Download MendeleyStructure-based engineering of glucose specificity in a family 10 xylanase from Streptomyces olivaceoviridis E-86
Ichinose, H., Diertavitian, S., Fujimoto, Z., Kuno, A., Leggio, L.L., Kaneko, S.(2012) Process Biochem 47: 358-365
2G3I
Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
- PDB DOI: https://doi.org/10.2210/pdb2G3I/pdb
- Classification: HYDROLASE
- Organism(s): Streptomyces olivaceoviridis
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2006-02-20 Released: 2007-03-06
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.192
- R-Value Work: 0.155
- R-Value Observed: 0.156
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Xylanase | 313 | Streptomyces olivaceoviridis | Mutation(s): 2 Gene Names: Xylanase EC: 3.2.1.8 |  | |
UniProt | |||||
Find proteins for Q7SI98 (Streptomyces olivaceoviridis) Explore Q7SI98 Go to UniProtKB: Q7SI98 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q7SI98 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 Query on PO4 | B [auth A] C [auth A] D [auth A] E [auth A] F [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.192
- R-Value Work: 0.155
- R-Value Observed: 0.156
- Space Group: P 63
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 119.317 | α = 90 |
| b = 119.317 | β = 90 |
| c = 54.355 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| MOSFLM | data reduction |
| CCP4 | data scaling |
Entry History
Deposition Data
- Released Date: 2007-03-06 Deposition Author(s): Diertavitian, S., Kaneko, S., Fujimoto, Z., Kuno, A., Johansson, E., Lo Leggio, L.
Revision History (Full details and data files)
- Version 1.0: 2007-03-06
Type: Initial release - Version 1.1: 2008-05-01
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2012-02-29
Changes: Database references - Version 1.4: 2024-04-03
Changes: Data collection, Database references, Derived calculations, Refinement description
