Download MendeleyDecomposition of PET film by MHETase using Exo-PETase function
Sagong, H.-Y., Seo, H., Kim, T., Son, H., Joo, S., Lee, S., Kim, S., Woo, J.-S., Hwang, S., Kim, K.-J.(2020) ACS Catal 10: 4805-4812
6JTT
MHETase in complex with BHET
- PDB DOI: https://doi.org/10.2210/pdb6JTT/pdb
- Classification: HYDROLASE
- Organism(s): Piscinibacter sakaiensis
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2019-04-12 Released: 2020-04-15
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.51 Å
- R-Value Free: 0.226
- R-Value Work: 0.190
- R-Value Observed: 0.192
This is version 1.2 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Mono(2-hydroxyethyl) terephthalate hydrolase | 621 | Piscinibacter sakaiensis | Mutation(s): 0 EC: 3.1.1.102 |  | |
UniProt | |||||
Find proteins for A0A0K8P8E7 (Piscinibacter sakaiensis) Explore A0A0K8P8E7 Go to UniProtKB: A0A0K8P8E7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A0K8P8E7 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| C8X Query on C8X | F [auth B] | bis(2-hydroxyethyl) benzene-1,4-dicarboxylate C12 H14 O6 QPKOBORKPHRBPS-UHFFFAOYSA-N |  | ||
| C9C Query on C9C | D [auth A], H [auth C] | 4-(2-hydroxyethyloxycarbonyl)benzoic acid C10 H10 O5 BCBHDSLDGBIFIX-UHFFFAOYSA-N |  | ||
| CA Query on CA | E [auth A], G [auth B], I [auth C] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.51 Å
- R-Value Free: 0.226
- R-Value Work: 0.190
- R-Value Observed: 0.192
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 215.259 | α = 90 |
| b = 173.799 | β = 119.51 |
| c = 106.671 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data reduction |
| PHASER | phasing |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2020-04-15 Deposition Author(s): Sagong, H.-Y., Seo, H., Kim, K.-J.
Revision History (Full details and data files)
- Version 1.0: 2020-04-15
Type: Initial release - Version 1.1: 2020-10-28
Changes: Database references - Version 1.2: 2024-11-06
Changes: Data collection, Database references, Structure summary
