Download MendeleyNeutron crystallographic analysis of the nucleotide-binding domain of Hsp72 in complex with ADP.
Yokoyama, T., Fujii, S., Ostermann, A., Schrader, T.E., Nabeshima, Y., Mizuguchi, M.(2022) IUCrJ 9: 562-572
7F50
X-ray crystal structure of Y149A mutated Hsp72-NBD in complex with AMPPnP
- PDB DOI: https://doi.org/10.2210/pdb7F50/pdb
- Classification: HYDROLASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2021-06-21 Released: 2022-06-29
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.70 Å
- R-Value Free: 0.227
- R-Value Work: 0.185
- R-Value Observed: 0.188
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Heat shock 70 kDa protein 1B | 386 | Homo sapiens | Mutation(s): 1 Gene Names: HSPA1B, HSP72 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P0DMV9 (Homo sapiens) Explore P0DMV9 Go to UniProtKB: P0DMV9 | |||||
PHAROS: P0DMV9 GTEx: ENSG00000204388 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0DMV9 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ANP (Subject of Investigation/LOI) Query on ANP | E [auth A] | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER C10 H17 N6 O12 P3 PVKSNHVPLWYQGJ-KQYNXXCUSA-N |  | ||
| CL (Subject of Investigation/LOI) Query on CL | D [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| MG (Subject of Investigation/LOI) Query on MG | B [auth A], C [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.70 Å
- R-Value Free: 0.227
- R-Value Work: 0.185
- R-Value Observed: 0.188
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 45.827 | α = 90 |
| b = 61.968 | β = 90 |
| c = 142.779 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XSCALE | data scaling |
| PHENIX | phasing |
Entry History
Deposition Data
- Released Date: 2022-06-29 Deposition Author(s): Yokoyama, T., Fujii, S., Nabeshima, Y., Mizuguchi, M.
Revision History (Full details and data files)
- Version 1.0: 2022-06-29
Type: Initial release - Version 1.1: 2022-09-14
Changes: Database references - Version 1.2: 2023-11-29
Changes: Data collection, Refinement description
