Download MendeleyLiquid application method for time-resolved analyses by serial synchrotron crystallography.
Mehrabi, P., Schulz, E.C., Agthe, M., Horrell, S., Bourenkov, G., von Stetten, D., Leimkohl, J.P., Schikora, H., Schneider, T.R., Pearson, A.R., Tellkamp, F., Miller, R.J.D.(2019) Nat Methods 16: 979-982
- PubMed: 31527838
- DOI: https://doi.org/10.1038/s41592-019-0553-1
- Primary Citation of Related Structures:
6QNB, 6QNC, 6QND, 6QNH, 6QNI, 6QNJ, 6RNB, 6RNC, 6RND, 6RNF - PubMed Abstract:
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
Organizational Affiliation:
Max-Planck-Institute for Structure and Dynamics of Matter, Department for Atomically Resolved Dynamics, Hamburg, Germany.
