Download MendeleyCrystal structure of divalent Mg+2 dependent Mycobacterium abscessus tRNA (m1 G37) Methyltransferase (TrmD)
Prucha, G.R., Ismail, M., Suske, A., Perez, A., Bolen, R., Jayaraman, S., Stojanoff, V., Halloran, J.To be published.
7REZ
MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE
- PDB DOI: https://doi.org/10.2210/pdb7REZ/pdb
- Classification: TRANSFERASE
- Organism(s): Mycobacteroides abscessus
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2021-07-13 Released: 2023-01-18
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Department of Energy (DOE, United States), Other private
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 Å
- R-Value Free: 0.216
- R-Value Work: 0.186
- R-Value Observed: 0.188
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| tRNA (guanine-N(1)-)-methyltransferase | 233 | Mycobacteroides abscessus | Mutation(s): 0 Gene Names: trmD, SAMEA2161603_03547, SAMEA2275821_02238, SAMEA2275847_06627 EC: 2.1.1.228 |  | |
UniProt | |||||
Find proteins for A0A1M9INC4 (Mycobacteroides abscessus subsp. abscessus) Explore A0A1M9INC4 Go to UniProtKB: A0A1M9INC4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A1M9INC4 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SAH (Subject of Investigation/LOI) Query on SAH | C [auth A], D [auth B] | S-ADENOSYL-L-HOMOCYSTEINE C14 H20 N6 O5 S ZJUKTBDSGOFHSH-WFMPWKQPSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 Å
- R-Value Free: 0.216
- R-Value Work: 0.186
- R-Value Observed: 0.188
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 74.242 | α = 90 |
| b = 79.094 | β = 90 |
| c = 86.631 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| ADDREF | data reduction |
| XDS | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-01-18 Deposition Author(s): Prucha, G.R., Ismail, M., Suske, A., Das, B., Oz, M., Perez, A., Bolen, R., Jayaraman, S., Stojanoff, V., Halloran, J.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | P30GM133893 |
| Department of Energy (DOE, United States) | United States | DE-SC0012704 |
| Department of Energy (DOE, United States) | United States | KP1605010 |
| Other private | United States | -- |
Revision History (Full details and data files)
- Version 1.0: 2023-01-18
Type: Initial release - Version 1.1: 2024-05-22
Changes: Data collection
