3T4O

Arabidopsis histidine kinase 4 sensor domain in complex with dihydrozeatin

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.75 Å
  • R-Value Free: 0.202 
  • R-Value Work: 0.179 
  • R-Value Observed: 0.181 

Starting Model: experimental
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This is version 1.3 of the entry. See complete history. 


Literature

Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4.

Hothorn, M., Dabi, T., Chory, J.

(2011) Nat Chem Biol 7: 766-768

  • DOI: https://doi.org/10.1038/nchembio.667
  • Primary Citation of Related Structures:  
    3T4J, 3T4K, 3T4L, 3T4O, 3T4Q, 3T4S, 3T4T

  • PubMed Abstract: 

    Cytokinins are classic hormones that orchestrate plant growth and development and the integrity of stem cell populations. Cytokinin receptors are eukaryotic sensor histidine kinases that are activated by both naturally occurring adenine-type cytokinins and urea-based synthetic compounds. Crystal structures of the Arabidopsis thaliana histidine kinase 4 sensor domain in complex with different cytokinin ligands now rationalize the hormone-binding specificity of the receptor and may spur the design of new cytokinin ligands.

    • Organizational Affiliation: 

    Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA.


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Histidine kinase 4
A, B
270Arabidopsis thalianaMutation(s): 0 
Gene Names: AHK4, At2g01830, CRE1, RAW1, T23K3.2, WOL
EC: 2.7.13.3 (PDB Primary Data), 3.1.3.16 (PDB Primary Data)
UniProt
Find proteins for Q9C5U0 (Arabidopsis thaliana)
Explore Q9C5U0 
Go to UniProtKB:  Q9C5U0
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9C5U0
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.75 Å
  • R-Value Free: 0.202 
  • R-Value Work: 0.179 
  • R-Value Observed: 0.181 
  • Space Group: P 32 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 60.01α = 90
b = 60.01β = 90
c = 297.86γ = 120
Software Package:
Software NamePurpose
ADSCdata collection
PHASERphasing
REFMACrefinement
XDSdata reduction
XSCALEdata scaling

Structure Validation

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Ligand Structure Quality Assessment 


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Entry History 

Deposition Data

  • Released Date: 2011-10-05 
    • Deposition Author(s): Hothorn, M.

Revision History  (Full details and data files)

  • Version 1.0: 2011-10-05
    Type: Initial release
  • Version 1.1: 2011-12-14
    Changes: Database references
  • Version 1.2: 2023-09-13
    Changes: Data collection, Database references, Derived calculations, Refinement description
  • Version 1.3: 2024-11-20
    Changes: Structure summary