2ZZV

Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate

(2009) J Mol Biol 392: 559-565

• PubMed: 19631222 Search on PubMed
• DOI: https://doi.org/10.1016/j.jmb.2009.07.043
• Primary Citation of Related Structures:  
  2ZZV, 2ZZW, 2ZZX


• PubMed Abstract: 
  

  Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.

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Organizational Affiliation: 

Department of Chemistry, Kyoto University, Sakyo-ku, Japan.