Download MendeleyX-ray crystallographic structure of a complex between a synthetic protease of human immunodeficiency virus 1 and a substrate-based hydroxyethylamine inhibitor.
Swain, A.L., Miller, M.M., Green, J., Rich, D.H., Schneider, J., Kent, S.B., Wlodawer, A.(1990) Proc Natl Acad Sci U S A 87: 8805-8809
- PubMed: 2247451
- DOI: https://doi.org/10.1073/pnas.87.22.8805
- Primary Citation of Related Structures:
7HVP - PubMed Abstract:
The structure of a crystal complex of the chemically synthesized protease of human immunodeficiency virus 1 with a heptapeptide-derived inhibitor bound in the active site has been determined. The sequence of the inhibitor JG-365 is Ac-Ser-Leu-Asn-Phe-psi[CH(OH)CH2N]-Pro-Ile-Val-OMe; the Ki is 0.24 nM. The hydroxyethylamine moiety, in place of the normal scissile bond of the substrate, is believed to mimic a tetrahedral reaction intermediate. The structure of the complex has been refined to an R factor of 0.146 at 2.4-A resolution by using restrained least squares with rms deviations in bond lengths of 0.02 A and bond angles of 4. The bound inhibitor diastereomer has the S configuration at the hydroxyethylamine chiral carbon, and the hydroxyl group is positioned between the active site aspartate carboxyl groups within hydrogen bonding distance. Comparison of this structure with a reduced peptide bond inhibitor-protease complex indicates that these contacts confer the exceptional binding strength of JG-365.
Organizational Affiliation:
Crystallography Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
