Download MendeleyStructural insights into protein splicing inhibition by platinum therapeutics as potential anti-microbials
Chan, H., Pearson, S., Green, C.M., Li, Z., Zhang, J., Lippard, S., Belfort, G., Shekhtman, A., Li, H.M., Belfort, M.To be published.
5K08
RecA mini intein-Zeise's salt complex
- PDB DOI: https://doi.org/10.2210/pdb5K08/pdb
- Classification: HYDROLASE
- Organism(s): Mycobacterium tuberculosis
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2016-05-17 Released: 2016-09-21
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), National Institutes of Health/National Cancer Institute (NIH/NCI)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.210
- R-Value Work: 0.180
- R-Value Observed: 0.182
Starting Model: experimental
View more details
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| RecA mini intein | 142 | Mycobacterium tuberculosis | Mutation(s): 0 |  | |
UniProt | |||||
Find proteins for P9WHJ3 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WHJ3 Go to UniProtKB: P9WHJ3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P9WHJ3 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZPT Query on ZPT | B [auth A], C [auth A] | Trichloro(ethene)platinate(II) C2 H4 Cl3 Pt LJEKXYSRMGLQBW-UHFFFAOYSA-K |  | ||
| TCE Query on TCE | E [auth A] | 3,3',3''-phosphanetriyltripropanoic acid C9 H15 O6 P PZBFGYYEXUXCOF-UHFFFAOYSA-N |  | ||
| SO4 Query on SO4 | D [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.210
- R-Value Work: 0.180
- R-Value Observed: 0.182
- Space Group: P 21 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 57.687 | α = 90 |
| b = 63.93 | β = 90 |
| c = 37.307 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| SCALEPACK | data scaling |
| PDB_EXTRACT | data extraction |
| DENZO | data reduction |
| PHENIX | phasing |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM39422 |
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM44844 |
| National Institutes of Health/National Cancer Institute (NIH/NCI) | United States | CA039442 |
Revision History (Full details and data files)
- Version 1.0: 2016-09-21
Type: Initial release - Version 1.1: 2017-09-20
Changes: Author supporting evidence, Derived calculations - Version 1.2: 2019-12-04
Changes: Author supporting evidence - Version 1.3: 2023-09-27
Changes: Data collection, Database references, Refinement description
