6JFY

Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.

(2019) Sci Rep 9: 10254-10254

• PubMed: 31311973 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1038/s41598-019-46770-z
• Primary Citation of Related Structures:  
  6JFY, 6JFZ, 6JMV


• PubMed Abstract: 
  

  GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission. For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy. While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation. Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties. The mutational analysis also identified putative N-glycan interacting residues. Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.

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Organizational Affiliation: 

Laboratory of Membrane Protein Biology, National Centre for Cell Science, NCCS Complex, S. P. Pune University, Maharashtra, Pune, 411007, India.