Download MendeleyHow GPCRs activate G proteins: Structural changes form C-terminal tail to GDP binding pocket
Morikawa, T., Muroya, A., Sugio, S., Wakamatsu, K., Kohno, T.To be published.
2ZJY
Structure of the K349P mutant of Gi alpha 1 subunit bound to ALF4 and GDP
- PDB DOI: https://doi.org/10.2210/pdb2ZJY/pdb
- Classification: HYDROLASE
- Organism(s): Rattus norvegicus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2008-03-11 Released: 2009-03-24
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.260
- R-Value Work: 0.181
- R-Value Observed: 0.181
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Guanine nucleotide-binding protein G(i), alpha-1 subunit | 356 | Rattus norvegicus | Mutation(s): 1 Gene Names: Gnai1, Gnai-1 EC: 3.6.5.1 (PDB Primary Data), 3.6.5 (UniProt) |  | |
UniProt | |||||
Find proteins for P10824 (Rattus norvegicus) Explore P10824 Go to UniProtKB: P10824 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P10824 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GDP Query on GDP | D [auth A] | GUANOSINE-5'-DIPHOSPHATE C10 H15 N5 O11 P2 QGWNDRXFNXRZMB-UUOKFMHZSA-N |  | ||
| ALF Query on ALF | B [auth A] | TETRAFLUOROALUMINATE ION Al F4 UYOMQIYKOOHAMK-UHFFFAOYSA-J |  | ||
| MG Query on MG | C [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.260
- R-Value Work: 0.181
- R-Value Observed: 0.181
- Space Group: P 32 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 79.375 | α = 90 |
| b = 79.375 | β = 90 |
| c = 105.297 | γ = 120 |
| Software Name | Purpose |
|---|---|
| CNS | refinement |
| CrystalClear | data collection |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2009-03-24 Deposition Author(s): Morikawa, T., Muroya, A., Sugio, S., Wakamatsu, K., Kohno, T.
Revision History (Full details and data files)
- Version 1.0: 2009-03-24
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2021-11-10
Changes: Database references, Derived calculations - Version 1.3: 2024-05-29
Changes: Data collection
