1W50

Apo Structure of BACE (Beta Secretase)

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.75 Å
  • R-Value Free: 0.283 
  • R-Value Work: 0.244 
  • R-Value Observed: 0.245 

Starting Model: experimental
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This is version 1.3 of the entry. See complete history. 


Literature

Apo and Inhibitor Complex Structures of Bace (Beta-Secretase)

Patel, S., Vuillard, L., Cleasby, A., Murray, C.W., Yon, J.

(2004) J Mol Biol 343: 407

  • DOI: https://doi.org/10.1016/j.jmb.2004.08.018
  • Primary Citation of Related Structures:  
    1W50, 1W51

  • PubMed Abstract: 

    Human BACE, also known as beta-secretase, shows promise as a potential therapeutic target for Alzheimer's disease. We determined the apo structure of BACE to 1.75 A, and a structure of a hydroxyethylamine inhibitor complex derived by soaking. These show significant active-site movements compared to previously described BACE structures. Additionally, the structures reveal two pockets that could be targeted by structure-based drug design.

    • Organizational Affiliation: 

    Astex Technology, 436 Cambridge Science Park, Milton Road, CB4 0QA, UK.


Macromolecules
Find similar proteins by: 
(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
BETA-SECRETASE 1411Homo sapiensMutation(s): 0 
EC: 3.4.23.46
UniProt & NIH Common Fund Data Resources
Find proteins for P56817 (Homo sapiens)
Explore P56817 
Go to UniProtKB:  P56817
PHAROS:  P56817
GTEx:  ENSG00000186318 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP56817
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.75 Å
  • R-Value Free: 0.283 
  • R-Value Work: 0.244 
  • R-Value Observed: 0.245 
  • Space Group: P 61 2 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 103.243α = 90
b = 103.243β = 90
c = 169.108γ = 120
Software Package:
Software NamePurpose
REFMACrefinement
MOSFLMdata reduction
SCALAdata scaling
EPMRphasing

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2004-09-23
    Type: Initial release
  • Version 1.1: 2011-05-08
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2023-12-13
    Changes: Data collection, Database references, Other, Refinement description