI: https://doi.org/10.2210/pdb1RSZ/pdb
- Classification: TRANSFERASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2003-12-10 Released: 2005-02-22
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.276
- R-Value Work: 0.250
- R-Value Observed: 0.253
Starting Model: experimental
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This is version 2.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Purine nucleoside phosphorylase | 289 | Homo sapiens | Mutation(s): 0 Gene Names: NP, PNP EC: 2.4.2.1 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P00491 (Homo sapiens) Explore P00491 Go to UniProtKB: P00491 | |||||
PHAROS: P00491 GTEx: ENSG00000198805 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P00491 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| DIH Query on DIH | E [auth A] | 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one C12 H17 N4 O3 AFNHHLILYQEHKK-BDAKNGLRSA-O |  | ||
| SO4 Query on SO4 | B [auth A], C [auth A], D [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.276
- R-Value Work: 0.250
- R-Value Observed: 0.253
- Space Group: H 3 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 142.162 | α = 90 |
| b = 142.162 | β = 90 |
| c = 166.387 | γ = 120 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
| CNS | refinement |
Entry History
Deposition Data
- Released Date: 2005-02-22 Deposition Author(s): Shi, W., Lewandowicz, A., Tyler, P.C., Furneaux, R.H., Almo, S.C., Schramm, V.L.
Revision History (Full details and data files)
- Version 1.0: 2005-02-22
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance - Version 2.0: 2018-12-12
Changes: Data collection, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2023-08-23
Changes: Data collection, Database references, Derived calculations, Refinement description
