Download MendeleyKinetic and Structural Characterization of Inducible Nitric Oxide Synthase Substituted With Mesoheme
Hannibal, L., Page, R.C., Bolisetty, K., Yu, Z., Misra, S., Stuehr, D.J.To be published.
4JS9
Structural Characterization of Inducible Nitric Oxide Synthase Substituted With Mesoheme
- PDB DOI: https://doi.org/10.2210/pdb4JS9/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Mus musculus
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2013-03-22 Released: 2014-04-23
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.78 Å
- R-Value Free: 0.290
- R-Value Work: 0.250
- R-Value Observed: 0.252
Starting Model: experimental
View more details
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Nitric oxide synthase, inducible | 431 | Mus musculus | Mutation(s): 0 Gene Names: Inosl, Nos2 EC: 1.14.13.39 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P29477 (Mus musculus) Explore P29477 Go to UniProtKB: P29477 | |||||
IMPC: MGI:97361 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P29477 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MH0 Query on MH0 | C [auth A], F [auth B] | Mesoheme C34 H36 Fe N4 O4 CKMCSMAXNUVLQI-RGGAHWMASA-N |  | ||
| H4B Query on H4B | D [auth A], G [auth B] | 5,6,7,8-TETRAHYDROBIOPTERIN C9 H15 N5 O3 FNKQXYHWGSIFBK-RPDRRWSUSA-N |  | ||
| SO4 Query on SO4 | E [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.78 Å
- R-Value Free: 0.290
- R-Value Work: 0.250
- R-Value Observed: 0.252
- Space Group: P 61 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 215.039 | α = 90 |
| b = 215.039 | β = 90 |
| c = 115.547 | γ = 120 |
| Software Name | Purpose |
|---|---|
| StructureStudio | data collection |
| PHENIX | model building |
| PHENIX | refinement |
| d*TREK | data reduction |
| d*TREK | data scaling |
| PHENIX | phasing |
Entry History
Deposition Data
- Released Date: 2014-04-23 Deposition Author(s): Hannibal, L., Page, R.C., Bolisetty, K., Yu, Z., Misra, S., Stuehr, D.J.
Revision History (Full details and data files)
- Version 1.0: 2014-04-23
Type: Initial release - Version 1.1: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description
