Download MendeleyFunctional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated the Engineering Enhancement of Sugar Donor Promiscuity
Huang, W., He, Y., Jiang, R., Deng, Z., Long, F.(2022) ACS Catal : 2927-2937
7W1B
Glycosyltransferase
- PDB DOI: https://doi.org/10.2210/pdb7W1B/pdb
- Classification: TRANSFERASE
- Organism(s): Calotropis gigantea
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2021-11-19 Released: 2022-03-02
- Funding Organization(s): Not funded
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 Å
- R-Value Free: 0.221
- R-Value Work: 0.179
- R-Value Observed: 0.181
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Glycosyltransferase | 480 | Calotropis gigantea | Mutation(s): 0 EC: 2.4.1 |  | |
UniProt | |||||
Find proteins for A0A385Z7H9 (Calotropis gigantea) Explore A0A385Z7H9 Go to UniProtKB: A0A385Z7H9 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A385Z7H9 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| UDP Query on UDP | B [auth A] | URIDINE-5'-DIPHOSPHATE C9 H14 N2 O12 P2 XCCTYIAWTASOJW-XVFCMESISA-N |  | ||
| DTX (Subject of Investigation/LOI) Query on DTX | D [auth A] | DIGITOXIGENIN C23 H34 O4 XZTUSOXSLKTKJQ-CESUGQOBSA-N |  | ||
| TRS Query on TRS | C [auth A] | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C4 H12 N O3 LENZDBCJOHFCAS-UHFFFAOYSA-O |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 Å
- R-Value Free: 0.221
- R-Value Work: 0.179
- R-Value Observed: 0.181
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 53.351 | α = 90 |
| b = 74.141 | β = 95.76 |
| c = 63.588 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
| Aimless | data scaling |
| PHENIX | phasing |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| Not funded | -- |
Revision History (Full details and data files)
- Version 1.0: 2022-03-02
Type: Initial release - Version 1.1: 2023-11-29
Changes: Data collection, Refinement description
